Hormonal characteristics of androgen status and their relationship with the anthropometric and metabolic parameter in men depending on the severity of hypertension

Background. According to the literature data, long-term and poorly controlled hypertension (HTN) leads to target organs damage, and in some cases, to the development of associated diseases, the severity of which in men may be partly due to the characteristics of the androgenic status.The aim: to reveal the features of hormonal indicators of androgen status and their relationship with the anthropometric and metabolic characteristics of the therapeutic clinic male patients, depending on the severity of HTN.Materials and methods. 296 men aged 59,0 [54,0; 65,0] years with confirmed HTN were examined. All study participants in the morning on an empty stomach underwent anthropometry, determination of metabolic and androgenic status, and assessment of comorbid burden. Depending on the severity of hypertension, all patients were divided into two groups (G): G1 (n = 134) – patients with hypertension without a history of coronary artery disease or stroke; G2 (n = 162) – patients with hypertension with a history of ischemic heart disease (IHD) and/or stroke.Results. In male patients of G2 with severe HTN, a decrease in the levels of free testosterone (FT) and, to a greater extent, dehydroepiandrosterone sulfate (DHEA-S), as well as total cholesterol, high and low density lipoproteins, was revealed. The correlation analysis performed revealed statistically significant negative correlations between the levels of total testosterone (TT) and FT with body mass index, the value of the ratio of waist circumference to hips, only the level of FT – with the presence of IHD, chronic heart failure (CHF), disorders of carbohydrate metabolism and abdominal type of fat distribution, and the level of DHEA-S – with the presence of IHD and CHF.Conclusion. A decrease in FT and DHEA-S is associated with early progression of atherosclerosis and the risk of developing HTN-associated cardiovascular events, which can aggravate the course of HTN

BIOINFORMATIC SEARCH OF CRISPR/CAS SYSTEM STRUCTURES IN GENOME OF PCT281 PLASMID OF BACILLUS THURINGIENSIS SUBSP. CHINENSIS STRAIN CT-43

Background. CRISPR/Cas systems loci are one of the functionally important patterns in bacterial genome which perform the role of “adaptive immune defense” from foreign nucleic acids. The study of CRISPR/Cas systems structure in genomes of plasmids and phages provide new information about the evolution of this systems in bacterial hosts.Aims. A search of CRISPR/Cas systems structures in pCT281 plasmid from Bacillus thuringiensis subsp. chinensis strain CT-43 using bioinformatic methods.Materials and methods. Search studies using bioinformatics methods were performed with the genome of pCT281 plasmid of B. thuringiensis subsp. chinensis strain CT-43 from the RefSeq database. To search for the CRISPR/Cas system structure MacSyFinder (ver. 1.0.5) and three combined algorithms were used: CRISPRFinder; PILER-CR; CRISPR Recognition Tool (CRT). The consensus repeat sequence was generated in WebLogo 3.Results and discussion. In pCT281 plasmid we detected one locus of CRISPR/Cas system of the type I-C which contains 2 CRISPR-cassettes and 4 cas-genes located between them. The CRISPR-cassette 1 includes 10 spacers from 32 to 35 bp and 11 repeats 32bp in length. 5 spacers (33–35 bp) separated by 6 repeats 32 bp in length were detected in the CRISPR-cassette 2.Conclusions. The bioinformatic methods used in this study enable to conduct a search of CRISPR/Cas systems structures in plasmid genomes. The presence of the CRISPR-Cas locus in pCT281 plasmid confirms a possible transfer of this system from the nucleoid to this plasmid. The detected spacers provide information about phages this bacteria was encountered

BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA

The emergence of resistance among the most important bacterial pathogens is generally recognized as one of the major public health problems. The most important of these organisms are penicillin-resistant Streptococcus pneumoniae, vancomycin-resistant enterococci and methicillin- and vancomycin-resistant Staphylococcus aureus. These antibiotic resistance in common pathogens have made antimicrobial therapy of many infections. Scientists need to look for new ways of treating bacterial infections in the work, using the developed algorithm from the methods of search software in the genomic structure of Staphylococcus aureus subsp. aureus ST228, the CRISPR/Cas locus and the division structures of its CRISPR cassette. The results of the bacteriophage search through the decoded spacer sequences of CRISPR-cassettes of this strain were also obtained using the developed algorithm of the software methods of bioinformatics. It was determined that the CRISPR/Cas system of strain of ST228 of S. aureus was of type IIIA. It is shown that cas-genes are in the immediate vicinity of CRISPR cassettes. The spacer structures in the detected CRISPR cassette are the Staphylococcus, Mycobacterium, Streptococcus, Bacillus, Gordonia, Arthrobacter, Streptomyces. The implementation of the algorithm of program methods for locating CRISPR/Cas-loci can be applied to many other decoded bacterial genomes to return bacteriophage therapy

Financial Strategy of Enterprises: Formulation and Results over 2000—2009 (the Khabarovsk Territory’s example)

The article considers the concepts of capital and capital management strategies in the non-financial economic sector, as well as the need for analytical studies in this area in terms of strategic interests of the regional economy. Monitoring of enterprises as a methodological basis for analytical studies of the regional economy and financial strategies of enterprises is conducted. Factors that shape the differences in financial strategies at the level of enterprise groups of certain types and range of activity are identified. The efficiency of strategies and their impact on the regional economy as a whole is state

A Clinical Case of Out-of-Hospital Cardiac Arrest and Post-Resuscitation Disease in a Patient with Acute Myocardial Infarction

Background. Prediction of survival in adult patients after out-of-hospital cardiopulmonary resuscitation remains a difficult issue. This clinical case describes our experience of managing a patient after an out-of-hospital cardiac arrest due to an acute coronary syndrome with the development of postanoxic coma, with subsequent successful restoration of neurological function. Case description. Patient K., a 48-year-old man, was delivered unconscious (Glasgow Coma Scale score 7) without spontaneous breathing. During transportation to the hospital, ventricular fibrillation occurred, cardiopulmonary resuscitation lasting 1 hour was performed with restoration of sinus rhythm. Diagnosis: acute coronary syndrome with ST segment elevation; cardiogenic shock; condition after cardiopulmonary resuscitation. Stenting of the occluded circumflex artery was performed. During the patient’s stay in the intensive care unit (ICU), hemodynamics and laboratory tests were carefully monitored and no significant systemic disorders were detected. The patient stayed in the ICU for 19 days. At the time of discharge, the general condition was relatively satisfactory, Glasgow Coma Scale score was 14-15, with minimal neurological deficit. The patient was transferred to a neurological department for further rehabilitation. Results. Determining the probability of either good or bad outcome in comatose patients after cardiac arrest is one of the most important steps in treatment after stabilization of the heart activity. Recent data confirm the utility of electroencephalography (EEG) monitoring for prognosis. The pattern of response to sedative drugs may provide prognostic information. In this clinical case, when EEG was recorded during sedation, preservation of the response to stimuli was registered, and 48 hours after disconnection, patterns corresponding to awakening were registered, which was considered as a positive prognostic sign. Conclusions. In adult patients after cardiopulmonary resuscitation with postanoxic coma, clinical, neurophysiological and radiological tests predict poor neurologic outcome within the first week after cardiac arrest with a certain percentage of false positive rate. However, most predictors have low sensitivity and specificity. Accurate prognosing requires a comprehensive approach. Neurological examination remains the gold standard, but its result is affected by the effect of sedative drugs used in critical patients in the ICU. The use of EEG increases the accuracy of the prognosing

Anti-MOG associated myelitis

There is now increasing evidence that demyelinating disease with anti-myelin oligodendrocyte glycoprotein (anti-MOG) antibodies is an independent nosological unit. The paper describes a clinical case of anti-MOG associated myelitis at the CI-TXlevel. Differential diagnosis was made between multiple sclerosis, Devic's myelitis optica, and idiopathic transverse myelitis. The clinical, morphopathological, and diagnostic features of anti-MOG associated myelitis are discussed. There are new diagnostic criteria for neuromyelitis optica spectrum diseases (NMOSD), as well as red flags, in the absence of which the diagnosis of NMOSD can be established as a diagnosis of exclusion

