THE DEPOSIT OF ANTIBODY’S PROLIFERATIVE ACTIVITY IN THE IMMUNE RESPONSE AND ITS RESISTANCE TO INHIBITORS OF PROLIFERATION

The effect of hyperstimulation of the primary immune response was showed by additional introducing of antigen in the late log-phase of the primary IgM-response. The multiple increase of IgM-and IgG-antibody productive cells (АРС) in the spleen during the primary response was accompanied by suppression of anamnestic response. The injection of hydroxyurea (an inhibitor of DNA synthesis) together with the additional introduction of the antigen at the late log-phase of the primary IgM-response reduces the rise of IgM-APC, but does not abolish the stimulation of the primary and secondary IgG-APC formation. This fact indicates that proliferative processes play an important role in the stimulation of IgM-response induced by reinjection of the antigen. The stimulation of proliferation of B-lymphocytes which differentiate into antibody productive cells significantly reduces the pool of memory cells and consequently, suppresses secondary immune response

LYMPHOPENIA AS A FACTOR INVOLVED IN THE AUTOIMMUNITY DEVELOPMENT IN MURINE GRAFT VS. HOST DISEASE

Syngeneic splenocyte transplantation leads to lymphopenia development in intact mice owing to decreased proliferative activity of bone marrow hematopoietic progenitor cells. In the mice with induced chronic graft-versus-host disease (cGVHD) transfer of syngeneic lymphocytes has little or no effect on the level of already existing lymphopenia, but increases its duration. These results are in close agreement with the received evidences that transfer of syngeneic cells increases frequency of autoimmune glomerulonephritis at cGVHD. Thus, results of research speak in favor of conclusion that lymphopenia and initiated by it homeostatic proliferation play a pathogenetic role in the development of autoimmune reactions at cGVHD