Prospects for the Use of Thermal Extracts of Brucella abortusI-206 in S-and L-Forms in the Diagnosis and Prevention of Brucellosis

Currently, one of the topical areas of research is the development of new antigen preparations for the specific diagnosis and prevention of brucellosis, since indication of the pathogen and prevention of the disease is complicated by the ability of brucella to dissociate, and live vaccines used for specific prevention of brucellosis have residual virulence. Thermal extracts (TE) obtained from Brucella abortus I-206 in the L- and S-form can be used as such promising antigens. It is known that TE in the L- and S-forms have immunogenic properties, as well as a modulating effect on the proliferation of immunocompetent cells, morphological changes in the immunocompetent organs of experimental animals.The aimof the work is to study the effect of Brucella abortus thermal extracts in L- and S-forms on the functional state of the cells of experimental animals.Materials  and  methods. The  study  was  performed  on  100  certified  white  mice.  As  objects  of  study,  we  used  the B. abortus I-206 TE in L- and S-forms. Evaluation of the effect of antigenic drugs on the functional state of phagocytes of laboratory animals in vitro was performed on peritoneal macrophages. The total activity of the respiratory chain enzymes in the NBT-test and superoxide dismutase was determined. Cells of intact animals served as controls. As a positive control, a commercial antigenic LPS preparation Escherichia coli was used. The content of cyclic nucleotides in homogenates of immunocompetent organs was determined using ELISA.Results.This study presents materials on the study of the effect of TE on the bactericidal activity of phagocytes and the level of cyclic nucleotides in immunocompetent organs. It has been established that TEs activate oxygen-dependent bactericidal systems of phagocytes. When studying the effect of TE on the content of cyclic nucleotides in immunocompetent organs of white mice, an increase in their concentration was revealed, indicating an increase in the functional activity of the cells.Conclusion.The obtained data make it possible to substantiate the need for a further detailed study of the immunogenic properties of B. abortus TE in the L- or S-form on the organism of experimental animals

Influence of Bacillus anthracis 34F₂ Sterne Antigen Preparations in Combination with Cobaltarabinogalactan on the Subpopulation Structure of B-lymphocytes (Communication 2)

Study of Bacillus anthracis 34F2 Sterne antigenic preparation S-2 and its combined use with nanostructured cobalt-arabinogalaktan (Со-АG) is presented. The ability of these preparations to stimulate proliferation and differentiation of B-lymphocytes is demonstrated. However, content of the B-lymphocyte circulating subpopulations depends on the time of observation. Co-AG exhibits adjuvant properties enhanced the immunogenic features of the S-2 B. anthracis 34F2 Sterne that may indicate its availability as an adjuvant in the construction of chemical vaccines

Evidence for evolution of growth media in superdeep diamonds from Sao-Luis (Brasil)

Diamonds from Sao-Luis (Brazil) are known to be originated from the depths of transition zone and lower mantle [1]. In this study we consider some aspects of the composition and evolution of growth media for diamonds from this locality. CL imaging has revealed the complex growth history for most diamonds, reflecting their formation in several stages. Nitrogen content in an individual diamonds varies from several to 500 ppm. An apparent tendency for the 3107 cm (super -) (super 1) peak intensity to increase with increasing the nitrogen content gives support to the idea that the conditions favouring the incorporation of nitrogen in these diamonds might also favour the incorporation of hydrogen. Specific feature of diamonds from Sao-Luis is extremely high nitrogen aggregation state (90-100 ±). The set of luminescence centers N3, H3, H4, 490.7 is typical for all diamonds. Radiation-induced centers with peaks at 536 and 576 nm are often observed. The total range of carbon isotope composition in diamonds studied by SIMS makes up from -3.3 to -20.3 ppm of d (super 13) C. Some diamonds show local variations of d (super 13) C between different growth zones (up to 7 ppm). The dominant inclusions in studied diamonds are CaSi-perovskite and AlSi-phases. MgSi- and CaTi-perovskites, ferropericlase, native iron, coesite and zircon have also been found. Raman shift of coesite peak show high residual pressure (>3 GPa at ambient temperature). FTIR study of some microinclusion-bearing diamonds showed that water and carbonates are not major components of diamond-forming fluids. LA-ICPMS bulk composition has significant enrichment in Ca, Fe and Al and strong depletion in Mg. Trace elements show general enrichment in Ti and V and depletion in Sr, LREE and Ni. Most probable source for such environments might be fluids from deeply subducted rocks of metasomatized oceanic lithosphere.1 page(s