Видове різноманіття та особливості поширення дереворуйнівних грибів у парку культури і відпочинку імені Михайла Чекмана міста Хмельницького

The results of studies of the species composition of wood-destroying fungus in the Park of Recreation and Leisure named after Mykhailo Chekman in the city of Khmelnytskyi are presented here. In the Park, 29 species of wood-destroying fungus were found, which are widespread on 14 tree species. The most common types of destroying fungus were: Fomitopsis pinicola, Bjercandera adusta, Bjercandera fumosa, Phellinus igniarius, and Stereum hirsutum. These types of wood-destroying fungus are found on almost all tree species of the park which have been damaged by the fungi. The tree species, which are most susceptible to wood-destroying fungus, are: Betula pendula Roth., Fraxinus excelsior L., Salix alba L., Populus nigra and Quercus robur L. The lowest occurance of wood-destroying fungi was found on вільсі чорній and грабові звичайному. It has been proved that the role of wood-destroying fungus in urban plantations is negative. Most species of wood-destroying fungus cause a variety of rot. Such trees have no external signs of weakening for a long time. It is possible to find affected trees with wood-destroying mushrooms by the presence of fruit bodies. The methods of dealing with wood-destroying fungus are presented here. The main method is conducting periodic forest-pathological surveys to detect the species composition of wood-destroying fungus and the degree of affliction of plantations. In the park, along with the implementation of sanitary measures, the individual protection of trees is important. Methods of protection are; wound healing, wound rubbing after pruning and filling of tree hollows. Protection of trees on the basis of chemical methods should be carried out with single defeat. Measures to regulate recreational loads and prevent mechanical and other tree damage are also important in Chekman Park. The obtained research results broaden the idea of the species diversity of wood-destroying fungi in the park plantations of Khmelnytskyi and can be used in the organization and implementation of forest-pathological monitoring, as well as determining the locations of future sanitary and recreational activities.Наведено результати дослідження видового складу дереворуйнівних грибів у Парку культури і відпочинку ім. Михайла Чекмана міста Хмельницького та біоекологічні особливості їх поширення. У Парку культури і відпочинку ім. Михайла Чекмана виявлено 29 видів дереворуйнівних грибів, які поширені на 14 видах деревних порід. Найпоширенішими виявилися таки види дереворуйнівних грибів: трутовик облямований, б'єркандера обвуглена, б'єркандера димчаста, трутовик несправжній та стереум жорстковолосистий. Ці види дереворуйнівних грибів трапляються майже на всіх деревних видах парку, які пошкоджені дереворуйнівними грибами. Ці гриби трапляються поодиноко на видах дерев I та ІІ стадій деструкції, дуже рідко – на III стадії. Встановлено деревні породи, які найбільше піддаються ураженню дереворуйнівними грибами, це такі види: береза повисла, ясен звичайний, верба біла, тополя чорна та дуб звичайний. Доведено, що роль дереворуйнівних грибів у міських насадженнях негативна. Більшість видів дереворуйнівних грибів спричиняють різноманітну гниль. Такі дерева довгий час не мають зовнішніх ознак ослаблення. Встановити вражені дерева дереворуйнівними грибами можна тільки за наявністю плодових тіл. Запропоновано основні методи боротьби з дереворуйнівними грибами у парку

The minimal compound-complex sentence hierarchy

This article deals with the problem of the study of the subsystem of such type of the Multiple Compound-Complex Sentence as a minimal compound-complex sentence. The authors analyze the formal structure of the multiple compoundcomplex sentences and try to build up the structural models hierarchy of Minimal Compound-Complex SentenceyesBelgorod State Universit

Synthesis, crystal structure and hydrolysis of novel isomeric cage (P-C/P-O)-phosphoranes on the basis of 4,4,5,5-tetramethyl-2-(2-oxo-1,2-diphenylethoxy)-1,3,2-dioxaphospholane and hexafluoroacetone

