PERSONALITY TYPE D AND THE LEVEL OF SUBCLINICAL INFLAMMATION MARKERS IN CHD PATIENTS

Aim. To study the level of biomarkers of subclinical inflammation in CHD patients with existence of absence of personality type D.Material and methods. In 62 patients with stable CHD before planned CBG operation we assessed concentrations: of matrix metalloproteases -2, -3, -9, tissue inhibitor of matrix proteases -1, -2, C-reactive protein, soluble CD40 ligand, tumor necrosis factor alpha. For further study we selected two groups: 1 group (n=27) — patients with type D and 2 group (n=35) — with non-D type. Personality type was defined with DS-14 questionnaire. Patients were asked 14 questions with 5 variants of responses to each. Counting of points was done by two scales: negative irritability and social suppression. Type D was set if 10 points and more by each of the scales. All patients underwent: general and biochemical blood tests, echocardiography, color duplex scanning of brachiocephalic arteries and coronary angiography.Results. In intergroup comparison both groups were comparable by the most anamnestic and clinical parameters. There were no any differences in the instrumental assessments data. In type D patients there was higher level of MMP-9 (resp. 53,2 pg/mL and 66,5 pg/mL; р=0,051) and sCD40L (resp. 2,8 ng/mL and 5,7 ng/mL; р=0,013) comparing with those non-D-type. The level of other biomarkers in groups did not differ, concentration of MMP-2 and TNF-α were slightly higher among type-D, of the others — among non-D-typers. There were also statistically significant negative correlations of type-D and MMP-9 and sCD40L.Conclusion. In CHD patients, examined before coronary bypass operation, presence of type D personality was not followed by the increase of subclinical inflammation markers level comparing to patients without such type. In type-D patients there was increase of platelet activation markers activation, that associates with adverse outcomes in CHD

Nanoparticles for Applications in Cellular Imaging

In the following review we discuss several types of nanoparticles (such as TiO2, quantum dots, and gold nanoparticles) and their impact on the ability to image biological components in fixed cells. The review also discusses factors influencing nanoparticle imaging and uptake in live cells in vitro. Due to their unique size-dependent properties nanoparticles offer numerous advantages over traditional dyes and proteins. For example, the photostability, narrow emission peak, and ability to rationally modify both the size and surface chemistry of Quantum Dots allow for simultaneous analyses of multiple targets within the same cell. On the other hand, the surface characteristics of nanometer sized TiO2allow efficient conjugation to nucleic acids which enables their retention in specific subcellular compartments. We discuss cellular uptake mechanisms for the internalization of nanoparticles and studies showing the influence of nanoparticle size and charge and the cell type targeted on nanoparticle uptake. The predominant nanoparticle uptake mechanisms include clathrin-dependent mechanisms, macropinocytosis, and phagocytosis

FACTORS INFLUENCING ON THE MORTALITY IN PATIENTS WITH OBLITERATING DISEASES OF LOWER LIMB ARTERIES

Aim. To study the risk factors associated with the fatal outcome in patients with obliterating diseases of the lower extremities arteries at the stage of outpatient observation.Material and methods. The study included 467 patients (381 men and 86 women, the median age – 63 years [59;70]) with obliterating atherosclerosis of lower limb arteries >50%. Patients were examined in the framework of the current registry on the basis of the Kemerovo Cardiology Center for the period from 2009 to 2013. The examination included the dynamic observation of an angiosurgeon and a cardiologist, the determination of instrumental and laboratory indicators. During 3 years of follow-up, 42 deaths were registered (8.99% of the total number of patients), including 28 (65%) ones due to cardiovascular diseases. Patients were divided into two groups – with a fatal outcome (9%) and without it (91%).Results. The lethal outcome was associated with the male gender (83%), disability (69.8%), retirement status (85.5%), higher incidence of previously diagnosed myocardial infarction (38.5%), angina pectoris (66.6%), stroke or transient ischemic attack (19%). Lesions of two (86%) and three arterial basins (36%) and significant coronary bed lesions (74%) were more frequent in patients with lethal outcome, they were characterized by a longer smoking experience (17.9 years) and hypertension (14.5 years). Surviving patients were more likely to undergo reconstructive surgeries on lower limb arteries (26.5%).Conclusion. Factors associated with death in patients with obliterating diseases of the lower extremities arteries were angina pectoris [odds ratio (OR) 1.14, 95% confidence interval (CI) 1.04-1.43; p=0.041), smoking experience (OR 1.28, 95% CI 1, 8-3,24; p=0,023), the duration of hypercholesterolemia (OR 2.01, 95% CI 0.35-4.12; p=0.012) and intermittent claudication (OR 2.01, 95% CI 0.35-4.12; p=0.041), presence of coronary artery stenosis ≥50% (OR 3.21, 95% CI 2.02-6.14; p=0.031), atherosclerosis of two or more arterial basins ≥50% (OR 3.43, 95% CI 3.46-5.52; p=0.004), the presence of a history of myocardial infarction (OR 2.12, 95% CI 0.75-6.02; p=0.043), and stroke (OR 2.23, 95% CI 2.05-4.21; p=0.021). To improve the effectiveness of secondary prevention in these patients it is advisable to use new organizational approaches at the outpatient stage of their management

Microwave-assisted growth and characterization of water-dispersed CdTe/CdS core-shell nanocrystals with high photoluminescence

Journal of Physical Chemistry B1102713370-13374JPCB