9 research outputs found
Human Placental Lipid Induces Mitogenesis and Melanogenesis in B16F10 Melanoma Cells
A hydroalcoholic extract of fresh term human placenta was found to be mitogenic as well as melanogenic on
B16F10 mouse melanoma in an in vitro culture. The extract, a reservoir of a large number of bioactive
molecules, was resolved to get the lipid fraction. Its activity was evaluated on B16F10 mouse melanoma by
assessing the change in cellular morphology, growth and melanin induction. The lipid fraction, placental total
lipid fraction (PTLF) tested in the study employed doses of 0×01 to 200 mg/ml; optimum growth and melanization
accompanied by morphological changes were recorded at 10 and 100 mg/ml respectively. At intermediate
doses growth and melanization were found to show a pattern of change over between growth and melanization
and finally reached at an inverse relation at the respective optimal dose of response. Compared with defined
sphingolipids, C2 ceramide and sphingosine-1-phosphate, the results were mostly corroborative. The duality of
biological response of sphingolipids as reported in numerous studies was comparable for the PTLF suggesting
that its active component is a sphingolipid and showing its use for pigment recovery in vitiligo