Basal and ?-Adrenergic cardiomyocytes contractility dysfunction induced by dietary protein restriction is associated with downregulation of SERCA2a expression and disturbance of endoplasmic reticulum Ca2+ regulation in rats.

Background: The mechanisms responsible for the cardiac dysfunction associated with dietary protein restriction (PR) are poorly understood. Thus, this study was designed to evaluate the effects of PR on calcium kinetics, basal and ?-adrenergic contractility in murine ventricular cardiomyocytes. Methods: After breastfeeding male Fisher rats were distributed into a control group (CG, n = 20) and a protein-restricted group (PRG, n = 20), receiving isocaloric diets for 35 days containing 15% and 6% protein, respectively. Biometric and hemodynamic variables were measured. After euthanasia left ventricles (LV) were collected for histopathological evaluation, SERCA2a expression, cardiomyocytes contractility and Ca2+ sparks analysis. Results: PRG animals showed reduced general growth, increased heart rate and arterial pressure. These animals presented extracellular matrix expansion and disorganization, cardiomyocytes hypotrophy, reduced amplitudes of shortening and maximum velocity of contraction and relaxation at baseline and after ?-adrenergic stimulation. Reduced SERCA2a expression as well as higher frequency and lower amplitude of Ca2+ sparks were observed in PRG cardiomyocytes. Conclusion: The observations reveal that protein restriction induces marked myocardial morphofunctional damage. The pathological changes of cardiomyocyte mechanics suggest the potential involvement of the ?-adrenergic system, which is possibly associated with changes in SERCA2a expression and disturbances in Ca2+ intracellular kinetics

Geo-environmental management of watersheds : the geological systems as the initial decision to land use.

Ap?s a cartografia da bacia do Rio Paracatu (Noroeste de Minas Gerais) ser atualizada em escala de 1:250.000, foi poss?vel elaborar a l?gica para a metodologia de gest?o geoambiental/geossistemas, como parte de uma ampla metodologia de gest?o, em elabora??o desde 1998. Apresenta-se como primeira etapa a verifica??o das rela??es entre rochas, geoformas e solos com as quais se exploram possibilidades e potencialidades de usos da terra para produ??o agr?cola, florestal, industrial, rural, energ?tica e de obras civis. Com o quadro de potencialidades fundamentado sobre os geossistemas, foi poss?vel ent?o realizar uma an?lise avaliativa das condi??es de acerto ou inconveni?ncia dos usos atuais da terra, as possibilidades de expans?o de usos e as necessidades de mitiga??o sob o ponto de vista da estabilidade do substrato. Com a cartografia na escala de 1:250.000 pode-se estabelecer uma vis?o regional e fornecer indicativos para estudos em escala local e a tomada de decis?o que envolve ainda um conjunto de outras ci?ncias, engenharias e modelos econom?tricos. Este artigo apresenta os procedimentos dessa primeira etapa de aux?lio ? elabora??o de decis?es, passo a passo.After the mapping of the Paracatu River basin (Northwestern Minas Gerais, Brazil) was updated on a scale of 1:250,000, it was possible to elaborate the rationale for the methodology of geo-environmental/geosystems management as part of a broad management methodology, under development since 1998. It presents as the first step to verify the relationship between rocks, landforms, and soils with which one can explore possibilities and potential land use for agricultural production, forestry, rural industrial, energy production, and civil works. With the framework of potentials based on geosystems, it was possible to make an evaluation analysis about the terms of settlement or inconvenience of the land current uses, the possibilities for expansion of uses, and the requirements for mitigation under the terms of the substrate stability. With the mapping at the 1:250,000 scale, it was possible to establish a regional vision provide the indicative scale studies and local decision-making that still involves a number of other sciences, engineering as well as the econometric models. This article presented the procedures of the first stage of the aid business decision making, step-by-step

Measurement of the inclusive jet cross section in pp collisions at √s=2.76 TeV

The double-differential inclusive jet cross section is measured as a function of jet transverse momentum pT and absolute rapidity IyI, using proton-proton collision data collected with the CMS experiment at the LHC, at a center-of-mass energy of √s=2.76 TeV and corresponding to an integrated luminosity of 5.43 pb⁻¹. Jets are reconstructed within the pT range of 74 to 592 GeV and the rapidity range IyI < 3.0.The reconstructed jet spectrum is corrected for detector resolution. The measurements are compared to the theoretical prediction at next-to-leading-order QCD using different sets of parton distribution functions. This inclusive cross section measurement explores a new kinematic region and is consistent with QCD predictions

Aquifer recharge zones and ecologic-economic conservational solutions with agroforestry projects.

