"For good design, you pay now; for bad design, you pay later"--or do you?

Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Urban Studies and Planning, 2006.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Includes bibliographical references (leaves 117-118).What is the value of architectural design on office building income? This empirical study of 296 office building located in 11 Metropolitan Statistical Areas (MSA) hopes to quantitatively determine if a plain vanilla cereal box suburban office building commands more or less net operating income than an office building with a higher level of design. Previous empirical studies have found a strong influence of design on rents but were limited in geography, building characteristics and total number of observations. In an important study by Vandell and Lane (1990), they found that good architecture commanded a premium of over 20% in office rents. Also, their study showed that good design cost more to produce on average, but not necessarily in every case. Data was gathered from a portfolio of US office buildings and consisted of building metrics and property level 2000-2005 Net Operating Income (NOI). This base data set, MSA dummy variables and architectural attribute dummy variables (created by the authors) formed the backbone of the research. Multiple log linear regression analysis was conducted to identify the economic effects of good design.(cont.) In addition, a survey taken by 31 architects was used to capture subjective rankings on the all 296 office buildings to determine if there is a consensus as to what constitutes good design. It is hoped that these professionals, who are formally trained and are practicing in the field, are well-qualified to evaluate the design of each building. The survey results showed that the architects' responses are idiosyncratic and subjective. Not only did the individual participant's rankings show no significant relationship with one another, but also did not exhibit any relationship with actual building NOI. The empirical study found that the market paid a premium of 7.9% for buildings with non-center cores. Also, a significant 11.7-13.2% premium was paid for properties with non-rectangular and non-square shaped floor plans. Finally, buildings with 60% to 90% exterior windows commanded a substantial 10.7% premium. These results imply that better-designed buildings generate higher NOI either because the tenants are willing to pay a premium or because the operating costs of the building are less, or both.by Meena Murugappan and S. Michael O'Young, Jr.S.M

JSBSim Open-Source Flight Dynamics Model for Fixed-Wing Unmanned Aerial Vehicle Applications

Simulation packages provide a valuable framework or environment to study the interaction between aircraft, including Unmanned Aerial Vehicles (UAVs), in the existent air traffic in near mid-air collision (NMAC) scenarios. The proposed simulation package is based on the open-source JSBSim Flight Dynamics Model (FDM) and our developments represent the UAVs with the required precision to model those interactions. This tool has been validated and tested in UAV computer models and is currently being used in 4D encounters and avoidance maneuvers. The final objective of this paper is to provide a simplified model version of the current package, the including the minimum requirements for the design of a UAV in JSBSim, and guide any modeler through the UAV computer design task

Comparison of Medium and Long Wave Infrared Imaging for Ocean Based Sensing

Infrared thermal imagers convert the radiation received by a detector into a false colour visual image which represents the temperature profile of a scene or object. The selection of an infrared sensor for ocean based imaging is complicated, and depends on the intended use and target characteristics. This paper provides a theoretical understanding of the physics associated with the energy collected by the sensor and the resulting infrared images. Further, explanation of the factors affecting the resulting image with respect to the camera properties is provided. Finally, a variety of examples of airborne thermal images are presented, with detailed explanations of the imaged scenes based on theory and sensor characteristics provided in the previous sections

Quality of life in Type 1 (insulin-dependent) diabetic patients prior to and after pancreas and kidney transplantation in relation to organ function

Improvement of the quality of life in Type 1 (insulin-dependent) diabetic patients with severe late complications is one of the main goals of pancreas and/or kidney grafting. To assess the influences of these treatment modalities on the different aspects of the quality of life a cross-sectional study in 157 patients was conducted. They were categorized into patients pre-transplant without dialysis (n=29; Group A), pre-transplant under dialysis (n=44; Group B), post-transplant with pancreas and kidney functioning (n=31; Group C), post-transplant with functioning kidney, but insulin therapy (n=29; Group D), post-transplant under dialysis and insulin therapy again (n=15; Group E) and patients after single pancreas transplantation and rejection, with good renal function, but insulin therapy (n=9; Group F). All patients answered a mailed, self-administered questionnaire (217 questions) consisting of a broad spectrum of rehabilitation criteria. The results indicate a better quality of life in Groups C and D as compared to the other groups. In general the scores are highest in C, but without any significant difference to D. Impressive significant differences between C or D and the other groups were found especially in their satisfaction with physical capacity, leisure-time activities or the overall quality of life. The satisfaction with the latter is highest in C (mean±SEM: 4.0±0.2 on a 1 to 5-rating scale; significantly different from A: 3.1±0.1, B: 2.7±0.2 and E: 2.6±0.3; p<0.01), followed by D (3.8±0.2; significantly different from B and E; p<0.01). Group F shows a mean of 3.1±0.4, which is not significantly different from C. The percentages of patients in each group, who are not working: A: 38 %, B: 64 %, C: 74 %, D: 66 %, E: 87 % and F: 78 % indicate that there is no marked improvement in the vocational situation after successful grafting

