Short communication. Collection and characterisation of a population of Triticum boeoticum Boiss., a wild wheat species not previously found in the Mediterranean western region

En julio del 2010 se recolectó una población de trigo silvestre en una zona abandonada cerca de Madrid, España. Esta zona posee una biodiversidad botánica elevada y un tipo de suelo muy peculiar denominado “arcillas verdes”. Se recogió una muestra de trigo y se multiplicó y caracterizó para varios caracteres agro-morfológicos y subunidades de gluteninas. El número cromosómico 2n de las semillas demostró que es una especie diploide de trigo y los datos de caracterización indicaron que es Triticum boeoticum Boiss. Esta especie llegó probablemente como mala hierba del cultivo de escaña que se producía en la zona hasta al menos la primera mitad del s. xix. Las características edáficas y climáticas del lugar y el hecho de que no haya referencias hasta ahora de esta especie en la zona oeste de la región Mediterránea aumentan el valor de esta adquisición para la mejora del trigo. La nueva accesión se conserva en el Centro Nacional de Recursos Fitogenéticos y se ha depositado una hoja de herbario en el Real Jardín Botánico de Madri

Closing the cycle for the cut rose industry by the reuse of its organic wastes: A case study in Ecuador

The soil conditions and the yield and quality of Rosa sp. var. Freedom were determined following the incorporation into the soil of rose waste composts, with or without fertigation; the effects of these treatments were compared with those of non-stabilised chopped rose wastes þ fertigation (FWF). The growing conditions were those of a commercial greenhouse. The use of the composts, alone or combined with fertigation, increased the available P and K contents of the soil with respect to FWF. However, only the compost þ fertigation treatments improved, in general, the soil fertility regarding the organic matter (OM), nitrogen and available micronutrient concentrations, in comparison to FWF. When the composts were added alone, irrigation with alkaline water increased the soil pH and, in consequence, reduced the availability of micronutrients. Overall, the combined use of compost and fertigation increased the cut rose yield and quality relative to the application of compost alone and FWF. Principal component analysis indicated that the OM, available Cu, Mn and Zn and total N contents and the pH of the soil were the principal soil parameters determining the yield and quality of the roses. This analysis classified the treatments in three groups: the compost þ fertigation treatments; the treatments with compost alone; and the FWF treatment. The compost þ fertigation treatments gave the highest net income (average for these treatments ¼ 80388.92 US dollars ha 1). Therefore, the compost þ fertigation treatments were highly beneficial with regard to increasing soil fertility and cut rose yield, quality and profitability

Towards optimization-safe systems: analyzing the impact of undefined behavior

This paper studies an emerging class of software bugs called optimization-unstable code: code that is unexpectedly discarded by compiler optimizations due to undefined behavior in the program. Unstable code is present in many systems, including the Linux kernel and the Postgres database. The consequences of unstable code range from incorrect functionality to missing security checks. To reason about unstable code, this paper proposes a novel model, which views unstable code in terms of optimizations that leverage undefined behavior. Using this model, we introduce a new static checker called Stack that precisely identifies unstable code. Applying Stack to widely used systems has uncovered 160 new bugs that have been confirmed and fixed by developers.United States. Defense Advanced Research Projects Agency (DARPA Clean-slate design of Resilient, Adaptive, Secure Hosts (CRASH) program under contract #N66001-10-2-4089)National Science Foundation (U.S.) (NSF award CNS-1053143

Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases

Phytocystatins are inhibitors of cysteine-proteases from plants putatively involved in plant defence based on their capability of inhibit heterologous enzymes. We have previously characterised the whole cystatin gene family members from barley (HvCPI-1 to HvCPI-13). The aim of this study was to assess the effects of barley cystatins on two phytophagous spider mites, Tetranychus urticae and Brevipalpus chilensis. The determination of proteolytic activity profile in both mite species showed the presence of the cysteine-proteases, putative targets of cystatins, among other enzymatic activities. All barley cystatins, except HvCPI-1 and HvCPI-7, inhibited in vitro mite cathepsin L- and/or cathepsin B-like activities, HvCPI-6 being the strongest inhibitor for both mite species. Transgenic maize plants expressing HvCPI-6 protein were generated and the functional integrity of the cystatin transgene was confirmed by in vitro inhibitory effect observed against T. urticae and B. chilensis protein extracts. Feeding experiments impaired on transgenic lines performed with T. urticae impaired mite development and reproductive performance. Besides, a significant reduction of cathepsin L-like and/or cathepsin B-like activities was observed when the spider mite fed on maize plants expressing HvCPI-6 cystatin. These findings reveal the potential of barley cystatins as acaricide proteins to protect plants against two important mite pests

