10 research outputs found
Praziquantel and Moxidectin pharmacokinetics in dogs after Helmimax administration
The purpose of the research is to study Praziquantel and Moxidectin pharmacokinetics in dogs after Helmimax administration.Materials and methods. Helmimax pharmacokinetics was studied on 8 adult male dogs of different breeds aged 2 to 5 years and weighing 15β35 kg. Helmimax was administered orally in the fasted state with a small amount of feed at a dose of 5 mg/kg for Praziquantel and 0.25 mg/kg for Moxidectin at the rate of 1 tablet per 10 kg of body weight. Blood was sampled at various periods after the administration. The collected blood underwent sample processing: formed element and protein precipitation, solid-phase extraction, and microfiltration. The active components were analyzed and detected by the HPLC-MS/MS. Active substances in the blood plasma were determined according to the developed technique which had been validated. The device was calibrated before the measurement.Results and discussion. As a result of the studies, the Praziquantel and Moxidectin pharmacokinetic parameters were calculated. The maximum concentration was 0.240 and 0.130 ΞΌg/mL, the time-to-peak concentration was 2.15 and 1.48 hours, and the elimination half-life was 8.41 and 3.61 hours for Moxidectin and Praziquantel, respectively
Π€Π°ΡΠΌΠ°ΠΊΠΎΠΊΠΈΠ½Π΅ΡΠΈΠΊΠ° ΠΏΡΠ°Π·ΠΈΠΊΠ²Π°Π½ΡΠ΅Π»Π° ΠΈ ΠΌΠΎΠΊΡΠΈΠ΄Π΅ΠΊΡΠΈΠ½Π° Π² ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ΅ ΡΠΎΠ±Π°ΠΊ ΠΏΠΎΡΠ»Π΅ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π³Π΅Π»ΡΠΌΠΈΠΌΠ°ΠΊΡΠ°
The purpose of the research is to study Praziquantel and Moxidectin pharmacokinetics in dogs after Helmimax administration.Materials and methods. Helmimax pharmacokinetics was studied on 8 adult male dogs of different breeds aged 2 to 5 years and weighing 15β35 kg. Helmimax was administered orally in the fasted state with a small amount of feed at a dose of 5 mg/kg for Praziquantel and 0.25 mg/kg for Moxidectin at the rate of 1 tablet per 10 kg of body weight. Blood was sampled at various periods after the administration. The collected blood underwent sample processing: formed element and protein precipitation, solid-phase extraction, and microfiltration. The active components were analyzed and detected by the HPLC-MS/MS. Active substances in the blood plasma were determined according to the developed technique which had been validated. The device was calibrated before the measurement.Results and discussion. As a result of the studies, the Praziquantel and Moxidectin pharmacokinetic parameters were calculated. The maximum concentration was 0.240 and 0.130 ΞΌg/mL, the time-to-peak concentration was 2.15 and 1.48 hours, and the elimination half-life was 8.41 and 3.61 hours for Moxidectin and Praziquantel, respectively.