KIR-HLAC genotyping in married couples with early reproductive losses of unknown genesis

Aim. KIR-HLAC genotyping in married couples with early idiopathic pregnancy loss. Methods. DNA extraction and purification, PCR-SSP, agarose gel electrophoresis. Results. The spectrum of KIR genes was analyzed and the frequency of KIR genotypes in women with early reproductive losses was established. The most common (77.78 %) was the AB genotype, 20.37 % had the AA genotype, and 1.85 % had the BB genotype. HLAC genotyping of couples with regular early reproductive losses showed the C1/C2 genotype of the HLAC gene in 40.74 % of women and 44.44 % of men. The frequency of C1/C1 genotype in women was 27.78% versus 38.89 % in men. The C2/C2 genotype of the HLAC gene was detected in 31.48 % of women and 12.96 % of men. According to the results of KIR-HLAC analysis of genotyping of married couples with early reproductive losses, a high/significant risk of reproductive losses of immunological genesis was found in 55.56 % of cases. Conclusions. KIR-HLAC genotyping is a genetic test that allows to assess the risks of the embryo being rejected by the maternal immune system, and thus to direct medical interventions in order to achieve a successful pregnancy.&#x0D; Keywords: early reproductive losses, KIR, HLAC.</jats:p

Allocation features and frequency of KIR-genes in women with regular early reproductive losses

Aim. KIR (killer cell immunoglobulin-like receptors) are the transmembranous glycoprotein receptors, role of which is to activate or inhibit the functional activity of cells, the surfaces of which they are located on. Genetically determined balance between KIR-activating and KIR-inhibiting receptors can contribute to some diseases predisposition. In this work we have studied the spectrum and frequency of KIR-genes among women with regular early idiopathic reproductive failures. Methods. Gene typing was conducted by PCR-SSP method. Results. Typing of KIR-genes was conducted in 31 DNA samples, extracted from peripheral blood cells from women without reproductive losses and in 38 DNA samples, extracted from peripheral blood cells from women with early reproductive losses of idiopathic nature. We found, that in women with reproductive failures genotypes of KIR-genes were characterized by decreased amount of activating genes. The presence of 2DL3 and 2DL5 genes increases the risk of early reproductive failures in 2 and 3 times, respectively. Conclusions. The shift of repertoire of KIR-genes into the domination of inhibitory genes and the spectrum features of KIR-genes can increase the risk of early reproductive failures.&#x0D; Keywords: KIR-genes, recurrent pregnancy loss, PCR-SSP.</jats:p