PREVALENCE OF SENSITIZATION TO AIRBORNE ALLERGENS IN PATIENTS WITH ALLERGIC RHINITIS AND ASTHMA IN UKRAINE

Background. The distribution of sensitization among children and adolescence with allergic rhinitis (AR) and asthma and their impact on various allergic symptoms have been analyzed. Aim: investigate the distribution of sensitization among children with AR and asthma of the Ukrainian population. Material and methods. The study involved 280 children with AR and asthma aged 2–18 years. They were tested for sIgE on the most common allergens among Ukrainians - Dermatophagoides pteronyssinus, Dermatophagoides farinae, dog, cat, cockroach, birch (t03, Bet v1, Bet v2, Bet v4), plane tree, timothy (g06, Phl p1, Phl , Phl p6, Phl p7, Phl p12), ragweed, mugwort  and Alternaria. Measurements of sIgE were performed by Western blotting according to the manufacturer's protocol (Simesta-Medivis, Ukraine-Germany). Results. Among the sensitized subjects, 165 were sensitive to pollen allergens, sIgE was positive to birch in 64 subjects (22.5%), to mugwort ‒ 65 subjects (25.4%), ragweed ‒ 110 subjects (42.5%), timothy ‒ 69 subjects (24.6%), plane tree ‒ 12 subjects (5%). Positive sIgE to feline allergen was found in 114 (43.3%), dogs ‒ 77 (32.1%), Dermatophagoides pteronyssinus ‒ 66 (24.6%), Dermatophagoides farinae ‒ 32 (12%), Alternaria ‒ 38 (15 %), cockroaches ‒ 25 (10%). Conclusions. Sensitization to airborne allergens is significantly associated with asthma and AR. The most important inhalant allergens consist of pollens (trees, grasses, and weeds), house dust mites, molds and animal dander. Among sensitive subjects to pollen allergens, allergy to ragweed was most spread

SIGNIFICANCE OF TOLL-LIKE RECEPTOR-2 POLYMORPHISM rs4696480 FOR THE DEVELOPMENT OF FOOD ALLERGY IN CHILDREN WITH ATOPIC DERMATITIS

Relevance. Food allergy is one of the most common chronic diseases in children. Toll-like receptors may be unique in the development of food allergies due to their expression by intestinal epithelial and dendritic cells. Objective. The aim of this study was to investigate the relationship between the rs4696480 polymorphism in TLR2 and the presence of food allergy in children with atopic dermatitis. Material and methods. The study included 103 patients with atopic dermatitis and 84 healthy children. Polymorphism genotyping was performed in the group of patients and the control group using real-time PCR. Food sensitization was determined by presence sIgE to food allergens by the immunochemiluminescent method using an ImmunoCAP 100 (Thermo Fisher Scientific Inc., Phadia, Sweden). The presence of food allergy was determined by a detailed clinical history and review of clinical records. Results. In the subgroup of children with food allergies, 9 children had the AA genotype, 7 patients had the heterozygous variant, and 3 children had the TT genotype. Among patients without food allergy, 20 patients had the AA genotype, 39 were heterozygotes, and 25 had the TT genotype. The OR assessment demonstrated that the AA genotype of the rs4696480 polymorphism is reliably associated with the development of food allergy in children with atopic dermatitis, OR=2,880 (1,0271-8,0757). Conclusion. The rs4696480 polymorphism in TLR2 gene is associated with the development of food allergy in Ukrainian children with atopic dermatitis

Status of child population health — the future of the country (part 1)

The article of the team of scientists of pediatricians and organizers of pediatric health care of Ukraine is devoted to the study of the main trends in the health status of children over last 22 years. The results of the analysis of the incidence and prevalence of diseases among Ukrainian child population, infant mortality rates over the past two decades demonstrates that they remain significantly higher than the average European indicators against the background of a progressive decrease in the number of child population at 3,16 million people. The prevalence of childhood diseases in Ukraine over past 22 years has grown by 41 %, the incidence of childhood diseases arose by 36 %. The article considers the ways of solving a number of new social and medico-ecological problems that negatively affect child health

Child health status — the future of the country (part 2)

The article deals with the problems of increasing the incidence and prevalence of childhood diseases during 1994–2016 against the background of a global reduction in the staffing of children’s specialists by 1.7 times, with an extremely inadequate supply of the regions of the South-East, Donetsk and Luhansk regions. All of this has a negative impact on the proper medical care of the child population, including the detection of childhood diseases. The authors substantiate the need to restore the personnel and material-technical potential of the pediatric service, the development of modern diagnostic and treatment technologies, the preservation of immunization and the prevention of disability of common childhood diseases that is an important component of the national health care and safety system of the country as a whole