Investigation and identification of helminth parasites in five fish species of Anzali Wetland

This study aimed to identify of helminth parasites in some native and economic fish of Anzali wetland was performed. 314 fish specimens, including: Alburnus hohenackeri (60 Specimen), Blicca bjoerkna (75 Specimen), Hemiculter leucisculus (75 Specimen), Pseudorasbora parva (52 pcs) Rhodeus amarus (52 Specimen), from Cyprinidae (Cyprinidae), during 1 year (2012-2013) and seasonally, from three different stations (Shijan, Pirbazar and Pasikhan) via gill net (50-70 mm) and fyke net were sampled. Samples were transported to the Inlandwater Aquaculture Institute in Bandar Anzali. In the laboratory, After biometry and determine the age and sex of fish, different body parts for searching of parasites were checked and parasites by using identification keys were identified. As a result of this study, 9 species of helminth parasites of fishes were identified include: Gyrodactylus sp., Dactylogyrus sphyrna , Dactylogyrus sp., Ligula intestinalis, Diplostomum paraspathaceum, Diplostomum spathaceum, Diplostomum sp., Posthodiplostomum cuticola and Rhabdochona denudate. The highest frequency of parasites in trematodes (in 5 fish species studied) and the minimum frequency in cestodes (each at 1 fish species studied) were seen. According to statistical analysis using Chi-Square test and KruskalWallis, the pollution of the sex, weight, length and sampling stations, there is no statistically significant difference (P> 0.05)

Validity and reliability of short-form : 12 questionnaire in Iranian hemodialysis patients

Introduction. The aim of the study was to assess the validity and reliability of the SF-12 questionnaire in a sample of Iranian patients undergoing hemodialysis. Materials and Methods. One hundred and forty-four hemodialysis patients were included from dialysis centers in Zanjan, Iran, and were asked to complete the SF-12 and SF-36 questionnaires. An initial test-retest reliability evaluation was performed on a sample of 70 patients from the total group, with a retest interval of 14 days. Reliability was estimated by internal consistency and validity was assessed using known-group comparisons and construct validity on the patient group as a whole. A linear regression analysis was used to assess any variation in the physical component summary and mental component summary scores of the SF-36 with the respective component summary scores of the SF-12. In addition, the factor structure of the questionnaire was extracted by performing a confirmatory factor analysis. Results. Cronbach alpha for physical and mental component summaries were 0.89 and 0.90, respectively. The SF-12 showed a good discriminatory ability between subgroups of patients based on demographic and clinical variables. The confirmatory factor analysis for the original two-factor structure showed a good fit index (chi2 = 23.30, degrees of freedom = 13), goodness-of-fit index = 0.96, and root mean squared error of approximation = 0.079). Conclusions. In general, the SF-12 has good psychometric properties and can be used as a shorter version of the SF-36 questionnaire in future studies involving Iranian patients undergoing hemodialysis