Influence of high gas production during thermophilic anaerobic digestion in pilot-scale and lab-scale reactors on survival of the thermotolerant pathogens Clostridium perfringens and Campylobacter jejuni in piggery wastewater

Safe reuse of animal wastes to capture energy and nutrients, through anaerobic digestion processes, is becoming an increasingly desirable solution to environmental pollution. Pathogen decay is the most important safety consideration and is in general, improved at elevated temperatures and longer hydraulic residence times. During routine sampling to assess pathogen decay in thermophilic digestion, an inversely proportional relationship between levels of Clostridium perfringens and gas production was observed. Further samples were collected from pilot-scale, bench-scale thermophilic reactors and batch scale vials to assess whether gas production (predominantly methane) could be a useful indicator of decay of the thermotolerant pathogens C. perfringens and Campylobacter jejuni. Pathogen levels did appear to be lower where gas production and levels of methanogens were higher. This was evident at each operating temperature (50, 57, 65 °C) in the pilot-scale thermophilic digesters, although higher temperatures also reduced the numbers of pathogens detected. When methane production was higher, either when feed rate was increased, or pH was lowered from 8.2 (piggery wastewater) to 6.5, lower numbers of pathogens were detected. Although a number of related factors are known to influence the amount and rate of methane production, it may be a useful indicator of the removal of the pathogens C. perfringens and C. jejuni

Exercise training-induced PPARβ increases PGC-1α protein stability and improves insulin-induced glucose uptake in rodent muscles

This study aimed to investigate the long-term effects of training intervention and resting on protein expression and stability of peroxisome proliferator-activated receptor β/δ (PPARβ), peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC1α), glucose transporter type 4 (GLUT4), and mitochondrial proteins, and determine whether glucose homeostasis can be regulated through stable expression of these proteins after training. Rats swam daily for 3, 6, 9, 14, or 28 days, and then allowed to rest for 5 days post-training. Protein and mRNA levels were measured in the skeletal muscles of these rats. PPARβ was overexpressed and knocked down in myotubes in the skeletal muscle to investigate the effects of swimming training on various signaling cascades of PGC-1α transcription, insulin signaling, and glucose uptake. Exercise training (Ext) upregulated PPARβ, PGC-1α, GLUT4, and mitochondrial enzymes, including NADH-ubiquinone oxidoreductase (NUO), cytochrome c oxidase subunit I (COX1), citrate synthase (CS), and cytochrome c (Cyto C) in a time-dependent manner and promoted the protein stability of PPARβ, PGC-1α, GLUT4, NUO, CS, and Cyto C, such that they were significantly upregulated 5 days after training cessation. PPARβ overexpression increased the PGC-1α protein levels post-translation and improved insulin-induced signaling responsiveness and glucose uptake. The present results indicate that Ext promotes the protein stability of key mitochondria enzymes GLUT4, PGC-1α, and PPARβ even after Ext cessation

Molecular Dynamics Simulation of Ga Penetration along Grain Boundaries in Al: a Dislocation Climb Mechanism

Many systems where a liquid metal is in contact with a polycrystalline solid exhibit deep liquid grooves where the grain boundary meets the solid-liquid interface. For example, liquid Ga quickly penetrates deep into grain boundaries in Al, leading to intergranular fracture under very small stresses. We report on a series of molecular dynamics simulations of liquid Ga in contact with an Al bicrystal. We identify the mechanism for liquid metal embrittlement, develop a new model for it, and show that is in excellent agreement with both simulation and experimental data

Herschel Survey of the Palomar-Green QSOs at Low Redshift

We investigate the global cold dust properties of 85 nearby (z < 0.5) QSOs, chosen from the Palomar-Green sample of optically luminous quasars. We determine their infrared spectral energy distributions and estimate their rest-frame luminosities by combining Herschel data from 70 to 500 microns with near-infrared and mid-infrared measurements from the Two Micron All Sky Survey (2MASS) and the Wide-Field Infrared Survey Explorer (WISE). In most sources the far-infrared (FIR) emission can be attributed to thermally heated dust. Single temperature modified black body fits to the FIR photometry give an average dust temperature for the sample of 33~K, with a standard deviation of 8~K, and an average dust mass of 7E6 Solar Masses with a standard deviation of 9E6 Solar Masses. Estimates of star-formation that are based on the FIR continuum emission correlate with those based on the 11.3 microns PAH feature, however, the star-formation rates estimated from the FIR continuum are higher than those estimated from the 11.3 microns PAH emission. We attribute this result to a variety of factors including the possible destruction of the PAHs and that, in some sources, a fraction of the FIR originates from dust heated by the active galactic nucleus and by old stars.Comment: accepted for publication in ApJ

The Light Curve and Internal Magnetic Field of the Mode-Switching Pulsar PSR B0943+10

A number of radio pulsars exhibit intriguing mode-switching behavior. Recent observations of PSR B0943+10 revealed correlated radio and X-ray mode switches, providing a new avenue for understanding this class of objects. The large X-ray pulse fraction observed during the radio quiet phase (Q mode) was previously interpreted as a result of changing obscuration of X-rays by dense magnetosphere plasma. We show that the large X-ray pulse fraction can be explained by including the beaming effect of a magnetic atmosphere, while remaining consistent with the dipole field geometry constrained by radio observations. We also explore a more extreme magnetic field configuration, where a magnetic dipole displaced from the center of the star produces two magnetic polar caps of different sizes and magnetic field strengths. These models are currently consistent with data in radio and X-rays and can be tested or constrained by future X-ray observations.Comment: 5 pages, 5 figures, submitted to ApJ