VIRULENSI BAKTERI Edwardsiella ictaluri PENYEBAB PENYAKIT ENTERIC SEPTICEMIA OF CATFISH (ESC) PADA IKAN PATIN (Pangasius pangasius)

Penyakit infeksius yang menyerang ikan patin, salah satunya disebabkan bakteri Edwardsiella ictaluri.  Bakteri ini menyebabkan penyakit sistemik enteric septicemia of catfish (ESC). Penelitian bertujuan untuk mengetahui gejala klinis dan rerata waktu kematian akibat serangan bakteri E. ictalury pada ikan patin. Bakteri diinokulasi ke media BHIA (Brain Heart Infusion Agar, merek Conda Pronadisa) dan BHIB (Brain Heart Infusion Broth, merek Merck) diinkubasi pada suhu 24 oC selama 24 - 48 jam. Suspensi bakteri dengan kepadatan 2,7 x 101 – 1010 cfu/mL diinfeksikan pada 300 ekor ikan patin berukuran 11-12 cm dan diamati selama 7 hari. Penelitian menggunakan metode eksperimental dan Completely Randomized Design (CRD). Analisis lebih lanjut dengan Duncan multiple range test (DMRT). Hasil Penelitian menunjukkan gejala klinis yang muncul pada ikan patin antara lain, ikan berenang secara vertikal, warna tubuh pucat, nekrosis, petechiae, liver pucat dan membesar, ginjal berwarna gelap dan kaku, asites insang pucat. Nilai rerata waktu kematian (RWK) ikan patin berkisar antara 1,16 – 2,26 hari.   Infectious diseases that attack catfish, one of which is caused by the bacterium Edwardsiella ictaluri. This bacterium causes systemic enteric septicemia of catfish (ESC). The aim of the study was to determine the clinical symptoms and the mean time of death due to the attack of E. ictaluri bacteria on catfish. Bacteria were inoculated into BHIA media (Brain Heart Infusion Agar, Conda Pronadisa brand) and BHIB (Brain Heart Infusion Broth, Merck brand) were incubated at 24 oC for 24-48 hours. Bacterial suspension with a density of 2.7 x 101 – 1010 cfu/mL was infected with 300 catfish measuring 11-12 cm and observed for 7 days. The study used experimental methods and completely randomized design (CRD). Further analysis with Duncan multiple range test (DMRT). The results showed clinical symptoms that appeared in catfish, among others, fish swimming vertically, pale body color, necrosis, petechiae, pale and enlarged liver, dark and stiff kidneys, ascites, pale gills. The mean time of death (MTD) of catfish ranged from 1.16 to 2.26 days

Users’ preferences on the provision of facilities for mass rapid transit Sungai Buloh – Subang - Putrajaya line (MRT2) stations

National Transport Policy (2019-2030) strives to improve the transportation sector towards a conducive and competitive sector while fulfils the demands of people. The new development of Mass Rapid Transit (MRT2) Sungai Buloh – Subang – Putrajaya Line is expected to complete in 2022 and would benefit 529,000 users. This paper analyses the preferences of users on the facility provision at mass rapid transit stations in the local context. This paper was based on secondary and primary data sources such as official documents, websites and data collected for the Integrated MRT2 Land Use Masterplan. Document analysis method was employed to review official sources from public transport providers across selected benchmarked countries. Six categories of facilities at mass rapid transit stations were evaluated using 40 criteria. Results suggested improvements are needed in barrier-free considerations and convenient facilities. Statistical analyses, involving descriptive and inferential analyses, were conducted. The results show that gender and travel time to station influenced users’ preferences on facilities needed at stations. This paper suggests that certain criteria of facilities must be considered based on universal needs of the population to encourage usage of MRT2 as public transport as well as to provide convenient travel experiences

Assessing the current implementation of transit oriented development concept in Malaysia: Case study of MRT Putrajaya line

