Nanosized magnetite modified with poly(ethylene glycol) for efficient sorption of L-lysine-α-oxidase from the culture fluid

Fe3O4@PEG have been proposed for sorption of L-lysine-α-oxidase (LO) from the culture fluid of Trichoderma harzianum Rifai F-180 for the first time. To synthesize the Fe3O4@PEG nanoparticles, an original method based on aqueous biphasic systems has been developed. The PEG-modified magnetite provide a high sorption ability towards LO in contrast to the non-modified Fe3O4 synthesized by the traditional precipitation method. The morphology and structure of the prepared nanoparticles were characterized by TEM, FTIR and XRD. The data on magnetic properties and stability in physiological media are presented. The synthesized nanoparticles ensure quantitative sorption and desorption of LO during at least 3 cycles. © 2022 Elsevier B.V