CTCF genetic alterations in endometrial carcinoma are pro-tumorigenic

CTCF is a haploinsufficient tumour suppressor gene with diverse normal functions in genome structure and gene regulation. However the mechanism by which CTCF haploinsufficiency contributes to cancer development is not well understood. CTCF is frequently mutated in endometrial cancer. Here we show that most CTCF mutations effectively result in CTCF haploinsufficiency through nonsense-mediated decay of mutant transcripts, or loss-of-function missense mutation. Conversely, we identified a recurrent CTCF mutation K365T, which alters a DNA binding residue, and acts as a gain-of-function mutation enhancing cell survival. CTCF genetic deletion occurs predominantly in poor prognosis serous subtype tumours, and this genetic deletion is associated with poor overall survival. In addition, we have shown that CTCF haploinsufficiency also occurs in poor prognosis endometrial clear cell carcinomas and has some association with endometrial cancer relapse and metastasis. Using shRNA targeting CTCF to recapitulate CTCF haploinsufficiency, we have identified a novel role for CTCF in the regulation of cellular polarity of endometrial glandular epithelium. Overall, we have identified two novel pro-tumorigenic roles (promoting cell survival and altering cell polarity) for genetic alterations of CTCF in endometrial cance

Structure–function relationships explain CTCF zinc finger mutation phenotypes in cancer

CCCTC-binding factor (CTCF) plays fundamental roles in transcriptional regulation and chromatin architecture maintenance. CTCF is also a tumour suppressor frequently mutated in cancer, however, the structural and functional impact of mutations have not been examined. We performed molecular and structural characterisation of five cancer-specific CTCF missense zinc finger (ZF) mutations occurring within key intra- and inter-ZF residues. Functional characterisation of CTCF ZF mutations revealed a complete (L309P, R339W, R377H) or intermediate (R339Q) abrogation as well as an enhancement (G420D) of the anti-proliferative effects of CTCF. DNA binding at select sites was disrupted and transcriptional regulatory activities abrogated. Molecular docking and molecular dynamics confirmed that mutations in residues specifically contacting DNA bases or backbone exhibited loss of DNA binding. However, R339Q and G420D were stabilised by the formation of new primary DNA bonds, contributing to gain-of-function. Our data confirm that a spectrum of loss-, change- and gain-of-function impacts on CTCF zinc fingers are observed in cell growth regulation and gene regulatory activities. Hence, diverse cellular phenotypes of mutant CTCF are clearly explained by examining structure–function relationships

Roles of Electrostatics and Conformation in Protein-Crystal Interactions

In vitro studies have shown that the phosphoprotein osteopontin (OPN) inhibits the nucleation and growth of hydroxyapatite (HA) and other biominerals. In vivo, OPN is believed to prevent the calcification of soft tissues. However, the nature of the interaction between OPN and HA is not understood. In the computational part of the present study, we used molecular dynamics simulations to predict the adsorption of 19 peptides, each 16 amino acids long and collectively covering the entire sequence of OPN, to the {100} face of HA. This analysis showed that there is an inverse relationship between predicted strength of adsorption and peptide isoelectric point (P<0.0001). Analysis of the OPN sequence by PONDR (Predictor of Naturally Disordered Regions) indicated that OPN sequences predicted to adsorb well to HA are highly disordered. In the experimental part of the study, we synthesized phosphorylated and non-phosphorylated peptides corresponding to OPN sequences 65–80 (pSHDHMDDDDDDDDDGD) and 220–235 (pSHEpSTEQSDAIDpSAEK). In agreement with the PONDR analysis, these were shown by circular dichroism spectroscopy to be largely disordered. A constant-composition/seeded growth assay was used to assess the HA-inhibiting potencies of the synthetic peptides. The phosphorylated versions of OPN65-80 (IC50 = 1.93 µg/ml) and OPN220-235 (IC50 = 1.48 µg/ml) are potent inhibitors of HA growth, as is the nonphosphorylated version of OPN65-80 (IC50 = 2.97 µg/ml); the nonphosphorylated version of OPN220-235 has no measurable inhibitory activity. These findings suggest that the adsorption of acidic proteins to Ca2+-rich crystal faces of biominerals is governed by electrostatics and is facilitated by conformational flexibility of the polypeptide chain