Neuroprotection of Tropical Fruit Juice Mixture via the Reduction of iNOS Expression and CRH Level in β-Amyloid-Induced Rats Model of Alzheimer’s Disease

This study aimed to determine the effects of tropical fruit juice mixture (pomegranate, white guava, and Roselle) on biochemical, behavioral, and histopathological changes of β-amyloid- (Aβ-) induced rats. Formulation 8 (F8) of tropical fruit juice mixture was chosen for this present study due to its high phenolic content and antioxidant capacity. Forty Wistar male rats were divided into five groups: dPBS (sham-operated control), dAβ (Aβ control), JPBS (F8 and PBS), JAβ (F8 and Aβ), and IBFAβ (ibuprofen and Aβ). F8 (5 ml/kg BW), and ibuprofen (10 ml/kg BW) was given orally daily for four weeks before the intracerebroventricular infusion of Aβ for two weeks. Histological analysis and neuronal count of hippocampus tissue in the Cornu Ammonis (CA1) region showed that supplementation with F8 was able to prevent Aβ-induced tissue damage and neuronal shrinkage. However, no significant difference in locomotor activity and novel object recognition (NOR) percentage was detected among different groups at day 7 and day 14 following Aβ infusion. Only effect of time differences (main effect of day) was observed at day 7 as compared to day 14, where reduction in locomotor activity and NOR percentage was observed in all groups, with F (1, 7) = 6.940, p<0.05 and F (1, 7) = 7.152, p<0.05, respectively. Besides, the MDA level of the JAβ group was significantly lower (p<0.01) than that of the dPBS group. However, no significant changes in SOD activity were detected among different groups. Significant reduction in plasma CRH level (p<0.05) and iNOS expression (p<0.01) in the brain was detected in the JAβ group as compared to the dAβ group. Hence, our current findings suggest that the tropical fruit juice mixture (F8) has the potential to protect the rats from Aβ-induced neurotoxicity in brain hippocampus tissue possibly via its antioxidant properties and the suppression of iNOS expression and CRH production

Spatial Distribution of COVID-19 Infected Cases in Kelantan, Malaysia

Kota Bharu city in Kelantan, Malaysia was reported with the highest cases of coronavirus disease 2019 (COVID-19) among other districts. Kota Bharu is the capital city of Kelantan, which acts as the administrative, commercial, and financial areas. A large population pool may become a potential carrier for disease transmission to become an epidemic. However, the impact of population density on the COVID-19 outbreak in Malaysia is still unknown and undiscovered. Therefore, this study investigates the impact of population density on COVID-19 as a potential virus transmission carrier using linear regression models. The chances of formulating new strategies for combating COVID-19 are higher when the driver of transmission potential is identified. This study shows that the highest value of infected area density is in Kota Bharu (0.76), while the infected risk area was highest in Jeli (0.33). This study found that there is a strong relationship between COVID-19 infection cases in Kelantan and population density (R2 which is 0.845). Therefore, high population density was identified as a potential driver of transmission of COVID-19 outbreak. Understanding the potential drivers of the disease in a local setting is very important for better preparation and management. The outcome of the study can aid in the development of a new analytical model for strategic planning of Zero COVID-19 for securing the public health and wellness, both social and economic, by researchers, scientists, planners, resource managers, and decision-makers