18 research outputs found

    Faraday instability on viscous ferrofluids in a horizontal magnetic field: Oblique rolls of arbitrary orientation

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    A linear stability analysis of the free surface of a horizontally unbounded ferrofluid layer of arbitrary depth subjected to vertical vibrations and a horizontal magnetic field is performed. A nonmonotonic dependence of the stability threshold on the magnetic field is found at high frequencies of the vibrations. The reasons of the decrease of the critical acceleration amplitude caused by a horizontal magnetic field are discussed. It is revealed that the magnetic field can be used to select the first unstable pattern of Faraday waves. In particular, a rhombic pattern as a superposition of two different oblique rolls can occur. A scaling law is presented which maps all data into one graph for the tested range of viscosities, frequencies, magnetic fields and layer thicknesses.Comment: 8 pages, 6 figures, RevTex

    Pattern formation in 2-frequency forced parametric waves

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    We present an experimental investigation of superlattice patterns generated on the surface of a fluid via parametric forcing with 2 commensurate frequencies. The spatio-temporal behavior of 4 qualitatively different types of superlattice patterns is described in detail. These states are generated via a number of different 3--wave resonant interactions. They occur either as symmetry--breaking bifurcations of hexagonal patterns composed of a single unstable mode or via nonlinear interactions between the two primary unstable modes generated by the two forcing frequencies. A coherent picture of these states together with the phase space in which they appear is presented. In addition, we describe a number of new superlattice states generated by 4--wave interactions that arise when symmetry constraints rule out 3--wave resonances.Comment: The paper contains 34 pages and 53 figures and provides an extensive review of both the theoretical and experimental work peformed in this syste

    End-tidal carbon dioxide monitoring during bag valve ventilation: the use of a new portable device

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    <p>Abstract</p> <p>Background</p> <p>For healthcare providers in the prehospital setting, bag-valve mask (BVM) ventilation could be as efficacious and safe as endotracheal intubation. To facilitate the evaluation of efficacious ventilation, capnographs have been further developed into small and convenient devices able to provide end- tidal carbon dioxide (ETCO<sub>2</sub>). The aim of this study was to investigate whether a new portable device (EMMAâ„¢) attached to a ventilation mask would provide ETCO<sub>2 </sub>values accurate enough to confirm proper BVM ventilation.</p> <p>Methods</p> <p>A prospective observational trial was conducted in a single level-2 centre. Twenty-two patients under general anaesthesia were manually ventilated. ETCO<sub>2 </sub>was measured every five minutes with the study device and venous PCO<sub>2 </sub>(PvCO<sub>2</sub>) was simultaneously measured for comparison. Bland- Altman plots were used to compare ETCO<sub>2, </sub>and PvCO<sub>2</sub>.</p> <p>Results</p> <p>The patients were all hemodynamically and respiratory stable during anaesthesia. End-tidal carbon dioxide values were corresponding to venous gases during BVM ventilation under optimal conditions. The bias, the mean of the differences between the two methods (device versus venous blood gases), for time points 1-4 ranges from -1.37 to -1.62.</p> <p>Conclusion</p> <p>The portable device, EMMAâ„¢ is suitable for determining carbon dioxide in expired air (kPa) as compared to simultaneous samples of PvCO<sub>2</sub>. It could therefore, be a supportive tool to asses the BVM ventilation in the demanding prehospital and emergency setting.</p

    Amplitude measurements of Faraday waves

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    A light reflection technique is used to measure quantitatively the surface elevation of Faraday waves. The performed measurements cover a wide parameter range of driving frequencies and sample viscosities. In the capillary wave regime the bifurcation diagrams exhibit a frequency independent scaling proportional to the wavelength. We also provide numerical simulations of the full Navier-Stokes equations, which are in quantitative agreement up to supercritical drive amplitudes of 20%. The validity of an existing perturbation analysis is found to be limited to 2.5% overcriticaly.Comment: 7 figure

    Derivation of Xeno-Free and GMP-Grade Human Embryonic Stem Cells – Platforms for Future Clinical Applications

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    Clinically compliant human embryonic stem cells (hESCs) should be developed in adherence to ethical standards, without risk of contamination by adventitious agents. Here we developed for the first time animal-component free and good manufacturing practice (GMP)-compliant hESCs. After vendor and raw material qualification, we derived xeno-free, GMP-grade feeders from umbilical cord tissue, and utilized them within a novel, xeno-free hESC culture system. We derived and characterized three hESC lines in adherence to regulations for embryo procurement, and good tissue, manufacturing and laboratory practices. To minimize freezing and thawing, we continuously expanded the lines from initial outgrowths and samples were cryopreserved as early stocks and banks. Batch release criteria included DNA-fingerprinting and HLA-typing for identity, characterization of pluripotency-associated marker expression, proliferation, karyotyping and differentiation in-vitro and in-vivo. These hESCs may be valuable for regenerative therapy. The ethical, scientific and regulatory methodology presented here may serve for development of additional clinical-grade hESCs
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