Investigation of dose-dependent effect of curcumin on T-47D breast cancer cells

Zingiberaceae familyasına ait zerdeçaldan elde edilen bir polifenol olan kurkumin, anti-inflamatuar, anti-tümör, anti-oksidatif ve antimikrobiyal etkiler dahil olmak üzere birçok etkiye sahiptir. Kurkuminin farklı kanser hücreleri üzerindeki etkileri hakkında birçok çalışma bulunmaktadır. Bu çalışma, kurkuminin T-47D meme kanseri hücre canlılığı üzerindeki anti-kanser etkisini araştırmayı amaçlamaktadır. T-47D meme kanseri hücrelerine farklı dozlarda uygulanan kurkuminin etkisi MTT yöntemi ve inverted mikroskop ile araştırılmıştır. Kurkuminin T-47D hücrelerinde IC50 dozu 24 saat sonunda 65,8 µM, 48 saat sonunda 46,4 µM ve 72 saat sonunda ise 26,6 µM olarak belirlenmiştir. Morfolojik değerlendirmede ise kurkumin uygulanmış hücreler yuvarlak ve flask yüzeyinden ayrılmış kitleler halinde gözlenmektedir. Sonuçlarımız, kurkuminin T-47D hücre proliferasyonunu önemli ölçüde azalttığını göstermektedir. Kurkumin, tek başına veya diğer moleküllerle kombinasyon halinde meme kanseri tedavisi için bir aday olabilir. Gelecekte, kurkuminin meme kanseri hücreleri üzerindeki etki mekanizmasını aydınlatmak için daha kapsamlı ve çok merkezli destekli ileri klinik çalışmalara ihtiyaç vardır.Curcumin, a polyphenol derived from turmeric belonging to the Zingiberaceae family, has multiple effects, including anti-inflammatory, antitumor, anti-oxidative and antimicrobial effects. There are many studies on the effects of curcumin on different cancer cells. This study aims at investigating the anti-cancer effect of curcumin on T-47D breast cancer cell. T-47D breast cancer cells will be examined by MTT assay and inverted microscopy. IC50 doses are 65.8 µM for 24 h-long MTT, 46.4µM for 48 h-long MTT and 26.6 µM for 72 h-long MTT assay. In the inverted microscope, curcumin-treated cells appear to be rounded and detached from the bottom flask with a massive morphology. Our results showed that curcumin significantly reduced T-47D cell proliferation. Curcumin may be a candidate for breast cancer therapy, either alone or in combination with other molecules. In the future, more comprehensive and multicenter-supported advanced clinical studies are needed to elucidate the mechanism of action of curcumin on breast cancer cells

Comparison of the Effects and Mechanism of the Curcumin with Different Drugs in Experimental Vasospasm After Subarachnoid Hemorrhage

AIM: Cerebral vasospasm following subarachnoid hemorrhage (SAH) is the most important complication that effects the mortality and morbidity of patients with intracranial aneurysm. Today, the mechanisms of vasospasm are not understood in spite of experimental and clinical researches. The aim of our study was to investigate the effects of curcumin on vasospasm following SAH

Cardiac Hypertrophy Caused by Hyperthyroidism in Rats: Role of ATF-6 and TRPC1 Channels

Hyperthyroidism influences the development of cardiac hypertrophy. Transient receptor potential canonical channels (TRPCs) and ER stress are regarded as critical pathways in cardiac hypertrophy.Hence, we aimed to identify the TRPCs associated with ER stress in hyperthyroidism-induced cardiac hypertrophy.20 adult Wistar albino male rats were used in the study.The control group was fed with standard food and tap water. The group with hyperthyroidism was also fed with standard rat food, along with tap water that contained 12 mg/L of thyroxine for four weeks.At the end of the fourth week, the serum-free T3, T4, and TSH levels of the groups were measured. The left ventricle of each rat was used for histochemistry, immunohistochemistry, western blot, total antioxidant capacity (TAC), and total oxidant status (TOS) analysis. As per our results, ATF-6, IRE-1, and TRPC1, which play a significant role in cardiac hypertrophy caused by hyperthyroidism, showed increased activation. Moreover, TOS and fT3 levels increased, while TAC and TSH levels decreased. With the help of the literature review in our study, we could, for the first time, indicate that the increased activation, in particular of ATF-6, IRE-1, and TRPC1-induced deterioration of the Ca2+ ion balance, leads to hypertrophy in hyperthyroidism due to heart failure.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Neuroprotective Effect of Genistein in Peripheral Nerve Injury

AIM: To investigate the effects of genistein in a rat model of sciatic nerve crush injury and complete sciatic nerve transection. The effects of genistein were compared with those of gabapentin, which is widely used in clinical practice for peripheral nerve injury