The WISE AGN Catalog

We present two large catalogs of AGN candidates identified across ~75% of the sky from the Wide-field Infrared Survey Explorer's AllWISE Data Release. Both catalogs, some of the largest such catalogs published to date, are selected purely on the basis of mid-IR photometry in the WISE W1 and W2 bands. The catalogs are designed to be appropriate for a broad range of scientific investigations, with one catalog emphasizing reliability while the other emphasizes completeness. Specifically, the R90 catalog consists of 4,543,530 AGN candidates with 90% reliability, while the C75 catalog consists of 20,907,127 AGN candidates with 75% completeness. We provide a detailed discussion of potential artifacts, and excise portions of the sky close to the Galactic Center, Galactic Plane, nearby galaxies, and other expected contaminating sources. Our final catalogs cover 30,093 deg^2 of extragalactic sky. These catalogs are expected to enable a broad range of science, and we present a few simple illustrative cases. From the R90 sample we identify 45 highly variable AGN lacking radio counterparts in the FIRST survey, implying they are unlikely to be blazars. One of these sources, WISEA J142846.71+172353.1, is a mid-IR-identified changing-look quasar at z=0.104. We characterize our catalogs by comparing them to large, wide-area AGN catalogs in the literature, specifically UV-to-near-IR quasar selections from SDSS and XDQSOz, mid-IR selection from Secrest et al. (2015) and X-ray selection from ROSAT. From the latter work, we identify four ROSAT X-ray sources that each are matched to three WISE-selected AGN in the R90 sample within 30". Palomar spectroscopy reveals one of these systems, 2RXS J150158.6+691029, to consist of a triplet of quasars at z=1.133 +/- 0.004, suggestive of a rich group or forming galaxy cluster.(Abridged)Comment: Accepted for publication in the Astrophysical Journal Supplements. Updated with comments from the referee. 20 pages, 15 figures, 8 tables. The WISE AGN Catalogs can be made available upon request by writing to [email protected]

Antimicrobial properties of nanostructured surfaces - demonstrating the need for a standard testing methodology

Bioinspired nanostructured materials that exhibit antimicrobial properties are being synthesized and tested at increasing rates for use in healthcare, manufacturing processes, and diagnostics. Although progress has been made in improving and understanding their bactericidal activity, arguably, the biggest problem currently in the field is the lack of a standard testing methodology that allows for optimal characterization and better comparison of emerging nanostructures. Here, we examine two forms of nanostructured silicon that vary in their ability to kill certain bacterial species due to different physical mechanisms and derive guidelines for the comparative testing. We perform a comprehensive evaluation of methodologies used extensively in the field (e.g., colony counting and live dead analysis) and the novel application of high-throughput flow cytometry. The data reveal how the techniques are complementary but not always directly equivalent or correlative. Therefore, comparison of results obtained using different methodologies on different materials can be grossly misleading. We report significant variations in bactericidal efficiencies depending on experimental environments (medium type, etc.) and methodologies employed. In addition, we demonstrate how cytometry is yet another powerful complementary tool that can aid the mechanistic understanding of antimicrobial activities of rough surfaces. Besides standardization for comparison, ultimately, evaluation methods need to consider anticipated applications. Then and only then can the true potential (or limitation) of a novel material be determined for its suitability for advancement in a particular field of use

SepF supports the recruitment of the DNA translocase SftA to the Z-ring

In many bacteria, cell division begins before the sister chromosomes are fully segregated. Specific DNA translocases ensure that the chromosome is removed from the closing septum, such as the transmembrane protein FtsK in Escherichia coli. Bacillus subtilis contains two FtsK homologues, SpoIIIE and SftA. SftA is active during vegetative growth whereas SpoIIIE is primarily active during sporulation and pumps the chromosome into the spore compartment. FtsK and SpoIIIE contain several transmembrane helices, however, SftA is assumed to be a cytoplasmic protein. It is unknown how SftA is recruited to the cell division site. Here we show that SftA is a peripheral membrane protein, containing an N-terminal amphipathic helix that reversibly anchors the protein to the cell membrane. Using a yeast two-hybrid screen we found that SftA interacts with the conserved cell division protein SepF. Based on extensive genetic analyses and previous data we propose that the septal localization of SftA depends on either SepF or the cell division protein FtsA. Since SftA seems to interfere with the activity of SepF, and since inactivation of SepF mitigates the sensitivity of a ∆sftA mutant for ciprofloxacin, we speculate that SftA might delay septum synthesis when chromosomal DNA is in the vicinity

