Exsertion, flowering and shedding in Panicum maximum (Poaceae)

La floraison, l'exsertion et la maturation des graines ont été observées sur les panicules de quatre clones de #P. maximum$. Les relations à l'intérieur du clone et les différences entre clones ont été étudiées. Floraison, exsertion et maturation des graines sont des processus rapides (< 10 j). Les dates et les durées sont stables à l'intérieur du clone, et les conséquences sur la récolte semencière ont été soulignées. Deux types de comportement floral et leur interaction avec le "seed-set" ont été décrits. La présence de ce polymorphisme montre que ces deux comportements ont soit une "fitness" similaire, soit une fitness dépendante de l'environnement. L'agencement dans le temps de la floraison, de la maturation des graines et de l'apparition successive de plusieurs panicules sur une même talle semble être contrôlé pour minimiser la compétition. Plusieurs points corroborent cette hypothèse : l'arrêt de cette ramification sur la talle lié à un retard de plus de 10 jours entre 2 panicules évite le recouvrement dans le temps des trois processus ; à l'intérieur d'un plant, les talles les plus vigoureuses ont le plus de panicules ; plus le nombre de panicules par talle d'un clone est petit, plus sa panicule est grande. (Résumé d'auteur

Staggering of heading in Panicum maximum Jacq. : origin and regulation

Chez #Panicum maximum$, l'étalement de l'épiaison sur 2 mois, voire plus est un comportement fréquent. L'initiation des talles sur 1 mois est à l'origine de la première vague d'épiaison. Les vagues suivantes sont le résultat d'un processus de ramification paniculaire. Deux principaux systèmes de régulation interviennent durant l'épiaison : 1. une régulation rapide qui alterne les jours de sous-épiaison et de sur-épiaison; 2. une régulation mensuelle qui diminue ou augmente l'intensité de la troisième vague en fonction de l'intensité de la première vague. Ces régulations dans l'étalement des investissements reproductifs peuvent constituer une adaptation aux variations de pluviométrie en milieu tropical. (Résumé d'auteur

The WISE AGN Catalog

We present two large catalogs of AGN candidates identified across ~75% of the sky from the Wide-field Infrared Survey Explorer's AllWISE Data Release. Both catalogs, some of the largest such catalogs published to date, are selected purely on the basis of mid-IR photometry in the WISE W1 and W2 bands. The catalogs are designed to be appropriate for a broad range of scientific investigations, with one catalog emphasizing reliability while the other emphasizes completeness. Specifically, the R90 catalog consists of 4,543,530 AGN candidates with 90% reliability, while the C75 catalog consists of 20,907,127 AGN candidates with 75% completeness. We provide a detailed discussion of potential artifacts, and excise portions of the sky close to the Galactic Center, Galactic Plane, nearby galaxies, and other expected contaminating sources. Our final catalogs cover 30,093 deg^2 of extragalactic sky. These catalogs are expected to enable a broad range of science, and we present a few simple illustrative cases. From the R90 sample we identify 45 highly variable AGN lacking radio counterparts in the FIRST survey, implying they are unlikely to be blazars. One of these sources, WISEA J142846.71+172353.1, is a mid-IR-identified changing-look quasar at z=0.104. We characterize our catalogs by comparing them to large, wide-area AGN catalogs in the literature, specifically UV-to-near-IR quasar selections from SDSS and XDQSOz, mid-IR selection from Secrest et al. (2015) and X-ray selection from ROSAT. From the latter work, we identify four ROSAT X-ray sources that each are matched to three WISE-selected AGN in the R90 sample within 30". Palomar spectroscopy reveals one of these systems, 2RXS J150158.6+691029, to consist of a triplet of quasars at z=1.133 +/- 0.004, suggestive of a rich group or forming galaxy cluster.(Abridged)Comment: Accepted for publication in the Astrophysical Journal Supplements. Updated with comments from the referee. 20 pages, 15 figures, 8 tables. The WISE AGN Catalogs can be made available upon request by writing to [email protected]

Antimicrobial properties of nanostructured surfaces - demonstrating the need for a standard testing methodology

