Researching peers and disaster vulnerable communities: an insider perspective

Conducting research among peers and communities that a researcher also serves may be both daunting and rewarding. Researching peers may make the researcher feel uncomfortable raising certain questions that are sensitive or that could be construed to be testing their competencies. This paper is inclined more towards showing that it is advantageous to be an insider, whose position can facilitate collection of information that could not have been accessed, or revealed to an outsider. The paper reports on fieldwork conducted in a low-income country in Sub-Sahara Africa as part of a doctoral study with communities affected by disasters and those that work with such communities. The paper demonstrates the complexities of conducting such research and provides some insights that may be useful to insiders, outsiders or “in-betweeners” embarking on fieldwork in low-income countries and among vulnerable population struggling with manifold stresses and shocks

Ženy v top managemente: Prípadová štúdia spoločnosti EY

The thesis analyses the course and tempo of women´s careers in comparison with those of men, based on an analysis of my own research conducted at the company EY. The results of the research demonstrates, whether it is more challenging for women or men to achieve a managerial position. The thesis identifies and analyse the different factors that may influence the career of women, based on the theoretical findings and on a qualitative interview

Additional file 8: Figure S2. of Corepressive function of nuclear receptor coactivator 2 in androgen receptor of prostate cancer cells treated with antiandrogen

Full membranes of Western blotting (corresponding to Fig. 7). The membrane in the left panel showed NCOA2 staining part, and the right showed actin beta staining part. Starting from the left lane, NCOA2 expressions in LNCaP with bicalutamide and without bicalutamide, and positive control were shown. Twenty-seven ug of Jurkat cell lysate (Catalog #611451, BD Biosciences, Franklin Lakes, NJ, USA) was used as positive control. Densitometry data of Western blotting were attached below them. (DOC 1266 kb

Additional file 1: Figure S1. of Corepressive function of nuclear receptor coactivator 2 in androgen receptor of prostate cancer cells treated with antiandrogen

Screening of the siRNA efficacy on target mRNA expression levels. Catalog numbers were s20580, s20581, and s20582 (Neon Transfection System; Life Technologies, Carlsbad, CA, USA) for siRNA NCOA2 #1 (siNCOA2 #1), #2 (siNCOA2 #2), and #3 (siNCOA2 #3), respectively. Based on this result, siRNA NCOA2 #2 was selected, and the relevant data were indicated in Fig. 5. (DOC 54 kb

Additional file 5: Table S4. of Corepressive function of nuclear receptor coactivator 2 in androgen receptor of prostate cancer cells treated with antiandrogen

Ct values of quantitative PCR in LNCaP cells cultured with dihydrotestosterone-added media. (DOC 31 kb

Additional file 1: Figure S1. of A diagnostic marker for superficial urothelial bladder carcinoma: lack of nuclear ATBF1 (ZFHX3) by immunohistochemistry suggests malignant progression

Specificity and sensitivity of the seven anti-ATBF1 antibodies. Western blot analysis of ATBF1 in HEK293T cells using the seven anti-ATBF1 antibodies (Fig. 5a MB33, MB34, MB39, D1-120, MB44, MB47 and MB49). Lanes 1, 3, 5, 7, 9, 11, and 13 represent HEK293T cells with an HA-tag expression vector (pCI-HA). Lanes 2, 4, 6, 8, 10, 12, and 14 represent HEK293T cells containing an HA-tagged ATBF1 expression vector (pCI-HA-ATBF1). HEK293T cells were grown in DMEM supplemented with 10 % fetal bovine serum at 37 °C and 5 % CO2. HEK293T cells were transfected with the HA-tagged expression vector or the HA-tagged ATBF1 expression vector (HA-ATBF1) using transIT-293 reagent. (PPTX 215 kb