On the question of architecture and identity, in post-apartheid South Africa

Considerations as to personal and group identity seem to be everywhere these days, in the national news, in the latest pop/rap video and with respect to the very clothes that we choose to wear. Significantly, in post-apartheid South Africa, topical questions of ‘identity’ are driven from the racial politics of our past, and are mobilised toward our need to symbolise a new inclusive space of belonging. This paper looks at ways of representing and re-imagining collective identities through architectural design – that is design as process and product alike. The paper opens by introducing our political and social context before moving to consider more theoretical questions of subjectivity and of collective identity. Where after, the paper considers how architecture might contribute to questions of identity via three related lines of enquiry: 1) democratic judgement and creative process; 2) public space, both political and architectural; 3) and lastly, tectonic materiality and cultural memory. In so doing, the paper develops its discussion in dialogue with the work – amongst others – of Hannah Arendt, Paul Ricoeur and Couze Venn.Dit wil voorkom of persoonlike en groepsidentiteit deesdae oral is: in die nasionale nuus, in the nuuste pop/rap videos en in terme van die klere wat ons kies om te dra. Baie belangrik is die aktuele vraag oor ‘identiteit’, wat aangepor word deur die radikale politiek van ons verlede en ingespan word om ons behoefte om ‘n nuwe ruimtelikheid van behoort, te simboliseer. Hierdie artikel kyk na wyses waarop kollektiewe identiteite uitgebeeld en heruitgebeel work deur middel van ontwerp in argitektuur – dit is, terselfdertyd ontwerp as proses en produk. Die artikel spring weg met ‘n inleiding oor ons politieke en sosiale konteks voordat die fokus verskuif word na teoretiese vrae oor subjektiwiteit van die gemeenskaplike. Daarna beskou die artikel hoe argitektuur moontlik ‘n bydrae lewer tot vraestukke oor identiteit deur na drie verwante rigtings van ondersoek te verwys: 1) demokratiese oordeel en die kreatiewe proses; 2) publieke ruimte, beide polities en argitektonies; 3) and laastens, tektoniese materialiteit en kulturele onthou. Deur dit te doen, ontwikkel die artikel ‘n bespreking in dialoog met onder meer die werk van Hannah Arendt, Paul Ricoeur en Couze Vennhttp://www.journals.co.za/ej/ejour_sajah.htm

Breakdown and microbial uptake of marine viruses and other lysis products

To understand the roles of marine viruses in marine microbial food webs, it is important to determine rates and mechanisms of virus degradation and subsequent uptake of degraded virus material and other cell lysis products by heterotrophic marine bacteria. We radiolabeled and concentrated viruses and viral lysis products from either pure cultures (3H) or natural communities (3H and 33P) and added them to seawater samples of differing trophic status from coastal (mesotrophic) and offshore (oligotrophic) California waters and French Mediterranean waters (oligotrophic). Rates of degradation were determined by the loss of high molecular weight radiolabel over time and the fate of the degraded material (microbial uptake or accumulation in low molecular weight pools) was followed by size fractionation and/or acid extraction. Preliminary experiments with 3H-labeled, single-stranded RNA phage MS2 and marine phage H11/1 demonstrated that MS2 degraded significantly faster in coastal Santa Monica Bay seawater (2.5 ± 0.6% h-1), than the marine phage, H11/1 (0.99 ± 0.1% h-1). For labeled virus material from natural populations, rates of degradation were slower in oligotrophic waters (ranges from 1.0 to 3.3% h-1) than in mesotrophic waters (ranges from 4.9 to 6.0% h-1), corresponding to turnover rates of 1 to 4 d for this material. Degradation rates of labeled virus material are likely underestimates, because during preparation, degradation and uptake are continually occurring, resulting in accumulation of the less reactive products. The proportion of radiolabeled material taken up by microbes was greatest in oligotrophic waters, especially in the phosphate-limited Villefranche Bay, France, where most of the 33PO4-labeled material was taken up in less than 7 h. In contrast, the majority of degraded 3H-labeled material was not accumulated into biomass, and in 3 of 4 samples, accumulation was hardly detectable. The results suggest that viruses and lysis products are labile and turn over relatively rapidly, but often may not be efficiently incorporated into bacterial biomass

Rapid virus production and removal as measured with fluorescently labeled viruses as tracers