PSYCHOPHYSIOLOGICAL CHARACTERISTICS OF WOMEN IN DEPENDENCE ON THE OVARIOHORMONAL CYCLE PHASE AND PROGESTERONE ACTIVITY (PART 2)

With the aim to identify the effects of menstrual cycle phase on the alpha EEG characteristics the 78 women aged 18–27 years were studied in a within-subject design Half the subjects began investigation at their follicular phase and half at their luteal phase (LP). The alpha peak frequency, alpha band width and power inalpha-2 range are highest, but power in alpha 1 and activation are lowest in LP that is associated with the highest saliva progesterone level

Back pain: from diagnosis to treatment

In today ‘s world, back pain is a heavy burden and leads to a decrease in the working capacity, quality of life of people. The choice of tactics of treatment of pain in a back is defined by the pain reason: nonspecific pain, the specific pain caused by serious diseases or a compression of a root. The most frequent cause of back pain is nonspecific pain due to myofascial, muscular-tonic syndrome, facet syndrome, and sacral-iliac joint dysfunction in lower back pain. The article presents clinical symptoms of these syndrome, as well as techniques of neuroorthopedic examination, which allow to detect one or more abrasions of back pain. To prevent acute non-specific pain, it is recommended to prescribe non-steroidal anti-inflammatory drugs (NSAID) and muscle relaxants. Along with pharmacological treatments, treatment of patients with back pain should be more comprehensive and include cognitive-behavioral therapy and kinesiotherapy. Determination of the source of back pain in neuroorthopedic examination makes it possible to carry out local therapy in stages using blockages with local anesthetics and glucocorticoids. In case of insufficient effectiveness of blockades, it is possible to carry out radiofrequency denervation of facet joints or sacral-iliac). Clinical and neuroorthopedic examination of a patient with back pain with identification of sources of pain, analysis of the causes that led to its development, adequate treatment and recommendations to prevent repeated exacerbations can significantly reduce the risk of chronization of back pain and improve the quality of life of patients

Treatment of patients with acute and subacute lumbodynia and lumbar ischialgia

No effect of outpatient treatment is observed in some patients with acute nonspecific lumbodynia and lumbar ischialgia; therefore they are referred to pain management departments or centers for specialized examination and treatment.Objective: to study of the anatomical causes of pain and the efficiency of celecoxib (Dilaxa) treatment in patients with acute and subacute lumbodynia and lumbar ischialgia in the inpatient setting.Patients and methods. Eighty patients (47 women and 33 men; mean age, 51.0±15.3 years) with acute and subacute lumbodynia (n=11) or lumbar ischialgia (n=69) were followed up. Pain intensity was assessed using a visual analogue scale (VAS); functional limitation was rated according to the Roland–Morris scale (RMS). To reduce the intensity of pain syndrome, patients took oral celecoxib (Dilaxa) 200 mg twice daily. Therapeutic blockades with anesthetics and glucocorticoids were used in some cases of damage to the sacroiliac joint (SIJ) or facet joints (FJ). Patients were informed of the benign nature of the disease; they did therapeutic exercises.Results and discussion. The causes of pain were ascertained to be injuries of SIJ (48,8%), FJ and SIJ (18,8%), FJ (17,5%), herniated disc (10%), piriformis syndrome (2.5%), and piriformis syndrome and SIJ injury (2.5%). The duration of treatment averaged 11.0±2.4 days. By the end of the study, there was a clinically significant reduction in pain in 86% of patients. The treatment resulted in a 2.4-fold average reduction in pain intensity (from 7.0 to 2.9 VAS scores): a 4-fold reduction in patients with lumbodynia (from 6.4 to 1.6 scores) and a 2.3-fold one in those with lumbar ischialgia (from 7.1 to 3.1. scores); the degree of disability reduced by an average of 1.9 times (from 8.1 to 4.3 RMS scores): by 3.6 times (from 6.8 to 1.9 scores) and 1.9 (from 8,3 to 4,7 scores) times in patients with lumbodynia and in those with lumbar ischialgia, respectively. No adverse events were observed during the treatment period.Conclusion. There were high SIJ and FJ injury rates in subacute lumbar ischialgia. Celecoxib was noted to be highly effective and safe in treating acute and subacute lumbar ischialgia and lumbodynia