Supersymmetric structure of the induced W gravities

We derive the supersymmetric structure present in W-gravities which has been already observed in various contexts as Yang-Mills theory, topological field theories, bosonic string and chiral W_{3}-gravity. This derivation which is made in the geometrical framework of Zucchini, necessitates the introduction of an appropriate new basis of variables which replace the canonical fields and their derivatives. This construction is used, in the W_{2}-case, to deduce from the Chern-Simons action the Wess-Zumino-Polyakov action.Comment: 17 pages, Latex. To appear in Class. Quantum. Gravit

Metabolic and chromosomal changes in a <i>Bacillus subtilis whiA</i> mutant

The conserved protein WhiA is present in most Gram-positive bacteria and plays a role in cell division. WhiA contains a DNA-binding motif and is a transcription regulator of the key cell division gene ftsZ in actinomycetes. In Bacillus subtilis, the absence of WhiA influences both cell division and chromosome segregation; however, the protein does not regulate any gene involved in these processes. In this study, we addressed three alternative mechanisms by which WhiA might exert its activity in B. subtilis and examined whether WhiA influences either (i) central carbon metabolism, (ii) fatty acid composition of the cell membrane, or (iii) chromosome organization. Mutations in glycolytic enzymes have been shown to influence both cell division and DNA replication. To measure the effect of WhiA on carbon metabolism, we tested different carbon sources and measured exometabolome fluxes. This revealed that the absence of WhiA does not affect glycolysis but does influence the pool of branched-chain fatty acid precursors. Due to the effect of WhiA on chromosome segregation, we examine chromosome organization in a ∆whiA mutant using chromosome conformation capture (Hi-C) analysis. This revealed a local reduction in short-range chromosome interactions. Together, these findings provide new avenues for future research into how this protein works in the non-actinomycete firmicutes

White-gutted soldiers: simplification of the digestive tube for a non-particulate diet in higher Old World termites (Isoptera: Termitidae)

Previous observations have noted that in some species of higher termites the soldier caste lacks pigmented particles in its gut and, instead, is fed worker saliva that imparts a whitish coloration to the abdomen. In order to investigate the occurrence of this trait more thoroughly, we surveyed a broad diversity of termite specimens and taxonomic descriptions from the Old World subfamilies Apicotermitinae, Cubitermitinae, Foraminitermitinae, Macrotermitinae, and Termitinae. We identified 38 genera that have this “white-gutted” soldier (WGS) trait. No termite soldiers from the New World were found to possess a WGS caste. Externally, the WGS is characterized by a uniformly pale abdomen, hyaline gut, and proportionally smaller body-to-head volume ratio compared with their “dark-gutted” soldier (DGS) counterparts found in most termitid genera. The WGS is a fully formed soldier that, unlike soldiers in other higher termite taxa, has a small, narrow, and decompartmentalized digestive tube that lacks particulate food contents. The presumed saliva-nourished WGS have various forms of simplified gut morphologies that have evolved at least six times within the higher termites

DNA cruciform arms nucleate through a correlated but non-synchronous cooperative mechanism

Inverted repeat (IR) sequences in DNA can form non-canonical cruciform structures to relieve torsional stress. We use Monte Carlo simulations of a recently developed coarse-grained model of DNA to demonstrate that the nucleation of a cruciform can proceed through a cooperative mechanism. Firstly, a twist-induced denaturation bubble must diffuse so that its midpoint is near the centre of symmetry of the IR sequence. Secondly, bubble fluctuations must be large enough to allow one of the arms to form a small number of hairpin bonds. Once the first arm is partially formed, the second arm can rapidly grow to a similar size. Because bubbles can twist back on themselves, they need considerably fewer bases to resolve torsional stress than the final cruciform state does. The initially stabilised cruciform therefore continues to grow, which typically proceeds synchronously, reminiscent of the S-type mechanism of cruciform formation. By using umbrella sampling techniques we calculate, for different temperatures and superhelical densities, the free energy as a function of the number of bonds in each cruciform along the correlated but non-synchronous nucleation pathways we observed in direct simulations.Comment: 12 pages main paper + 11 pages supplementary dat

A First Look at Spatially Resolved Balmer Decrements at 1.0<z<2.41.0<z<2.4 from JWST NIRISS Slitless Spectroscopy