Geoci?ncias agr?rias e ambientais (GAA) implicam em integrar diversas ci?ncias e conhecimentos que subsidiem a pr?tica da agricultura, silvicultura e zoocultura, de modo conservacionista, no ?mbito da gest?o de bacia hidrogr?fica. Uma das ?reas protegidas por lei, mas comumente ocupadas, inclusive por desconhecimento de onde se situam nas muitas bacias, s?o as zonas de recarga de aq??feros. S?o ?reas sens?veis, nas quais uma simples proibi??o de atividades agr?colas pode n?o ser produtivo, visto que existem solu??es ecol?gico-econ?micas que seriam n?o somente vi?veis quanto desej?veis. A integra??o de conhecimentos em Geologia estrutural, Hidrogeologia, Geomorfologia, Geotecnia, Pedologia, Aptid?o de solos, Agronomia, Engenharia florestal, Lito-estratigrafia comp?e um dos quadros de conhecimentos das GAA. Alguns aspectos da l?gica de integra??o desses conhecimentos ? aqui exposta de modo a se evidenciar esse novo ramo das Geoci?ncias. A Bacia do Rio Paracatu ? alvo de estudo por ser uma regi?o de frente agr?cola intensiva. Os conceitos de seguran?a qu?mica, seguran?a do terreno e de uso agro-florestal intensivo e/ou org?nico comp?em o quadro l?gico para o aux?lio ? decis?o na gest?o de bacia. Decidir simplesmente com a aptid?o de solos ? limitado do ponto de vista geo-ambiental. Nesse trabalho, essa limita??o ? proposta a ser ultrapassada com um sistema de aux?lio ? decis?o com uso de Intelig?ncia artificial.Agrarian Geosciences as a proposed new branch of knowledge implies the integration of various sciences, which serve to agriculture, forestry and animal breeding practices with conservation procedures for watersheds management. Underground water recharge zones are legally protected areas, but usually occupied for agricultural purposes given the general misinformation on their real localizations. They are sensible zones, where the very simple prohibition of agricultural activities may not be a productive approach meanwhile there are quite a good number of ecologic-economic solutions, which are viable and desirable. Knowledge procedures for integrating Structural Geology, Hydrogeology, Geomorphology, Geothenics, Pedology, Land sustainability, Litostratigraphy, Agronomy and Forestry engineering are parts of a knowledgeable approach to Agrarian Geosciences. A logical approach to integrate all this knowledge is presented in such a way that the Agrarian Geosciences may become evident to the reader. Paracatu watershed is the studied region with intensive agricultural projects. The concepts of chemical security, land sustainability, and the possibility of intensive agro-forestry projects as well as organic projects are part of a logic approach for helping decision making. To decide on the bases of soil sustainability, though absolutely correct from the agricultural point of view is a limited approach from a larger geo-environmental point of view. In this paper, these limitations are proposed to be partly overcome with artificial intelligent systems for supporting decision making

Protein restriction after weaning modifies the calcium kinetics and induces cardiomyocyte contractile dysfunction in rats.

Protein restriction (PR) is associated with cardiovascular diseases. The purpose of this study was to investigate the effects on single ventricular cardiomyocyte contractile function of a short-term PR after weaning. Male Fischer rats that were 28 days old were randomly divided into a control group (CG, n = 16) and a protein-restricted group (PRG, n = 16). After weaning, CG and PRG animals received isocaloric diets containing 15 and 6% protein, respectively, for 35 days. Biometric parameters were then measured, and the hearts were removed for the analysis of contractile function and calcium transient in isolated cardiomyocytes of the left ventricule (LV), and the quantification of calcium and collagen fibers in LV myocardium. PRG animals had lower body weight (BW) and LV weight (LVW), an increased LVW to BW ratio and a higher proportion of collagen fibers than CG animals. PRG animals exhibited reduced tissue levels of calcium, reduced the length, width and volume of cardiomyocytes and their sarcomere length compared to CG animals. Cardiomyocytes from PRG animals had a lower amplitude of shortening, a slower time to the peak of shortening and a longer time to half-relaxation than those from the CG. Cardiomyocytes from PRG animals also presented a lower peak of calcium transient and a longer calcium transient decay time than CG animals. Taken together, the results indicate that short-term PR after weaning induces a marked structural remodeling of the myocardium parenchyma and stroma that coexists with contractile dysfunctions in single LV cardiomyocytes of rats, which is probably associated with pathological changes of the intracellular calcium kinetics, rather than inadequate available amounts of this mineral in cardiac tissue

Measurement of the CP-violating phase phi_s in the Bs0→^0_\mathrm{s}\to J/ψ ϕ/\psi\, \phi(1020) →μ+μ−\to \mu^+\mu^-K+^+K−^- channel in proton-proton collisions at s=\sqrt{s} = 13 TeV

