Loquat (Eriobotrya japonica) extracts suppress the adhesion, migration and invasion of human breast cancer cell line

We examined the inhibitory effects of loquat methanol extract on the adhesion, migration, invasion and matrix metalloproteinase (MMP) activities of MDA-MB-231 human breast cancer cell line. Cells were cultured with DMSO or with 10, 25, or 50 µg/ml of loquat methanol extract. Both leaf and seed extracts significantly inhibited growth of MDA-MB-231 cells in a dose-dependent manner, although leaf extract was more effective. Adhesion and migration were significantly inhibited by loquat extracts in a dose-dependent manner. Loquat extract also inhibited the invasion of breast cancer cells in a dose-dependent manner and leaf extract was more effective than seed extract. MMP-2 and MMP-9 activities were also inhibited by loquat extract. Our results indicate that methanol extracts of loquat inhibit the adhesion, migration and invasion of human breast cancer cells partially through the inhibition of MMP activity and leaf extract has more anti-metastatic effects in cell based assay than seed extract. Clinical application of loquat extract as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis

The Cherenkov Telescope Array Large Size Telescope

The two arrays of the Very High Energy gamma-ray observatory Cherenkov Telescope Array (CTA) will include four Large Size Telescopes (LSTs) each with a 23 m diameter dish and 28 m focal distance. These telescopes will enable CTA to achieve a low-energy threshold of 20 GeV, which is critical for important studies in astrophysics, astroparticle physics and cosmology. This work presents the key specifications and performance of the current LST design in the light of the CTA scientific objectives.Comment: 4 pages, 5 figures, In Proceedings of the 33rd International Cosmic Ray Conference (ICRC2013), Rio de Janeiro (Brazil). All CTA contributions at arXiv:1307.223

Machine Learning Methods for Prediction of CDK-Inhibitors

Progression through the cell cycle involves the coordinated activities of a suite of cyclin/cyclin-dependent kinase (CDK) complexes. The activities of the complexes are regulated by CDK inhibitors (CDKIs). Apart from its role as cell cycle regulators, CDKIs are involved in apoptosis, transcriptional regulation, cell fate determination, cell migration and cytoskeletal dynamics. As the complexes perform crucial and diverse functions, these are important drug targets for tumour and stem cell therapeutic interventions. However, CDKIs are represented by proteins with considerable sequence heterogeneity and may fail to be identified by simple similarity search methods. In this work we have evaluated and developed machine learning methods for identification of CDKIs. We used different compositional features and evolutionary information in the form of PSSMs, from CDKIs and non-CDKIs for generating SVM and ANN classifiers. In the first stage, both the ANN and SVM models were evaluated using Leave-One-Out Cross-Validation and in the second stage these were tested on independent data sets. The PSSM-based SVM model emerged as the best classifier in both the stages and is publicly available through a user-friendly web interface at http://bioinfo.icgeb.res.in/cdkipred

Characteristics of transport in electron internal transport barriers and in the vicinity of rational surfaces in the Large Helical Device

Characteristics of transport in electron internal transport barriers (ITB) and in the vicinity of a rational surface with a magnetic island are studied with transient transport analysis as well as with steady state transport analysis. Associated with the transition of the radial electric field from a small negative value (ion-root) to a large positive value (electron-root), an electron ITB appears in the Large Helical Device [M. Fujiwara et al., Nucl. Fusion 41, 1355 (2001)], when the heating power of the electron cyclotron heating exceeds a power threshold. Transport analysis shows that both the standard electron thermal diffusivity, chie, and the incremental electron thermal diffusivity, chieinc (the derivative of normalized heat flux to temperature gradient, equivalent to heat pulse chie), are reduced significantly (a factor 5 10) in the ITB. The chieinc is much lower than the chie by a factor of 3 just after the transition, while chieinc is comparable to or even higher than chie before the transition, which results in the improvement of electron transport with increasing power in the ITB, in contrast to its degradation outside the ITB. In other experiments without an ITB, a significant reduction (by one order of magnitude) of chieinc is observed at the O-point of the magnetic island produced near the plasma edge using error field coils. This observation gives significant insight into the mechanism of transport improvement near the rational surface and implies that the magnetic island serves as a poloidally asymmetric transport barrier. Therefore the radial heat flux near the rational surface is focused at the X-point region, and that may be the mechanism to induce an ITB near a rational surface