Antioxidant defense system state in blood plasma and heart muscle of rats under the influence of histamine and sodium hypoclorite

There is a wide spectrum of antihistamine drugs in the pharmaceutical market, however all these chemical preparations cause side effects. Therefore, new alternative ways for histamine detoxication are to be found. For this aim in our experiment sodium hypochlorite was used because its solution possesses strong oxidizing properties. The influence of histamine and sodium hypochlorite on the antioxidant defence system state of blood plasma and cardiac muscle in rats has been researched. It was shown, that the investigated factors result in the disruption of the antioxidant system. It was found that histamine injection in concentration of 1 and 8 μg/kg in plasma leads to the increase of superoxi­de dismutase activity during all the experiment. When studying enzymes, that catalyze hydroperoxides and Н2О2 decomposition it was shown that under the influence of histamine in a dose 1 μg/kg, the glutathione peroxidase activity increased on the 1st day of the experiment. However, on the 7th day of the experiment the increase of both glutathione peroxidase and catalase activity was fixed. The deviation in superoxide dismutase function in rats plasma under the action of sodium hypochlorite has been established. The activity of enzymes that decompose Н2О2 and hydroperoxides were inhibi­ted. Under the influence of histamine in the heart tissues we have stated the disturbance of superoxide dismutase work and increase of catalase activity and decrease of glutathione peroxidase activity. The influence of sodium hypochlorite on the myocardium of intact animals as well as joint influence of sodium hypochlorite and histamine result in the increase of superoxide dismutase and catalase activity and lead to the conside­rable decline of activity of glutathione peroxidase

Effects of histamine and sodium hypochlorite on free radical processes in lung tissues of rats

Influence of histamine and sodium hypochlorite on the processes of lipid peroxidation and status of antioxidant defence system of rats lungs was estimated. We found that after exogenous injection of histamine and sodium hypochlorite, content of the endogenous histamine increased during the experiment. It was shown that applied chemicals led to overaccumulation of lipid peroxidation products and disfunction in the antioxidant enzymes. During studying the activities of antioxidant enzymes of lung homogenates, it was found that the activity of superoxide dismutase increased on the 1st day of experiment under influence of histamine (1 µg/kg and 8 µg/kg). However, after 21 days of experiment its activity decreased and reached control values. Under the action of histamine and sodium hypochlorite in concentration of 1 µg/kg, superoxide dismutase activity increased. At the 7th day of experiment, its activity increased. High concentration of histamine and the action of sodium hypochlorite in lung tissues of rats caused a significant activation of superoxide dismutase on the 1st day of experiment, and this index decreased and became lower than control one at the 7th and 14th days. Under the action of histamine and sodium hypochlorite, catalase activity increased compared to control indices. At the same time, glutathione peroxidase activity increased on the 7th day of experiment with its further decrease

Activity of key enzymes of antioxidant system in rat blood plasma under the effect of histamine and sodium hypochlorite

The effects of histamine in 1 mg/kg and 8 mg/kg doses that correspond to the doses causing pathological effects at experimental conditions and of sodium hypochlorite in 5 mg/l dose – the lowest concentration of sodium hypochlorite that affects a body by oral administration, on the key enzymes of blood plasma antioxidant system were studied. It was found that histamine used in both concentrations intensified superoxide dismutase activity for 14 days. The simultaneous injections of histamine and sodium hypochlorite caused significant activation of superoxide dismutase in rats. Sodium hypochlorite received by rats with drinking solution caused the same effect. The catalase activity of blood plasma was not significantly affected by histamine, and its activity was significantly increased only under the influence of biogenic amine in 1 mg/kg dose on the 7th day of the experiment. Sodium hypochlorite caused a decline in catalase activity both in intact animals and in animals that received histamine injections subcutaneously. The injection of histamine in 1 mg/kg dose caused an increase in glutathione peroxidase activity on the 1st and 7th day of the experiment. Histamine in 8 mg/kg dose caused the intensification of glutathione peroxidase activity only on the 1st day, followed by the inhibition on the 14th day of the experiment. Sodium hypochlorite received by rats with drin­king solution led to general lowering of glutathione peroxydase activity in blood plasma