© The Royal Society of Chemistry 2016.The reaction of 4,4,5,5-tetramethyl-2-(2-oxo-1,2-diphenylethoxy)-1,3,2-dioxaphospholane with hexafluoroacetone leads to the simultaneous formation of regioisomeric cage (P-C/P-O)-phosphoranes, the structures of which are unequivocally confirmed by XRD. The rearrangement of the P-C-isomer to P-O-isomer with high stereoselectivity (>96%) takes place in methylene chloride solution with the retention of the phosphorus coordination. It was found that the stepwise hydrolysis of the P-O-isomer initially gives 2-(2,3-dihydroxy-1,2-diphenyl-3-trifluoromethyl-4,4,4-trifluorobutyloxy)-4,4,5,5-tetramethyl-2-oxo-1,3,2-dioxaphospholane as the only stereoisomer whose structure is also confirmed by XRD. Further hydrolysis of this compound leads to the formation of 2,3-dihydroxy-3-trifluoromethyl-4,4,4-trifluoro-1,2-diphenylbutylphosphate and pinacol, which forms the solvate in the crystal. Hydrolysis of the P-C-isomer yields 2-hydroxy-4,4,5,5-tetramethyl-2-oxo-1,3,2-dioxaphospholane, benzoin and hexafluoroisopropanol

Modern genetic and immunological aspects of the pathogenesis of impaired consolidation of fractures (literature review)

The aim of this article is to analyze the genetic and immunological mechanisms of the development of fracture consolidation disorders at the present scientific stage.Materials and methods. The search for literary sources was carried out in the open electronic databases of scientific literature PubMed and eLIBRARY. Search depth – 10 years.Results. The review analyzes the literature data on the current state of the study of the molecular genetic mechanisms of reparative regeneration including the development of fracture consolidation disorders. The mechanisms of the most important links of pathogenesis which most often lead to various violations of the processes of bone tissue repair are considered.Conclusion. The process of bone tissue repair is multifaceted, and many factors are involved in its implementation, however, we would like to note that the leading role in the course of reparative regeneration is played by a personalized genetically programmed response to this pathological condition. Nevertheless, despite the undeniable progress of modern medicine in studying the processes of bone recovery after a fracture, there are still many “white” spots in this issue, which dictates the need for further comprehensive study in order to effectively treat patients with impaired consolidation

Assessment of pediatric faculty students 'satisfaction with distance educational process at the department of hospital pediatrics under COVID19 pandemic conditions

The article presents the results of a questionnaire survey of 5-6-year students in the discipline "Hospital Pediatrics" and 5-year students in the discipline "Evidence-Based Medicine" in the context of the Covid19 pandemic in order to assess student satisfaction with the distance learning process. The survey was conducted at the end of the study of these disciplines. The results of the survey showed that the students are satisfied with the quality of the organization of the distance educational process at the Department of Hospital PediatricsВ статье представлены результаты анкетного опроса студентов 5-6 курсов по дисциплине «Госпитальная педиатрия» и студентов 5 курса по дисциплине «Основы доказательной медицины» в условиях пандемии COVID-19 с целью оценки удовлетворенности студентов дистанционным образовательным процессом. Анкетирование проведено по окончании изучения данных дисциплин. Результаты анкетирования показали, что обучающиеся удовлетворены качеством организации дистанционного образовательного процесса на кафедре Госпитальной педиатри

Enhancement of the Technology for Live Tularemia Vaccine Production

Objective of the study was to develop and test new biotechnological approaches for live tularemia vaccine production.Materials and methods: Francisella tularensis 15 NIIEG strain was used as producer-strain; Francisella tularensis 503 strain – as test infecting one. Producer strain was cultivated on solid and liquid nutrient media. Tangential ultrafiltration was performed with the help of microfiltration module “Viva-flow”. Lyophilization was conducted using drying installation – Free Zone 2.5 L.Results and discussion: Application of the designed liquid nutrient medium on the basis of enzymatic fibrin hydrolysate and submerged cultivation of the producer-strain has allowed for a significant biomass yield increment. At the stage of tularemia microbe culture concentration via microfiltration through filtering membranes with pore size of 0.2 μm, in the mode of tangential liquid flow, increased has been the content of microbe cells; the nutrient media residues – removed. Comparative analysis of the obtained in accordance with experimental technique laboratory series of the vaccine and commercial preparation of live tularemia vaccine has demonstrated their conformity with the specific normative properties. It is established that application of modified liquid nutrient medium, submerged cultivation conditions, methods of biomass concentration and separation has no negative influence on the main properties of live tularemia vaccine and will provide for considerable produce-ability increase in the future