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ β ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΊΠΈΠ½Π΅ΡΠΈΠΊΠΈ ΠΏΡΠ°Π·ΠΈΠΊΠ²Π°Π½ΡΠ΅Π»Π° ΠΈ ΠΌΠΎΠΊΡΠΈΠ΄Π΅ΠΊΡΠΈΠ½Π° Π² ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ΅ ΡΠΎΠ±Π°ΠΊ ΠΏΠΎΡΠ»Π΅ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π³Π΅Π»ΡΠΌΠΈΠΌΠ°ΠΊΡΠ°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΊΠΈΠ½Π΅ΡΠΈΠΊΠΈ Π³Π΅Π»ΡΠΌΠΈΠΌΠ°ΠΊΡΠ° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° 8 Π²Π·ΡΠΎΡΠ»ΡΡ
ΡΠΎΠ±Π°ΠΊΠ°Ρ
ΡΠ°ΠΌΡΠ°Ρ
ΠΌΠ°ΡΡΠΎΠΉ ΡΠ΅Π»Π° 15β35 ΠΊΠ³ ΡΠ°Π·Π½ΡΡ
ΠΏΠΎΡΠΎΠ΄ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 2 Π΄ΠΎ 5 Π»Π΅Ρ. ΠΠ΅Π»ΡΠΌΠΈΠΌΠ°ΠΊΡ Π²Π²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΠ΅ΡΠΎΡΠ°Π»ΡΠ½ΠΎ Π½Π°ΡΠΎΡΠ°ΠΊ Ρ Π½Π΅Π±ΠΎΠ»ΡΡΠΈΠΌ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎΠΌ ΠΊΠΎΡΠΌΠ° Π² Π΄ΠΎΠ·Π΅ 5 ΠΌΠ³/ΠΊΠ³ ΠΏΠΎ ΠΏΡΠ°Π·ΠΈΠΊΠ²Π°Π½ΡΠ΅Π»Ρ ΠΈ 0,25 ΠΌΠ³/ΠΊΠ³ ΠΏΠΎ ΠΌΠΎΠΊΡΠΈΠ΄Π΅ΠΊΡΠΈΠ½Ρ ΠΈΠ· ΡΠ°ΡΡΠ΅ΡΠ° 1 ΡΠ°Π±Π»Π΅ΡΠΊΠ° Π½Π° 10 ΠΊΠ³ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π°. ΠΡΠ±ΠΎΡ ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΠ΅ΡΠ΅Π· ΡΠ°Π·Π»ΠΈΡΠ½ΡΠ΅ Π²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΠΈΠ½ΡΠ΅ΡΠ²Π°Π»Ρ ΠΏΠΎΡΠ»Π΅ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ. ΠΡΠΎΠ±ΡΠ°Π½Π½ΡΡ ΠΊΡΠΎΠ²Ρ ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π°Π»ΠΈ ΠΏΡΠΎΠ±ΠΎΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠ΅: ΠΎΡΠ°ΠΆΠ΄Π΅Π½ΠΈΠ΅ ΡΠΎΡΠΌΠ΅Π½Π½ΡΡ
ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ², Π±Π΅Π»ΠΊΠΎΠ², ΡΠ²Π΅ΡΠ΄ΠΎΡΠ°Π·Π½Π°Ρ ΡΠΊΡΡΡΠ°ΠΊΡΠΈΡ, ΠΌΠΈΠΊΡΠΎΡΠΈΠ»ΡΡΡΠ°ΡΠΈΡ. ΠΠ½Π°Π»ΠΈΠ· ΠΈ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π΄Π΅ΠΉΡΡΠ²ΡΡΡΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠΠΠ₯ ΠΠ‘/ΠΠ‘. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π΄Π΅ΠΉΡΡΠ²ΡΡΡΠΈΡ
Π²Π΅ΡΠ΅ΡΡΠ² Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΠΎ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΠΎΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ΅, ΠΊΠΎΡΠΎΡΠ°Ρ ΠΏΡΠΎΡΠ»Π° Π²Π°Π»ΠΈΠ΄Π°ΡΠΈΡ. ΠΠ΅ΡΠ΅Π΄ ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ ΠΊΠ°Π»ΠΈΠ±ΡΠΎΠ²ΠΊΡ ΠΏΡΠΈΠ±ΠΎΡΠ°.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈ ΠΎΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ Π±ΡΠ»ΠΈ ΡΠ°ΡΡΡΠΈΡΠ°Π½Ρ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΊΠΈΠ½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΏΡΠ°Π·ΠΈΠΊΠ²Π°Π½ΡΠ΅Π»Π° ΠΈ ΠΌΠΎΠΊΡΠΈΠ΄Π΅ΠΊΡΠΈΠ½Π°. ΠΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½Π°Ρ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ ΡΠΎΡΡΠ°Π²ΠΈΠ»Π° 0,240 ΠΈ 0,130 ΠΌΠΊΠ³/ΠΌΠ», Π²ΡΠ΅ΠΌΡ Π΄ΠΎΡΡΠΈΠΆΠ΅Π½ΠΈΡ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ 2,15 ΠΈ 1,48 Ρ, ΠΏΠ΅ΡΠΈΠΎΠ΄ ΠΏΠΎΠ»ΡΠ²ΡΠ²Π΅Π΄Π΅Π½ΠΈΡ 8,41 ΠΈ 3,61 Ρ Π΄Π»Ρ ΠΌΠΎΠΊΡΠΈΠ΄Π΅ΠΊΡΠΈΠ½Π° ΠΈ ΠΏΡΠ°Π·ΠΈΠΊΠ²Π°Π½ΡΠ΅Π»Π° ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