‘Nice to Meet You Again’: When Heutagogy Met Blended Learning in Teacher Education, Post-Pandemic Era

Heutagogy and blended learning (BL) are core concepts in the educational discourse post-COVID-19. Conducting a mixed-methods study, we investigate meeting points between heutagogy principles and BL in the context of curricular change in the academic timetable of teacher education college, where pre-COVID most courses have been taught face-to-face (F2F). At present, teacher educators and students meet F2F for three weeks, followed by a week of remote learning, combining synchronous and asynchronous pedagogies. Data have been collected by a closed-ended questionnaire and two focus groups, involving altogether 76 lecturers and 553 students. Findings indicate that heutagogy has been applied in all facets of BL, rather than only with online or digital technological components. This study explores a bottom-up growth of heutagogy expressions in BL at three meeting points. When the core facets of heutagogy principles have been identified, there has been a predominance of the students’ agency and life-long learners, together with facets such as a non-linear learning and capability development that have been underrepresented. This study contributes to the research field of heutagogy in teacher education as it identifies the meaning and the way a structural change in the curriculum can constitute an accelerator and catalyst when implementing heutagogy in practice

Molecular Mechanisms in Murine Syngeneic Leukemia Stem Cells

Acute Myeloid Leukemia (AML) is a severe disease with a very high relapse rate. AML relapse may be attributable to leukemic stem cells (LSC). Notably, the “cancer stem cell” theory, which relates to LSCs, is controversial and criticized due to the technical peculiarities of the xenotransplant of human cells into mice. In this study, we searched for possible LSCs in an immunocompetent synergetic mice model. First, we found phenotypic heterogeneity in the ML23 leukemia line. We prospectively isolated a sub-population using the surface markers cKit+CD9−CD48+Mac1−/low, which have the potency to relapse the disease. Importantly, this sub-population can pass in syngeneic hosts and retrieve the heterogeneity of the parental ML23 leukemia line. The LSC sub-population resides in various organs. We present a unique gene expression signature of the LSC in the ML23 model compared to the other sub-populations. Interestingly, the ML23 LSC sub-population expresses therapeutic targeted genes such as CD47 and CD93. Taken together, we present the identification and molecular characterization of LSCs in a syngeneic murine model

Molecular Mechanisms in Murine Syngeneic Leukemia Stem Cells

Acute Myeloid Leukemia (AML) is a severe disease with a very high relapse rate. AML relapse may be attributable to leukemic stem cells (LSC). Notably, the “cancer stem cell” theory, which relates to LSCs, is controversial and criticized due to the technical peculiarities of the xenotransplant of human cells into mice. In this study, we searched for possible LSCs in an immunocompetent synergetic mice model. First, we found phenotypic heterogeneity in the ML23 leukemia line. We prospectively isolated a sub-population using the surface markers cKit+CD9−CD48+Mac1−/low, which have the potency to relapse the disease. Importantly, this sub-population can pass in syngeneic hosts and retrieve the heterogeneity of the parental ML23 leukemia line. The LSC sub-population resides in various organs. We present a unique gene expression signature of the LSC in the ML23 model compared to the other sub-populations. Interestingly, the ML23 LSC sub-population expresses therapeutic targeted genes such as CD47 and CD93. Taken together, we present the identification and molecular characterization of LSCs in a syngeneic murine model