Effectiveness of the “Sterius 60” SHF Radiation Installation for Disinfection of Objects Contaminated with PBA of Groups I–IV, when Working with Infected Biomodels

The aim was to evaluate the effectiveness of using the “Sterius 60” microwave disinfection system (Russia) for decontamination of objects infected with PBA of groups I–IV emerging as a result of working with infected laboratory animals.Materials and methods. Effectiveness verification of disinfection of biological waste generated as a result of the life of laboratory animals by SHF radiation was carried out in the microwave system “Sterius 60”, recommended by the manufacturer for disinfection of epidemiologically hazardous and extremely dangerous medical waste, including biological ones (classes B and C), by volumetric SHF heating. Carcasses of uninfected laboratory animals (white mice, Guinea pigs, suckling rabbits), granulated feed and bedding material (wood shavings), which are objects directly in contact with biomodels, were used as vivarium waste to be decontaminated. The following microorganisms were utilized as model test ones: Bacillus subtilus VKM B-911, Bacillus stearothermophilus VKM B-718, Bacillus licheniformis G VKM B-1711-D, Alcaligenes faecalis 415, Yersinia pestis EV, Bacillus anthracis STI. Laboratory utensils (plastic Petri dishes, porcelain mortars and pestles) were used as a mock-up chamber filler for model test microorganisms.Results and discussion. As a result of the study, data were obtained indicating that the microwave system for disinfection of medical waste “Sterius 60” is ineffective for decontamination of biological waste in laboratories working with biomodels infected with PBA of groups I–II. The established standard mode of disinfection of this system was effective only for non-spore forms of microorganisms, pathogenicity groups III–IV. Therefore, in our opinion, it is advisable to use it for decontamination of laboratory utensils infected with PBA of groups III–IV, directly at sites of waste generation

Population Genomics of SARS-CoV-2 in the Constituent Entities of Siberian and Far Eastern Federal Districts

The aim of the study was to analyze the genetic structure of populations and the patterns of evolutionary variability of the novel coronavirus infection in the Siberian and Far Eastern Federal Districts. Materials and methods. 1033 SARS-CoV-2 genomes from samples from individuals diagnosed with COVID-19 from eight entities of the Siberia and Far East between December 2020 and November 2021 were assessed. Sequencing was performed on the MinION Oxford Nanopore platform using the ARTIC v.3 protocol. The degree of SARS-CoV-2 genetic isolation was estimated applying the Fst criterion. Phylogenetic analysis was carried out using maximum likelihood method and Bayesian phylogenetic inference. A nonparametric Bayesian Skyline Plot (BSP) model was used to reconstruct population dynamics. Results and discussion. The original SARS-CoV-2 variant (B.1) was identified in 100 % of the cases at the initial stages. The Alpha variant was detected in March-June, 2021; Beta – in single samples in March-May, 2021. Delta was first identified in April, 2021. The maximum degree of SARS-CoV-2 genetic isolation (Fst=0.18) was established for the most remote territories (Altai Territory ↔ Republic of Buryatia and Altai Territory ↔ Irkutsk Region). A relatively free circulation of the virus was detected between Irkutsk Region, Republic of Buryatia and Krasnoyarsk Territory. According to the results of population genetic tests, a sharp increase in the effective virus population size was the determining mechanism of SARS-CoV-2 genetic diversity formation. Reconstruction of population dynamics in BEAST (BSP model) has revealed the consistency of trends in the genetic diversity of the virus and the number of active cases. Two subclusters have been identified in the Delta cluster, consisting predominantly of samples isolated in the Irkutsk Region and Krasnoyarsk Territory. Change in the dominant variant of SARS-CoV-2 has been traced in dynamics. Molecular-epidemiological data point to the multiple pathways of spatial expansion of different SARS-CoV-2 genotypes into the constituent entities with generation of individual monophyletic clusters and further intra- and extraterritorial spread of the decedents