In the past years, the concept of Transit-Oriented Development has been adopted in cities and countries including Malaysia and Singapore. The integration of land use and public transport stations through Transit-Oriented Development (TOD) as part of urban and cities strategy is highly acclaimed in promoting sustainable development concept in cities development. To understand the performance of TODs implementation in Klang Valley, this study has selected eleven stations in of Mass Rapid Transit (MRT) Putrajaya Line as case studies. This paper aims to evaluate the current implementation of TODs in Malaysia, benchmarked against the TOD land use composition and percentage from Singapore’s model. The findings show that Raja Uda, Ampang Park and Persiaran KLCC stations show significant performance. However, Bandar Malaysia North station displayed poor results with the lowest percentage of residential and commercial components. All stations did not achieve the ideal TOD value for residential land use and eight out of eleven stations recorded higher than the ideal TOD value for roads. However, highest number of stations achieved the ideal TOD value for mixed-use and commercial land uses. These results would help policymakers to improve the current implementation of TODs in Malaysia

Hardware Trojan Identification Using Machine Learning-based Classification

As Hardware Trojans (HTs) emerges as the new threats for the integrated circuits (ICs), methods for identifying and detecting HTs have been widely researched and proposed. Identifying the HTs are important because it can assist in developing proper techniques for inserting and detecting the treat in ICs. One of the recent method of identifying and detecting HTs in ICs is classification using machine learning (ML) algorithm. There is still lack of machine learning-based classification for HTs identification. Thus, a three type of ML based classification includes Decision Tree (DT), K-Nearest Neighbor (KNN) and Support Vector Machine (SVM) are proposed for HTs identification. The dataset is based from the Trust-Hub. In order to improve the classification accuracy, the HTs are discretized based on their dominant attributes. The discretized HTs are classified using three machine learning algorithms. The results show that the DT and KNN learnt model are able to correctly predict about 83% of the test data

A comparative analysis of transit oriented development guidelines between Malaysia and other countries

Transit Oriented Development (TOD) is an emerging concept to optimise the land use development surrounding a transit station and to create a reliable relationship between a densely compact urban form and high public transportation ridership. In Malaysia, the concept of TOD was initiated in the first National Physical Plan in 2005 and mentioned again in National Physical Plan 2 in 2010. This paper identifies the principle of TOD applied in Malaysia and other countries, and discusses the differences and similarities of the TOD practices using comparative analysis and document analysis methods. Findings of this study indicate that mixed-use development, high density, intensity and connectivity are the main TOD principles adopted in Malaysia and other countries around the globe. The current policy and practises of these countries tend to focus more on the better management and increase ridership of these transit rail stations, including ways to encourage users to shift from private vehicles to public transportation. The findings of this study would contribute towards policy decisions and practices of TOD in Malaysia. © 2021 Malaysian Institute Of Planners. All rights reserved

Phytoconstituents of <i>Endiandra kingiana</i>; antidiabetic effects and molecular docking studies on alpha-amylase and alpha-glucosidase

Phytochemical investigation on the bark of E. kingiana plant afforded ten compounds, including six polyketides namely kingianin A 1, kingianin B 2, kingianin E 3, kingianin F 4, kingianin K 5 and kingianin L 6, three endiandric acids; kingianic acid A 7, tsangibeilin B 8 and endiandric acid M 9, and one sesquiterpene; daibuoxide 10. All compounds were separated as racemic mixture by recycling high-performance liquid chromatography (RHPLC), except for daibuoxide. Their structures were elucidated by detailed spectroscopic and comparative literature data analysis. This is the first report on the presence of the sesquiterpene; daibuoxide in Endiandra genus. In vitro enzymatic bio-evaluation of the isolated compounds against α-amylase and α-glucosidase showed that 4 demonstrated the best α-amylase and α-glucosidase inhibitory activity with IC50 values of 181.54 ± 6.27 µg/mL and 237.87 ± 0.07 µg/mL, respectively. In addition, molecular docking analysis confirmed the α-amylase and α-glucosidase inhibitory activities demonstrated by 4.</p