Oxidative stress parameters changes in blood of rats treated with adriamycin and selenium

Meeting on Free Radicals and Oxidative Stress, 2003, Loannina, Greec

Effect of gamma irradiation on magnesium content in rat tissues

Studies in the field of radiation chemistry have shown that the greatest damage induced by ionizing radiation appears as a consequence of uncontrolled production of free radicals in the living organism, which have their origin in oxidative stress induced by ionizing radiation. Ionizing radiation also has an influence on the content of bio-essential elements in mammalian tissues. Analysis of the concentration of magnesium (Mg) seven days after whole body irradiation with a single dose of 4.2 Gy of gamma rays from a Co-60 source, showed a slight reduction in Mg content in the following analyzed tissues: front brain (cerebral hemispheres with underlying structures except pituitary gland and hypothalamus) and adrenal glands from irradiated animals as compared to the non-irradiated controls, this difference however being not statistically significant. No significant differences in the content of Mg were detected in the pituitary glands between the two groups of animals. In contrast, a significantly higher concentration of Mg seven days after irradiation was measured in the hind brain-containing cerebellum with lateral lobes and flocculi (p LT 0.005), hypothalamus (p LT 0.0001) and testes (p LT 0.05) of irradiated animals, when compared to the non-irradiated controls. This accumulation of Mg, in particular tissues of the Central Nervous System (CNS) and endocrine glands regulated by the pituitary gland and hypothalamus, may indicate the differential role Mg++ ions have in keeping the homeostasis of certain tissues, those of the brain, adrenal glands and testes after exposure to ionizing radiation

Influence of long-term selenium supplementation on antioxidant trace elements in the rat brain

Metal Ions in Biology and Medicine, 8th International Symposium on Metal Ions in Biology and Medicine, May 18-22, 2004, Hungarian Acad Sci, Budapest, Hungar

Influence of long-term selenium supplementation on antioxidant trace elements in the rat brain

Metal Ions in Biology and Medicine, 8th International Symposium on Metal Ions in Biology and Medicine, May 18-22, 2004, Hungarian Acad Sci, Budapest, Hungar

Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy

Tiazofurin (TZF) is a cytostatic drug that leads to depletion of the GTP pool in tumor and normal cells via its active metabolite tiazofurin-adenine dinucleotide (TAD). TAD was detected in different cell lines, but not in erythrocytes, so the mechanism of erythrocytotoxicity of TZF remains unclear. The purpose of this study was to evaluate in vitro and in vivo action of tiazofurin on rat erythrocytes (RBC). After two decades of clinical trials the question of erythrocytotoxicity of TZF had remained unexplained making this study justified. Since we have previously demonstrated early erythrocytotoxic effects in male Wistar rats, we extend this finding on isolated RBC. Isolated erythrocytes from untreated animals were treated in buffered solution or plasma containing TZF In addition, groups of 10 rats were treated with 200 and 1000 mg/kg of TZF and hematologic parameters were analyzed by flowcytometry and by the analysis of the peripheral blood smears. Early signs of hemolysis or aberrant structures were monitored by scanning probe microscopy (SPM). We suggest that correlation exists between early erythrocytotoxicity and irregularities in erythrocyte morphology and membrane integrity. We also found that TZF affects responsiveness to oxidative stress. This is in concordance with flowcytometric findings describing anisocytosis and anisochromosis of RBC. (C) 2003 Elsevier Ireland Ltd. All rights reserved