The effect of a high-calorie diet on the total content of chemical elements and metal-ligand forms of zinc in the blood serum and liver of Wistar rats

Background. Worldwide, there is a rapid increase in the number of people suffering from various forms of carbohydrate and lipid metabolism disorders. Modern studies show that the transport, distribution, excretion and accumulation of chemical elements in these types of metabolic disorders change in different ways and affect the further functional state of the body differently.The aim. To evaluate the level of macro- and microelements in the blood serum and liver, as well as the content of metal-ligand forms of zinc in the blood serum of a Wistar rat in a high-calorie diet.Materials and methods. Thirty male rats were selected for the experiment, from which two groups were formed: control (n = 15) and experimental (n = 15). The animals of the control group received the basic diet (270 kcal/100 g), and the animals of  the experimental group received a high-calorie diet. During the  experiment, the caloric content of the diet of the experimental group gradually increased from the caloric content of the total diet. During the study, body weight, biochemical parameters of blood and urine were evaluated. The analysis of macro- and microelements in the samples was carried out using inductively coupled plasma mass spectrometry. Determination of the content of individual zinc compounds in blood serum was carried out using a combination of a chromatograph and a mass spectrometer.Results. It was found that a high-calorie diet led to a decrease in the level of iron, chromium, iodine, zinc, potassium, calcium, and an increase in vanadium in blood serum. In the liver, there was a decrease in the level of lithium and an increase in  the  level of calcium, vanadium, chromium, iron, zinc, cobalt. When assessing the chemical forms of zinc in the blood serum, a percentage increase in the albumin fraction was recorded against the background of a decrease in amino acid complexes and low-molecular-weight forms of zinc.Conclusion. The data obtained suggest that a high-calorie diet leads to an imbalance of chemical elements, which can serve as one of the triggers for dysregulation of a number of physiological functions of the body