Stressful conditions reveal decrease in size, modification of shape but relatively stable asymmetry in bumblebee wings

Human activities can generate a wide variety of direct and indirect effects on animals, which can manifest as environmental and genetic stressors. Several phenotypic markers have been proposed as indicators of these stressful conditions but have displayed contrasting results, depending, among others, on the phenotypic trait measured. Knowing the worldwide decline of multiple bumblebee species, it is important to understand these stressors and link them with the drivers of decline. We assessed the impact of several stressors (i.e. natural toxin-, parasite-, thermic- and inbreeding-stress) on both wing shape and size and their variability as well as their directional and fluctuating asymmetries. The total data set includes 650 individuals of Bombus terrestris (Hymenoptera: Apidae). Overall wing size and shape were affected by all the tested stressors. Except for the sinigrin (e.g. glucosinolate) stress, each stress implies a decrease of wing size. Size variance was affected by several stressors, contrary to shape variance that was affected by none of them. Although wing size directional and fluctuating asymmetries were significantly affected by sinigrin, parasites and high temperatures, neither directional nor fluctuating shape asymmetry was significantly affected by any tested stressor. Parasites and high temperatures led to the strongest phenotype modifications. Overall size and shape were the most sensitive morphological traits, which contrasts with the common view that fluctuating asymmetry is the major phenotypic marker of stress

TFEB regulates murine liver cell fate during development and regeneration

It is well established that pluripotent stem cells in fetal and postnatal liver (LPCs) can differentiate into both hepatocytes and cholangiocytes. However, the signaling pathways implicated in the differentiation of LPCs are still incompletely understood. Transcription Factor EB (TFEB), a master regulator of lysosomal biogenesis and autophagy, is known to be involved in osteoblast and myeloid differentiation, but its role in lineage commitment in the liver has not been investigated. Here we show that during development and upon regeneration TFEB drives the differentiation status of murine LPCs into the progenitor/cholangiocyte lineage while inhibiting hepatocyte differentiation. Genetic interaction studies show that Sox9, a marker of precursor and biliary cells, is a direct transcriptional target of TFEB and a primary mediator of its effects on liver cell fate. In summary, our findings identify an unexplored pathway that controls liver cell lineage commitment and whose dysregulation may play a role in biliary cancer

Genetic polymorphisms of the RAS-cytokine pathway and chronic kidney disease

Chronic kidney disease (CKD) in children is irreversible. It is associated with renal failure progression and atherosclerotic cardiovascular (CV) abnormalities. Nearly 60% of children with CKD are affected since birth with congenital or inherited kidney disorders. Preliminary evidence primarily from adult CKD studies indicates common genetic risk factors for CKD and atherosclerotic CV disease. Although multiple physiologic pathways share common genes for CKD and CV disease, substantial evidence supports our attention to the renin angiotensin system (RAS) and the interlinked inflammatory cascade because they modulate the progressions of renal and CV disease. Gene polymorphisms in the RAS-cytokine pathway, through altered gene expression of inflammatory cytokines, are potential factors that modulate the rate of CKD progression and CV abnormalities in patients with CKD. For studying such hypotheses, the cooperative efforts among scientific groups and the availability of robust and affordable technologies to genotype thousands of single nucleotide polymorphisms (SNPs) across the genome make genome-wide association studies an attractive paradigm for studying polygenic diseases such as CKD. Although attractive, such studies should be interpreted carefully, with a fundamental understanding of their potential weaknesses. Nevertheless, whole-genome association studies for diabetic nephropathy and future studies pertaining to other types of CKD will offer further insight for the development of targeted interventions to treat CKD and associated atherosclerotic CV abnormalities in the pediatric CKD population

The Southern Frontier of the Meroitic State: The View from Jebel Moya

The site of Jebel Moya, excavated in the early twentieth century, represents arguably the largest pastoral mortuary complex in Africa. Jebel Moya is resituated in relation to the neighbouring Meroitic-era agro-pastoral settlements and the only known Meroitic trading station (Sennar) in the southern Gezira Plain, Sudan. It is the first time that the known localities in the southern Gezira and southern Meroitic cemeteries have been compared, in an attempt to elucidate the different social organisation reflected in mortuary assemblages between the core and the periphery of the Meroitic State. New questions are posed for (1) the applicability of mortuary theory to pastoral cemeteries, and (2) the nature of zones of interaction on the frontier of the Meroitic State, through the application of new statistical and spatial analyses of the mortuary assemblages and the site’s reinterpretation as a pastoral, instead of an agro-pastoral, mortuary complex

Nucleotide-sequence of gene-2 of the UK tissue-culture adapted strain of Bovine Rotavirus

In this paper we demonstrate that our ability to match the EXtrinsic Information Transfer (EXIT) function of an Irregular Variable Length Code (IrVLC) to that of a serially-concatenated inner code depends on the availability of a suite of component Variable Length Error Correction (VLEC) codebooks having a wide variety of inverted EXIT function shapes. We also show that the inverted EXIT function shape of a VLEC codebook depends on its coding rate and Error Correction Capability (ECC). This motivates the design of a Genetic Algorithm (GA) that searches the large VLEC parameter space to find codebooks having specific coding rates, ECCs and, hence, EXIT function shapes. The employment of this GA therefore facilitates the design of component VLEC codebook suites without the manual trial-and-error that is required when employing the state-of-the-art Heuristic Algorithm (HA) used as our bench marker, which cannot design component VLEC codebooks having specific EXIT function shapes