T cell lymphoma in the atrium of a young bull

In this report, T-cell lymphoma in the atrium of a 1.5-year-old Holstein bull is described. Macro-scopically, a large white to a yellow tumour mass with a size of 4.5×3.5×2 cm was observed in the left atrium. Histopathological examination revealed extensive infiltration of medium- to large-sized lymphocytic cells with round to oval nuclei and stippled chromatin, surrounded by a narrow rim of pale eosinophilic cytoplasm. Immunohistochemically, the tumour cells indicated positivity with CD3 and Ki-67, but negativity with CD79α, CD20 and S100. On the basis of the histologic and immuno-histochemical findings, this tumour was diagnosed as a T-cell lymphoma

Coccidiosis due to various species of Eimeria in the stunted and diarrheic native turkey poults: Pathology and morphological characterization of oocysts

Fecal samples of 60 turkey poults that showed chronic progressive symptoms like unthriftiness, loss ofweight, diarrhea were collected from the most rural areas with high rate of turkey population in north andwest part of country for intestinal protozoan parasites. According to the morphological characteristics, likeshape, presence or absence of micropyle, and/or polar granule, the 5 different types of eimerian oocycts were diagnosed in the stool of infected birds, including E. adenoids, E. meleagridis, E. dispersa, Eimeria spp (E. innocua or E. subrotunda) and E. meleagrimitis. Various life- cycle stages of Eimeria were identified in the epithelial lining of inflamed intestine of the affected turkey poults

Streptozotocin-induced Diabetic Effects on the Sperm Fertility Parameters, Glycated Hemoglobin and Total Cholesterol in Mice

Introduction: The present study aimed to evaluate the effects of type 1 diabetes model induced by streptozotocin (STZ) on the sperm fertility parameters, glycated hemoglobin (HbA1c) and total cholesterol as lipid metabolism indicators. Methods: Induction of diabetes mellitus was achieved through once subcutaneous (SC) injection of 200 mg/kg body weight of STZ in 10 mice, while the control group recived 0.25 ml of disstiled water in the same method. For diabetic groups, 8 mice were selected among the animals with higher glucose threshold (350 mg/dl) one week after STZ injection. Two weeks after diabetes confirmation, all the mice were sacrificed and whole blood was taken from cardiac puncture, while testes were removed for histopathological study and sperm evaluation. Total sperm motility, progresive movement, hypoosmotic swelling test and sperm concentration were determined by the conventional methods. Moreover, HbA1c and total cholesterol were measured spectrophotometerically. Results: Diabetic animals showed a significant decrease in sperm count (P <0.05), as well as an increase in HbA1c and total cholesterol (P <0.01) compared to the controls. A significant negative linear reletionship (R2=0.90, P=0.012) was depicted between HbA1c and sperm concentration in the diabetic group. Epithelial vaculization of semineferous tubules was observed in the diabetic group in comparison with the control group and epidydimal ducts indicated increased volume of spermatoza in the controls when compared to the diabetic rats. Conclusion: As the study findings revealed diabetes was associated with decreased sperm concentration in the cauda epididymis even two weeks after hyperglycemia induction, which can be mentioned as a predective index for subfertility. Furthermore, Glycated hemoglobin revealed a negetive relationship with sperm concentration. Hypercholestrolemia was also shown in early stages of diabetes indicating an impairment in lipids metabolism, which was confirmed by the histology changes

The effects of Post-Hatch Feeding with Betaine on the Intestinal Development of Broiler Chickens

ABSTRACT The capacity of the chicken intestinal tract to digest and absorb nutrients is not fully developed on the day of hatch. The aim of this study was to investigate the role of betaine on the intestinal development of broiler chickens, including brush border enzyme activities indices and morphological changes during the first week of growth. Fiftyone-day-old Ross 308 broiler chickens were randomly distributed into five groups: control (C), low methionine(LM), low methionine plus betaine (LM+B), betaine 1 (B1) and betaine 2 (B2). Betaine was dissolved with a concentration of 500 mg/L in the drinking water of LM+B and B1groups and a concentration of 1000 mg/L in the B2 group. Histological assessment showed lower duodenal crypt depth and villi height/crypt depth ratio in B1 as compared to C. Jejunal villi height of B2birds was significantly higher than that of C birds, whereas jejunal crypt depth values of both B2 and LM birds were higher relative to LM+B and C birds. Betaine-fed groups had significantly a thicker ileal muscularis mucosa layer than C and LM groups. Duodenal and jejunal g-glutamyl transferase activity was significantly higher in B2birds in LM birds. Alkaline phosphatase activity in all three segments of the small intestine was significantly higher in the LM birds than in other groups. Jejunal alanine aminopeptidase activity in LM birds was significantly higher than in other groups, particularly in B1 and B2. The results suggest that betaine may be a promising agent to promote the intestinal development of broilers fed low-methionine or standard diets

Peptite-coupling reagent causing cutaneous and respiratory reactions: multidisciplinary investigation

Synthesis of peptides requires addition of amino-acid residue through liquid and solid phase process using coupling reagents such as salts of hydroxybenzotriazole (HOBt). The uronium compound TBTU (2-(1H-benzotriazol-1-y1)-1,1,3,3-tetramethyluronium hexafluorophophate) provide efficient incorporation of amino acids minimizing racemization