Clarification of factors that affect the flux performance of hollow fiber membranes during ultrafiltration using design of experiments

In this paper, the separation of humic substances from oily wastewater was investigated using Hollow Fiber membranes. Consideration was given to the increse of membrane permeability or flux of the Ultrafiltration process. Specifically, several factors which were temperature, pressure, time, pH and surface area of membrane, were studied. The Design of Experiments (DOE) methodology was used to investigate the effect of the factors. From the analysis of variance (ANOVA), it was determined that the pH and temperature of feed solution, time of separation process and transmembrane pressure are significant. The results of this study help to increase the permeability of membranes, thus contributing to a more sustainable filtration system

Modeling of preparation conditions of PES ultrafiltration hollow fiber membranes using statistical regression techniques

Mathematical modeling of the spinning process is crucial for a better understanding of the process variables and process functionality in membrane development. Due to the broad use and key importance of mathematical models in chemical process engineering, experimental design is becoming essential for the rapid development and validation of these empirical models. This work used the design of experiment methodology and aimed to predict the performance of ultrafiltration systems for water treatment by considering the statistical regression technique as an important approach for modeling flux. The utilization of regression modeling was also explored to show the principle elements for predicting flux in the spinning process. In order to investigate how proficient the statistical regression technique is at approximating the predicted value for flux, a real spinning experiment was conducted in this study. In this experiment, 30 samples of data were collected based on a half fractional factorial experiment with design resolution V, as well as 4 replications of center points and 10 axial points. The spinning factors that were investigated are the dope extrusion rate, air gap length, coagulation bath temperature, bore fluid ratio, and post-treatment time for predicting the corresponding flux. The regression model obtained shows that there is a correlation between the experimental data and predicted values. The results of the proposed model can be used to give a good prediction of the spinning process during membrane fabrication

Comparison of Methods for Isolating High Quality DNA and RNA from an Oleaginous Fungus Cunninghamella bainieri Strain 2a1

A number of protocols have been reported for efficient fungal DNA and RNA isolation. However, many of these methods are often designed for certain groups or morphological forms of fungi and, in some cases, are species dependent. In this report, we compared four published protocols for DNA isolation from a locally isolated oleaginous fungus, Cunninghamella bainieri strain 2a1. These protocols either involved the use of polyvinyl pyrrolidone (PVP), hexacetyltrimethylammonium bromide (CTAB) or without using PVB or CTAB. For RNA isolation, we tested two published protocols, one of which is based on TRI REAGENT (Molecular Research Center, USA) and another is simple method employing phenol for RNA extraction and LiCl for precipitation. We found that the protocol involving the use of CTAB produced the highest genomic DNA yield with the best quality compared to other protocols. In the presence of CTAB, unwanted polysaccharides were removed and this method yielded an average amount of 816 ± 12.2 µg DNA/g mycelia with UV absorbance ratios A260/280 and A260/230 of 1.67 ± 0.64 and 1.97 ± 0.23, respectively. The genomic DNA isolated via this protocol is also suitable for PCR amplification and restriction enzyme digestion. As for RNA isolation, the method involving phenol extraction and LiCl precipitation produced the highest yield of RNA with an average amount of 372 ± 6.0 µg RNA/g mycelia. The RNA appears to be relatively pure since it has UV absorbance ratios A260/280 and A260/230 of 1.89 ± 2.00 and 1.99 ± 0.03, respectively. Finally, we have demonstrated that this method could produce RNA of sufficient quality for RT-PCR that amplified a 600 bp fragment of ∆12-fatty acid desaturase gene in C. bainieri