Chiral Bioanalysis of Warfarin Using Microbore LC with Peak Compression

A simplified packing and operating procedure for capillary electrochromatography (CEC) has been developed which draws from some of the more accessible features of published practice. The key elements of this methodology are use of an end-fitting and frit from a 1-mm microbore column in the initial phase of the packing, use of a conventional constant-flow HPLC pump for capillary packing, conditioning of the packed capillary using a conventional constant-flow HPLC pump with flow splitting, bedding down of the packed capillary under an electric field while fitted into the CE instrument and operation without pressurisation and without an end frit. Working in such a manner, CEC was carried out on a commercial test mixture and mixtures of steroids

Enantioselective separation of enantiomeric amides on three amylose-based chiral stationary phases: Effects of backbone and carbamate side chain chiralities Authors

A series of enantiomeric amides have been chromatographed on three amylose-based chiral stationary phases (CSPs): amylose tris(3,5-dimethylphenylcarbamate) (AD-CSP), amylose tris (S-phenylethylcarbamate) (AS-CSP), and amylose tris(R-phenylethyl-carbamate) (AR-CSP). The relative retentions and enantioselectives of the solutes on the three CSPs were compared and basic structure-retention relationships developed to describe the chromatographic results. The data indicate that for these solutes the observed elution order was a function of the chirality of the amylose backbone, while the magnitude of the enantioselective separations was affected by the chirality of the carbamate side chain. Chirality 9:173–177, 1997. © 1997 Wiley-Liss, Inc