Roles of the Drosophila SK Channel (dSK) in Courtship Memory

A role for SK channels in synaptic plasticity has been very well-characterized. However, in the absence of simple genetic animal models, their role in behavioral memory remains elusive. Here, we take advantage of Drosophila melanogaster with its single SK gene (dSK) and well-established courtship memory assay to investigate the contribution of this channel to memory. Using two independent dSK alleles, a null mutation and a dominant negative subunit, we show that while dSK negatively regulates the acquisition of short-term memory 30 min after a short training session, it is required for normal long-term memory 24 h after extended training. These findings highlight important functions for dSK in courtship memory and suggest that SK channels can mediate multiple forms of behavioral plasticity

Midline Signalling Systems Direct the Formation of a Neural Map by Dendritic Targeting in the Drosophila Motor System

During embryonic development of the motor system of Drosophila, motorneurons target their dendrites to different regions along the body axis in response to midline guidance cues

Articulação prático-teórica e a produção de inovação no mestrado profissional em Ensino em Ciências da Saúde

Este artigo tem como objetivo analisar a experiência da disciplina de pós-graduação Grupo de Estudos e Pesquisas sobre as Práticas (GEPPRA) do Programa de Mestrado Profissional em Ensino em Ciências da Saúde, da Universidade Federal de São Paulo UNIFESP, campus Baixada Santista, com foco na metodologia dos escribas, sua potência como método de ensino-aprendizagem e como fonte de reflexão. O corpus analisado foi de 14 registros elaborados por estudantes e docentes, e as etapas de análise foram: análise pragmática automática dos textos com auxílio do programa Alceste; análise de conteúdo dos trechos que continham as palavras-chave: Geppra (Geppra I, Geppra II), Escriba (escrito, escrever) e Trabalho (fazer, prática e reflexão) para identificar alguns sentidos atribuídos ao GEPPRA; a metodologia dos escribas; e efeitos da disciplina na prática profissional. Os resultados apontam que foi possível construir coletivamente uma nova textualidade em rede, a partir de uma experiência reflexiva, com troca, discussão e questionamento sobre as maneiras de lidar com situações desafiadoras no trabalho. Afirma-se a potência da ferramenta dos escribas e o seu uso em outros contextos de formação ou nos processos grupais, sejam acadêmicos e/ou clínicos

The neuroimmune guidance cue netrin-1 promotes atherosclerosis by inhibiting the emigration of macrophages from plaques

Nephrolog

National Trends for Reverse Shoulder Arthroplasty After the Affordable Care Act: An Analysis From 2011 to 2015.

Few studies have investigated nationwide patient trends and health care costs for reverse shoulder arthroplasty (RSA) after 2014. This study uses a large validated nationwide database to retrospectively assess changes in patient and hospital demographic features, hospital costs, and hospital charges for inpatient RSA procedures before and after implementation of the Affordable Care Act. The National Inpatient Sample database was used to identify all patients who underwent RSA between January 2011 and December 2015, yielding 163,171 patients (63.4% female; mean age, 72 years). Categorical data were assessed with chi-square/Fisher\u27s exact test, and continuous data were assessed with analysis of variance. There was an increased proportion of RSA recipients identifying as Hispanic (4.1% to 4.8%) and Native American (0.1% to 0.4%; P\u3c.0001). The proportion of patients who had Medicaid (1.4% to 2.4%) and private insurance (15.1% to 16.6%) increased as well (P\u3c.0001). A decrease in mean hospital costs occurred between 2011 and 2015 (-$256; P=.002), whereas an increase occurred in hospital charges (+$6,314; P\u3c.001). These findings provide insight on RSA use and patient demographic trends in the United States. Additionally, these results help to capture the effects of extended health coverage and new reimbursement models on hospital costs and charges

NDST1 missense mutations in autosomal recessive intellectual disability

NDST1 was recently proposed as a candidate gene for autosomal recessive intellectual disability in two families. It encodes a bifunctional GlcNAc N-deacetylase/N-sulfotransferase with important functions in heparan sulfate biosynthesis. In mice, Ndst1 is crucial for embryonic development and homozygous null mutations are perinatally lethal. We now report on two additional unrelated families with homozygous missense NDST1 mutations. All mutations described to date predict the substitution of conserved amino acids in the sulfotransferase domain, and mutation modeling predicts drastic alterations in the local protein conformation. Comparing the four families, we noticed significant overlap in the clinical features, including both demonstrated and apparent intellectual disability, muscular hypotonia, epilepsy, and postnatal growth deficiency. Furthermore, in Drosophila, knockdown of sulfateless, the NDST ortholog, impairs long-term memory, highlighting its function in cognition. Our data confirm NDST1 mutations as a cause of autosomal recessive intellectual disability with a distinctive phenotype, and support an important function of NDST1 in human development