PROLIFERATIVE AND SECRETORY RESPONSE OF MONONUCLEAR LEUCOCYTES TO COMBINED INFLUENCE OF ETHAMBUTOL AND MYCOBACTERIAL ANTIGENE

Abstract. The article deals with results of studying combined effects of ethambutol and protein antigen of Мycobacterium tuberculosis upon proliferative response and cytokine-producing ability of peripheral blood mononuclear cells in patients with infiltrative lung tuberculosis prior to beginning of specific antibacterial therapy. The mononuclear blood cells were cultivated with complex protein antigen of Мycobacterium tuberculosis and ethambutol. In the patients with lung tuberculosis, a decrease in proliferative reserve of lymphocytes was detected, in absence of increased IL-2 and IL-10 secretion. The combined effect of ethambutol and protein antigen of Мycobacterium tuberculosis upon peripheral blood mononuclear cells in the patients with pulmonary tuberculosis and healthy donors was similar, and it was expressed as antiproliferative effect and selective suppression of IFNγ production

APOPTOSIS OF BLOOD LYMPHOCYTES IN THE PATIENTS WITH AUTOIMMUNE THYROID DISORDERS

Abstract. This article presents results of apoptosis detection among blood lymphocytes from the patients with autoimmune thyroid disorders, i.e., autoimmune thyroiditis (AIT) at euthyroid and hypothyroid stages, and in diffuse toxic goiter (DTG). Contents of CD95+, annexin V­ presenting (apoptotic) cells and TNFα in blood were analyzed. It was shown, that altered regulation of programmed lymphocyte death in autoimmune thyroid disorders is demonstrated by increased content of TNFα and CD95+ lymphocytes, being accompanied by decreased numbers of apoptotic cells in blood. Meanwhile, this pathology is expressed mostly in DTG, while being less significant in AIT at euthyroid stage, than in thyroid hypofunction. Possible mechanisms of the revealed disturbances are discussed. (Med. Immunol., 2008, vol. 10, N 2-3, pp 187-192)

REACTIVITY OF BLOOD LYMPHOCYTES IN PULMONARY TUBERCULOSIS

Evaluation of proliferative and IL-2-producing activity of peripheral blood lymphocytes wasperformed, using cultural methods, in patients with drug-sensitive and drug-resistant infiltrative pulmonary tuberculosis. The cell testing was performed at basal level and following in vitro stimulation with recombinant IL-2 and M. tuberculosis antigens. It was established that clinical course of infiltrative pulmonary tuberculosis, independently on drug sensitivity/resistance of the infectious pathogen, is accompanied by suppression of spontaneous lymphoproliferation. The levels of induced IL-2 production in drug-sensitive tuberculosis proved to be increased, whereas a reserve of IL-2-secreting reactivity of blood lymphocytes was lower than in drugresistant infection. Also, it was revealed that the level of lymphoproliferative response induced by IL-2, does not depend on clinical variant of tuberculosis, whereas stimulation of IL-2 production in blood lymphocytes is attained only in cases of drug-resistant tuberculosis variant