The CP-violating weak phase ?s and the decay width difference ??s between the light and heavy B0s mass eigenstates are measured with the CMS detector at the LHC in a sample of 48 500 reconstructed B0s? J/I) d (1020) ?11+11? K+K? events. The measurement is based on a data sample corresponding to an integrated luminosity of 96.4 fb?1, collected in proton-proton collisions at ?s = 13 TeV in 2017?2018. To extract the values of ?s and ??s, a time-dependent and flavor-tagged angular analysis of the 11+11?K+K? final state is performed. The analysis employs a dedicated tagging trigger and a novel opposite-side muon flavor tagger based on machine learning techniques. The measurement yields ?s = ?11 ?50 (stat) ? 10 (syst) mrad and ??s = 0.114 ? 0.014 (stat)? 0.007 (syst) ps?1, in agreement with the standard model predictions. When combined with the previous CMS measurement at ?s = 8 TeV, the following values are obtained: ?s = ?21 ? 44 (stat) ? 10 (syst) mrad, ??s = 0.1032 ? 0.0095 (stat) ? 0.0048 (syst) ps?1, a significant improvement over the 8 TeV result. ? 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY licens

Measurement of the C P-violating phase φs in the B0 s → J/ψφ(1020) → μ+μ− K+K− channel in proton-proton collisions at √s = 13 TeV

The C P-violating weak phase φs and the decay width difference s between the light and heavy B0 s mass eigenstates are measured with the CMS detector at the LHC in a sample of 48 500 reconstructed B0 s → J/ψ φ(1020) → μ+μ− K+K− events. The measurement is based on a data sample corresponding to an integrated luminosity of 96.4 fb−1, collected in proton-proton collisions at √s = 13 TeV in 2017–2018. To extract the values of φs and s, a time-dependent and flavor-tagged angular analysis of the μ+μ−K+K− final state is performed. The analysis employs a dedicated tagging trigger and a novel opposite-side muon flavor tagger based on machine learning techniques. The measurement yields φs = −11±50 (stat)±10 (syst) mrad and s = 0.114±0.014 (stat)±0.007 (syst) ps−1, in agreement with the standard model predictions. When combined with the previous CMS measurement at √s = 8 TeV, the following values are obtained: φs = −21 ± 44 (stat) ± 10 (syst) mrad, s = 0.1032 ± 0.0095 (stat) ± 0.0048 (syst) ps−1, a significant improvement over the 8 TeV result

Search for heavy resonances decaying to WW, WZ, or WH boson pairs in a final state consisting of a lepton and a large-radius jet in proton-proton collisions at s =13 TeV

A search for new heavy resonances decaying to pairs of bosons (WW, WZ, or WH) is presented. The analysis uses data from proton-proton collisions collected with the CMS detector at a center-of-mass energy of 13 TeV, corresponding to an integrated luminosity of 137 fb(-1). One of the bosons is required to be a W boson decaying to an electron or muon and a neutrino, while the other boson is required to be reconstructed as a single jet with mass and substructure compatible with a quark pair from a W, Z, or Higgs boson decay. The search is performed in the resonance mass range between 1.0 and 4.5 TeVand includes a specific search for resonances produced via vector boson fusion. The signal is extracted using a twodimensional maximum likelihood fit to the jet mass and the diboson invariant mass distributions. No significant excess is observed above the estimated background. Model-independent upper limits on the production cross sections of spin-0, spin-1, and spin-2 heavy resonances are derived as functions of the resonance mass and are interpreted in the context of bulk radion, heavy vector triplet, and bulk graviton models. The reported bounds are the most stringent to date

Observation of B<inf>s</inf>⁰ mesons and measurement of the B<inf>s</inf>⁰/B⁺ yield ratio in PbPb collisions at [Formula presented] TeV

The Bs0 and B+ production yields are measured in PbPb collisions at a center-of-mass energy per nucleon pair of 5.02 TeV. The data sample, collected with the CMS detector at the LHC, corresponds to an integrated luminosity of 1.7 nb−1. The mesons are reconstructed in the exclusive decay channels B→s0J/ψ(μ+μ−)ϕ(K+K−) and B→+J/ψ(μ+μ−)K+, in the transverse momentum range 7–50 GeV/c and absolute rapidity 0–2.4. The Bs0 meson is observed with a statistical significance in excess of five standard deviations for the first time in nucleus-nucleus collisions. The measurements are performed as functions of the transverse momentum of the B mesons and of the PbPb collision centrality. The ratio of production yields of Bs0 and B+ is measured and compared to theoretical models that include quark recombination effects