10 research outputs found
Patients with hepatic breast cancer metastases demonstrate highly specific profiles of matrix metalloproteinases MMP-2 and MMP-9 after SIRT treatment as compared to other primary and secondary liver tumours
Full text linkInnovative strategies for prediction and targeted prevention of glaucoma in healthy vasospastic individuals: context of neurodegenerative pathologies
No full textOptimal multiparametric set-up modelled for best survival outcomes in palliative treatment of liver malignancies: unsupervised machine learning and 3 PM recommendations
No full textPredictive molecular profiling in blood of healthy vasospastic individuals: clue to targeted prevention as personalised medicine to effective costs
No full textMulti-omic approach decodes paradoxes of the triple-negative breast cancer: lessons for predictive, preventive and personalised medicine
No full textKey molecular pathways affected by glaucoma pathology: is predictive diagnosis possible?
No full textPremenopausal breast cancer: potential clinical utility of a multi-omics based machine learning approach for patient stratification
No full textMatrix metalloproteinase 9 expression: new regulatory elements
No full textRetinal ganglion cells apoptosis is linked to matrix metalloproteinase 9 (MMP-9) controlled changes of extracellular matrix. Abnormal expression of MMP-9 is associated with glaucomatous alterations. Thus, the knowledge of MMP-9 regulation is important for the understanding the pathogenesis of glaucoma. Here, we investigated the role of 3′-untranslated regions (3′-UTR) and microRNAs in MMP-9 regulation. We used in vitro mutagenesis and Luc reporter system to identify regulatory elements in the 3′-UTR of MMP-9. microRNAs were analyzed by qRT-PCR, and their role was investigated with inhibitors and mimics. We identified targets for miRNAs in 3′-UTR of MMP-9 involved in the regulation of MMP-9 expression. We then isolated miRNAs from the optic nerve A7 astrocytes and 293 T cells and confirmed the role of mi340 in the regulation using specific inhibitors and mimics. The results obtained show a new miRNA-mediated mechanism of MMP-9 expression regulation
