216 research outputs found
Ligation and synthesis of chromatin deoxyribonucleic acid in vitro in neuronal, glial and liver nuclei isolated from adult guinea pig
A Research on the Treatment of Complex Sulphide Ores. III : Selective Sulphatization Process
With the aim of recovering zinc, copper. lead, iron and sulphur from complex sulphide ores, the selective-sulphatization flotation process has been proposed by the Institute. Complex sulphide ores are treated with , bulk flotation. The concentrate, after being reground, is subjected to the treatment of selective sulphatization. The object of the treatment lies in sulphatizing only the zinc and lead sulphides as far as possible, with minimum sulphatization of pyrite and chalcopyrite. In the treatment, the bulk concentrate is mixed with comparatively concentrated sulphuric acid, the mixture being kept at a temperature of around 150℃ for one hour. The sulphatization product is leached out with water, and the resultant zinc sulphate solution can be electrolysed for zinc. On the other hand, chalcopyrite, pyrite, and lead sulphate can be recovered from the leach residue by application of differential flotation. In the present report, the research on the selective sulphatization and the electrolytic recovery of zinc is given. First, to find out basic data for the treatment, a variety of sulphide minerals were subjected to the action of sulphuric acid of high concentration and the degree of sulphatization was examined. From the results, it was found that zinc minerals, including zinc blende and marmatite, galena, and pyrrhotite were readily sulphatized, while chalcopyrite and pyrite were almost unaffected. Second, the flotation concentrates from Hanaoka Mine, having the chemical composition as shown in Tables 2 and 4, were treated with the selective sulphatization. The representative result gave 97% of sulphatization percentage for zinc, while 3.6% and 2.0% for iron and copper, respectively. Optimum conditions for the tests were as follows : Concentration of H_2SO_4, 60 vol%; solid-liquid ratio, 1:1 ; treating time, 1 hr and treating temperature, 150~160℃. The solution leached out from the sulphatization product was subjected to tests for production of zinc under the electrolysis process in usual practice and the cathode zinc of 99.97% purity, containing 0.024% of lead, 0.0046% of copper and 0.0029% cadmium, was obtained. Finally, similar tests were repeated on a larger scale and the good results were also obtained
Menaquinone (vitamin K2) therapy for bronchial asthma. I. Mechanism of action menaquinone on allergic reactions.
The mechanism of action of the drug was investigated from various points of view. The findings may be summarized as follows: 1. In the experiments of the degranulation of mesenteric mast cells of rats, menaquinone proved to significantly inhibit the degranulation either in active or passive sensitization with the reagin-like antibody. 2. Menaquinone did not inhibit the formation of the reagin-like antibody. 3. In the experiements of the degranulation of basophilic granulocytes from patients of bronchial asthma, the rate of appearance of A form basophilic cells upon addition of the antihuman IgE goat serum was not markedly but significantly inhibited in the patients treated with menaquinone for long periods, as compared with that in the control, whereas the in vitro addition of menaquinone did not exert a significant inhibitory action.</p
Effects of Transmutation Products on the Formation of Interstitial Loops in High Purity Aluminum
Effects of the transmutation products during neutron irradiation in high purity aluminum on the formation of the interstitial loops were examined by the subsequent electron irradiation and electron microscopic observation. It is shown that Si atoms formed by (n, γ) nuclear reaction enhance the nucleation of interstitial loops. It is pointed out that p or α formed by (n, p) or (n, α) nuclear reactions form its complex with vacancies at high temperature and these complexes also enhance the nucleation of the interstitial loops
Production of anti-Candida antibodies in mice with gut colonization of Candida albicans.
BACKGROUND: Production of antibodies that are specific for allergens is an important pathological process in inflammatory allergic diseases. These contain the antibodies against antigens of Candida albicans, one of the normal microbial flora in an intestinal tract. We studied the effects of the prednisolone administration on the production of anti-Candida antibodies in the gastrointestinally C. albicans-colonized mice. METHODS AND MATERIALS: BALB/c mice, treated with antibacterial antibiotics to decontaminate indigenous intestinal bacterial flora, were inoculated intragastrically with C. albicans. The mice, in which C. albicans grows intestinally, were administered prednisolone to induce temporary immunosuppression. The Candida growth in their intestinal tract and their antibody response to Candida were examined. RESULTS: Antibiotic treatment allowed establishment of C. albicans gastrointestinal colonization, but did not cause subsequent systemic dissemination of C. albicans in all the animals. When these animals received an additional treatment with prednisolone, they showed a significantly higher population of C. albicans in their feces than those of animals treated with antibiotics alone, and the organisms were recovered even from their kidney. This systemic dissemination by C. albicans appeared to be temporal, because all the mice survived without any symptoms for more than 2 months. Examination of the serum titers of total immunoglobulin (Ig)E antibodies and specific IgE and IgG antibodies against Candida antigens demonstrated that titers of total IgE increased, partially by day 14 and clearly at day 27, in prednisolone-treated Candida-colonized mice. Without prednisolone treatment, an increment of the serum titer was scarcely observed. By day 27, corresponding to the increase of total IgE, the anti-Candida IgE and IgG titer increased in mice of the prednisolone-treated group. CONCLUSION: Administration of prednisolone to Candida-colonized mice can induce production of the IgG, IgE antibodies against Candida antigens, perhaps through temporal systemic dissemination of Candida from the intestinal tract
Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non-stimulated ovaries of patients with endometrial adenocarcinoma
PurposeImmature human oocytes from resected ovaries can be used for research and fertility preservation, though it is unknown whether it is feasible to transport oocytes for these purposes. This study examined in vitro maturation (IVM) outcomes after the transportation of human follicular fluid (HFF) containing oocytes. MethodsFourteen patients with endometrial adenocarcinoma were enrolled. Oocytes obtained from the resected ovaries of seven patients were transported with HFF by railway (transportation group). Samples of HFF from the other seven patients were not transported, and IVM was performed promptly (non-transportation group). The results of oocyte retrieval and IVM were compared. ResultsThe average ages in the transportation and non-transportation groups were 40.12.0 and 39.6 +/- 1.8years, respectively, and the average numbers of collected oocytes were 8.1 +/- 8.4 and 5.1 +/- 5.1, respectively. There was a significant negative correlation between the number of collected oocytes and age. The proportions of oocytes that reached meiosis II (maturation rate) after IVM were 38.6% and 69.2% in the transportation and non-transportation groups, respectively (P=0.013). ConclusionIn this preliminary study, the usefulness of the transportation of HFF was limited. Further studies on maintaining oocyte normality during transportation are necessary for becoming the effective method for research and clinical use
- …