Los sistemas de pago por servicios ambientales entre la adicionalidad y la subsidiariedad: aplicación a la belleza escénica en el pantano de San Juan, Madrid, España

A payment for environmental services (PES) based on the beauty of the San Juan dam landscape is proposed containing two innovative aspect in regard to previous PES systems: the elaboration of a diagnosis oriented to obtain a shared vision of the area based in a participative process including all relevant local stakeholders, and the integration of the PES mechanism into the regional public environmental policy. The proposed system also identifies the offer and the demand of the environmental service. It shows the main problems associated to the preservation of the service and proposes management plans oriented to its conservation. Finally, the economic viability of the system is studied comparing three different scenarios according to the grade of integration of the PES model into the regional conservation public policy. This paper shows how a PES system can be used to guarantee public investments in nature conservation through a private management.Se plantea un sistema de pago por servicios ambientales (PSA) basado en la valoración de la belleza escénica en la zona del pantano de San Juan, Madrid, con dos aspectos novedosos respecto a estudios PSA previos: la elaboración de un diagnóstico basado en un proceso participativo que involucra a todos los actores sociales locales y cuyo fin es el de obtener una visión compartida sobre el área, y la integración del mecanismo PSA en la gestión pública regional de la conservación de la naturaleza. El sistema propuesto además identifica los oferentes y demandantes del servicio ambiental, muestra los principales problemas asociados a la conservación del servicio y plantea programas de gestión orientados a contribuir a su conservación. Se estudia, por último, la viabilidad económica del sistema comparando tres escenarios hipotéticos en función del grado de integración del sistema PSA en las políticas públicas de conservación de la naturaleza regionales. El trabajo muestra como un sistema PSA puede servir para garantizar las inversiones públicas en conservación a través de la gestión privada

Estrogenicity of resin-based composites and sealants used in dentistry.

We tested some resin-based composites used in dentistry for their estrogenic activity. A sealant based on bisphenol-A diglycidylether methacrylate (bis-GMA) increased cell yields, progesterone receptor expression, and pS2 secretion in human estrogen-target, serum-sensitive MCF7 breast cancer cells. Estrogenicity was due to bisphenol-A and bisphenol-A dimethacrylate, monomers found in the base paste of the dental sealant and identified by mass spectrometry. Samples of saliva from 18 subjects treated with 50 mg of a bis-GMA-based sealant applied on their molars were collected 1 hr before and after treatment. Bisphenol-A (range 90-931 micrograms) was identified only in saliva collected during a 1-hr period after treatment. The use of bis-GMA-based resins in dentistry, and particularly the use of sealants in children, appears to contribute to human exposure to xenoestrogens

Global potential energy surface for the O2 + N2 interaction. Applications to the collisional, spectroscopic, and thermodynamic properties of the complex

A detailed characterization of the interaction between the most abundant molecules in air is important for the understanding of a variety of phenomena in atmospherical science. A completely {\em ab initio} global potential energy surface (PES) for the O$_2(^3\Sigma^-_g)$ + N$_2(^1\Sigma^+_g)$ interaction is reported for the first time. It has been obtained with the symmetry-adapted perturbation theory utilizing a density functional description of monomers [SAPT(DFT)] extended to treat the interaction involving high-spin open-shell complexes. The computed interaction energies of the complex are in a good agreement with those obtained by using the spin-restricted coupled cluster methodology with singles, doubles and noniterative triple excitations [RCCSD(T)]. A spherical harmonics expansion containing a large number of terms due to the anisotropy of the interaction has been built from the {\em ab initio} data. The radial coefficients of the expansion are matched in the long range with the analytical functions based on the recent {\em ab initio} calculations of the electric properties of the monomers [M. Bartolomei et al., J. Comp. Chem., {\bf 32}, 279 (2011)]. The PES is tested against the second virial coefficient $B(T)$ data and the integral cross sections measured with rotationally hot effusive beams, leading in both cases to a very good agreement. The first bound states of the complex have been computed and relevant spectroscopic features of the interacting complex are reported. A comparison with a previous experimentally derived PES is also provided