We present the first results on the spatial distribution of dust attenuation at $1.0<z<2.4$ traced by the Balmer Decrement, H$\alpha$/H$\beta$, in emission-line galaxies using deep JWST NIRISS slitless spectroscopy from the CAnadian NIRISS Unbiased Cluster Survey (CANUCS). H$\alpha$ and H$\beta$ emission line maps of emission-line galaxies are extracted and stacked in bins of stellar mass for two grism redshift bins, $1.0<z_{grism}<1.7$ and $1.7<z_{grism}<2.4$. Surface brightness profiles for the Balmer Decrement are measured and radial profiles of the dust attenuation towards H$\alpha$, $A_{\mathrm{H}\alpha}$, are derived. In both redshift bins, the integrated Balmer Decrement increases with stellar mass. Lower mass ($7.6\leqslant$Log($M_{*}$/M$_{\odot}$)$<10.0$) galaxies have centrally concentrated, negative dust attenuation profiles whereas higher mass galaxies ($10.0\leqslant$Log($M_{*}$/M$_{\odot}$)$<11.1$) have flat dust attenuation profiles. The total dust obscuration is mild, with on average $0.07\pm0.07$ and $0.14\pm0.07$ mag in the low and high redshift bins respectively. We model the typical light profiles of star-forming galaxies at these redshifts and stellar masses with GALFIT and apply both uniform and radially varying dust attenuation corrections based on our integrated Balmer Decrements and radial dust attenuation profiles. If these galaxies were observed with typical JWST NIRSpec slit spectroscopy ($0.2\times0.5^{\prime\prime}$ shutters), on average, H$\alpha$ star formation rates (SFRs) measured after slit-loss corrections assuming uniform dust attenuation will overestimate the total SFR by $6\pm21 \%$ and $26\pm9 \%$ at $1.0\leqslant z < 1.7$ and $1.7\leqslant z < 2.4$ respectively.Comment: 7 pages, 5 figures, submitted to ApJ

Annual transcriptome of a key zooplankton species, the copepod Calanus finmarchicus

The copepod Calanus finmarchicus (Crustacea, Copepoda) is a key zooplanktonic species with a crucial position in the North Atlantic food web and significant contributor to ocean carbon flux. Like many other high latitude animals, it has evolved a programmed arrested development called diapause to cope with long periods of limited food supply, while growth and reproduction are timed to take advantage of seasonal peaks in primary production. However, anthropogenic warming is inducing changes in the expected timing of phytoplankton blooms, suggesting phenological mismatches with negative consequences for the N. Atlantic ecosystem. While diapause mechanisms are mainly studied in terrestrial arthropods, specifically on laboratory model species, such as the fruit fly Drosophila, the molecular investigations of annual rhythms in wild marine species remain fragmentary. Here we performed a rigorous year-long monthly sampling campaign of C. finmarchicus in a Scottish Loch (UK; 56.45°N, 5.18°W) to generate an annual transcriptome. The mRNA of 36 samples (monthly triplicate of 25 individuals) have been deeply sequenced with an average depth of 137 ± 4 million reads (mean ± SE) per sample, aligned to the reference transcriptome, and filtered. We detail the quality assessment of the datasets and provide a high-quality resource for the investigation of wild annual transcriptomic rhythms (35,357 components) in a key diapausing zooplanktonic species

Creation and Validation of the Spanish Durum Wheat Core Collection.

Spanish wheat (Triticum spp.) landraces have a considerable polymorphism, containing many unique alleles, relative to other collections. The existence of a core collection is a favored approach for breeders to efficiently explore novel variation and enhance the use of germplasm. In this study, the Spanish durum wheat (Triticum turgidum L.) core collection (CC) was created using a population structure–based method, grouping accessions by subspecies and allocating the number of genotypes among populations according to the diversity of simple sequence repeat (SSR) markers. The CC of 94 genotypes was established, which accounted for 17% of the accessions in the entire collection. An alternative core collection (CH), with the same number of genotypes per subspecies and maximizing the coverage of SSR alleles, was assembled with the Core Hunter software. The quality of both core collections was compared with a random core collection and evaluated using geographic, agromorphological, and molecular marker data not previously used in the selection of genotypes. Both core collections had a high genetic representativeness, which validated their sampling strategies. Geographic and agromorphological variation, phenotypic correlations, and gliadin alleles of the original collection were more accurately depicted by the CC. Diversity arrays technology (DArT) markers revealed that the CC included genotypes less similar than the CH. Although more SSR alleles were retained by the CH (94%) than by the CC (91%), the results showed that the CC was better than CH for breeding purposes