Pelagic marine viruses have been shown to cause significant mortality of heterotrophic bacteria, cyanobacteria, and phytoplankton. It was previously demonstrated, in nearshore California waters, that viruses contributed to up to 50% of bacterial mortality, comparable to protists. However, in less productive waters, rates of virus production and removal and estimates of virus-mediated bacterial mortality have been difficult to determine. We have measured rates of virus production and removal, in nearshore and offshore California waters, by using fluorescently labeled viruses (FLV) as tracers. Our approach is mathematically similar to the isotope dilution technique, employed in the past to simultaneously measure the release and uptake of ammonia and amino acids. The results indicated overall virus removal rates in the dark ranging from 1.8 to 6.2% h-1 and production rates in the dark ranging from 1.9 to 6.1% h-1, corresponding to turnover times of virus populations of 1 to 2 days, even in oligotrophic offshore waters. Virus removal rates determined by the FLV tracer method were compared to rates of virus degradation, determined at the same locations by radiolabeling methods, and were similar even though the current FLV method is suitable for only dark incubations. Our results support previous findings that virus impacts on bacterial populations may be more important in some environments and less so in others. This new method can be used to determine rates of virus degradation, production, and turnover in eutrophic, mesotrophic, and oligotrophic waters and will provide important inputs for future investigations of microbial food webs

Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria

A new nucleic acid stain, SYBR Green I, can be used for the rapid and accurate determination of viral and bacterial abundances in diverse marine samples. We tested this stain with formalin-preserved samples of coastal water and also from depth profiles (to 800 m) from sites 19 and 190 km offshore, by filtering a few ml onto 0.02 μn pore-size filters and staining for 15 min. Comparison of bacterial counts to those made with acridine orange (AO) and virus counts with those made by transmission electron microscopy (TEM) showed very strong correlations. Bacterial counts with AO and SYBR Green I were indistinguishable and almost perfectly correlated (r2 = 0.99). Virus counts ranged widely, from 0.03 to 15 × 107 virus ml-1. Virus counts by SYBR Green I were on the average higher than those made by TEM, and a SYBR Green I versus TEM plot yielded a regression slope of 1.28. The correlation between the two was very high with an r2 value of 0.98. The precision of the SYBR Green I method was the same as that for TEM, with coefficients of variation of 2.9%. SYBR Green I stained viruses and bacteria are intensely stained and easy to distinguish from other particles with both older and newer generation epifluorescence microscopes. Detritus is generally not stained, unlike when the alternative dye YoPro I is used, so this approach may be suitable for sediments. SYBR Green I stained samples need no desalting or heating, can be fixed with formalin prior to filtration, the optimal staining time is 15 min (resulting in a total preparation time of less than 25 min), and counts can be easily performed at sea immediately after sampling. This method may facilitate incorporation of viral research into most aquatic microbiology laboratories

Virus decay and its causes in coastal waters

Recent evidence suggests that viruses play an influential role within the marine microbial food web. To understand this role, it is important to determine rates and mechanisms of virus removal and degradation. We used plaque assays to examine the decay of infectivity in lab-grown viruses seeded into natural seawater. The rates of loss of infectivity of native viruses from Santa Monica Bay and of nonnative viruses from the North Sea in the coastal seawater of Santa Monica Bay were determined. Viruses were seeded into fresh seawater that had been pretreated in various ways: filtration with a 0.2-μm-pore-size filter to remove organisms, heat to denature enzymes, and dissolved organic matter enrichment to reconstitute enzyme activity. Seawater samples were then incubated in full sunlight, in the dark, or under glass to allow partitioning of causative agents of virus decay. Solar radiation always resulted in increased rates of loss of virus infectivity. Virus isolates which are native to Santa Monica Bay consistently degraded more slowly in full sunlight in untreated seawater (decay ranged from 4.1 in 7.2% h-1) than nonnative marine bacteriophages which were isolated from the North Sea (decay ranged from 6.6 to 11.1% h-1). All phages demonstrated susceptibility to degradation by heat-labile substances, as heat treatment reduced the decay rates to about 0.5 to 2.0% h-1 in the dark. Filtration reduced decay rates by various amounts, averaging 20%. Heat-labile, high- molecular-weight dissolved material (>30 kDa, probably enzymes) appeared responsible for about 1/5 of the maximal decay. Solar radiation was responsible for about 1/3 to 2/3 of the maximal decay of nonnative viruses and about 1/4 to 1/3 of that of the native viruses, suggesting evolutionary adaptation to local light levels. Our results suggest that sunlight is an important contributing factor to virus decay but also point to the significance of particles and dissolved substances in seawater