Bioinspired nanostructured materials that exhibit antimicrobial properties are being synthesized and tested at increasing rates for use in healthcare, manufacturing processes, and diagnostics. Although progress has been made in improving and understanding their bactericidal activity, arguably, the biggest problem currently in the field is the lack of a standard testing methodology that allows for optimal characterization and better comparison of emerging nanostructures. Here, we examine two forms of nanostructured silicon that vary in their ability to kill certain bacterial species due to different physical mechanisms and derive guidelines for the comparative testing. We perform a comprehensive evaluation of methodologies used extensively in the field (e.g., colony counting and live dead analysis) and the novel application of high-throughput flow cytometry. The data reveal how the techniques are complementary but not always directly equivalent or correlative. Therefore, comparison of results obtained using different methodologies on different materials can be grossly misleading. We report significant variations in bactericidal efficiencies depending on experimental environments (medium type, etc.) and methodologies employed. In addition, we demonstrate how cytometry is yet another powerful complementary tool that can aid the mechanistic understanding of antimicrobial activities of rough surfaces. Besides standardization for comparison, ultimately, evaluation methods need to consider anticipated applications. Then and only then can the true potential (or limitation) of a novel material be determined for its suitability for advancement in a particular field of use

A complementary approach to estimate the internal pressure of fission gas bubbles by SEM-SIMS-EPMA in irradiated nuclear fuels

International audienceThe behaviour of gases produced by fission is of great importance for nuclear fuel in operation. Within this context, a decade ago, a general method for the characterisation of the fission gas including gas bubbles in an irradiated UO$_2$ nuclear fuel was developed and applied to determine the bubbles internal pressure. The method consists in the determination of the pressure, over a large population of bubbles, using three techniques: SEM, EPMA and SIMS. In this paper, a complementary approach using the information given by the same techniques is performed on an isolated bubble under the surface and is aiming for a better accuracy compared to the more general measurement of gas content. SEM and EPMA enable the detection of a bubble filled with xenon under the surface. SIMS enables the detection of the gas filling the bubble. The quantification is achieved using the EPMA data as reference at positions where no or nearly no bubbles are detected

Supersymmetric structure of the induced W gravities

We derive the supersymmetric structure present in W-gravities which has been already observed in various contexts as Yang-Mills theory, topological field theories, bosonic string and chiral W_{3}-gravity. This derivation which is made in the geometrical framework of Zucchini, necessitates the introduction of an appropriate new basis of variables which replace the canonical fields and their derivatives. This construction is used, in the W_{2}-case, to deduce from the Chern-Simons action the Wess-Zumino-Polyakov action.Comment: 17 pages, Latex. To appear in Class. Quantum. Gravit

Efficacy of a new carvacrol-based product on Campylobacter jejuni in challenge test in vivo and impact on the whole caecal microbiota

Efficacy of a new carvacrol-based product on Campylobacter jejuni in challenge test in vivo and impact on the whole caecal microbiota. 6. International Conference on Poultry Intestinal Healt

The conserved C-terminus of the PcrA/UvrD helicase interacts directly with RNA polymerase

Copyright: © 2013 Gwynn et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by a Wellcome Trust project grant to MD (Reference: 077368), an ERC starting grant to MD (Acronym: SM-DNA-REPAIR) and a BBSRC project grant to PM, NS and MD (Reference: BB/I003142/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD

The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the oligomeric state of the active helicase complex, its processivity and the mechanism of unwinding. By tethering either DNA or PcrA to a microscope coverslip surface, unwinding of both linear and natural circular plasmid DNA by PcrA/RepD was followed in real-time using total internal reflection fluorescence microscopy. Visualization was achieved using a fluorescent single-stranded DNA-binding protein. The single-molecule data show that PcrA, in combination with RepD, can unwind plasmid lengths of DNA in a single run, and that PcrA is active as a monomer. Although the average rate of unwinding was similar in single-molecule and bulk solution assays, the single-molecule experiments revealed a wide distribution of unwinding speeds by different molecules. The average rate of unwinding was several-fold slower than the PcrA translocation rate on single-stranded DNA, suggesting that DNA unwinding may proceed via a partially passive mechanism. However, the fastest dsDNA unwinding rates measured in the single-molecule unwinding assays approached the PcrA translocation speed measured on ssDNA