Effects of viral enrichment on the mortality and growth of heterotrophic bacterioplankton

The direct effects of viral enrichments upon natural populations of marine viruses and bacteria were studied in seawater from Santa Monica Bay, CA, USA. Active virus concentrates, or control additions (ultrafiltered seawater or autoclaved virus concentrate) were added to 2 1 incubations of protist-free seawater, and the effects were monitored for about 3 d. At the beginning of the experiments, the virus numbers reflected the expected addition of intact virus particles as determined by transmission electron microscopy (TEM). Subsequently, the mean frequency of visibly infected bacteria (FVIB; % bacteria which were visibly infected with 5 or more virus-like particles) was greater in the enriched incubations than in the controls. In controls, the estimated percent of bacteria that were infected remained constant at about 5 to 10 % of the total bacterial population, but with active enrichment, 10 to 35 % of the total bacterial population was infected at a given time. Therefore, by increasing the concentration of active viruses in seawater incubations we were able to increase the amount of bacterial mortality attributed to virus infection. Even with the presumed increase in bacterial mortality, the net increases in bacterial abundance in the samples that were enriched with active virus concentrate were higher than those seen in the controls. The vital abundance in bottles that were enriched with the active virus concentrate was significantly higher than that in the controls in Expts 2 and 3 (p < 0.05), but by the end of the experiments, viral abundances in the enriched incubations approached control levels. In Expts 1 and 2, rates of DOP hydrolysis were higher in the samples enriched with the active virus concentrate, and may have been due to an increase in the incidence of viral lysis. However, overall analysis of DCAA, DFAA, and DOP hydrolysis were quite variable and difficult to interpret. Results indicate that viral enrichment increased the incidence of bacterial infection and consequently stimulated the growth of subpopulations of non-infected heterotrophic bacterioplankton

Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I

Viruses are the most abundant biological entities in aquatic environments, typically exceeding the abundance of bacteria by an order of magnitude. The reliable enumeration of virus-like particles in marine microbiological investigations is a key measurement parameter. Although the size of typical marine viruses (20-200 nm) is too small to permit the resolution of details by light microscopy, such viruses can be visualized by epifluorescence microscopy if stained brightly. This can be achieved using the sensitive DNA dye SYBR Green I (Molecular Probes-Invitrogen). The method relies on simple vacuum filtration to capture viruses on a 0.02-μm aluminum oxide filter, and subsequent staining and mounting to prepare slides. Virus-like particles are brightly stained and easily observed for enumeration, and prokaryotic cells can easily be counted on the same slides. The protocol provides an inexpensive, rapid (30 min) and reliable technique for obtaining counts of viruses and prokaryotes simultaneously

Electroweak Precision Constraints on the Littlest Higgs Model with T Parity

We compute the leading corrections to the properties of W and Z bosons induced at the one-loop level in the SU(5)/SO(5) Littlest Higgs model with T parity, and perform a global fit to precision electroweak data to determine the constraints on the model parameters. We find that a large part of the model parameter space is consistent with data. Values of the symmetry breaking scale as low as 500 GeV are allowed, indicating that no significant fine tuning in the Higgs potential is required. We identify a region within the allowed parameter space in which the lightest T-odd particle, the partner of the hypercharge gauge boson, has the correct relic abundance to play the role of dark matter. In addition, we find that a consistent fit to data can be obtained for large values of the Higgs mass, up to 800 GeV, due to the possibility of a partial cancellation between the contributions to the T parameter from Higgs loops and new physics.Comment: 23 pages, 9 figures. Minor correction

Quantification of pathogens and markers of fecal contamination during storm events along popular surfing beaches in San Diego, California

Along southern California beaches, the concentrations of fecal indicator bacteria (FIB) used to quantify the potential presence of fecal contamination in coastal recreational waters have been previously documented to be higher during wet weather conditions (typically winter or spring) than those observed during summer dry weather conditions. FIB are used for management of recreational waters because measurement of the bacterial and viral pathogens that are the potential causes of illness in beachgoers exposed to stormwater can be expensive, time-consuming, and technically difficult. Here, we use droplet digital Polymerase Chain Reaction (digital PCR) and digital reverse transcriptase PCR (digital RT-PCR) assays for direct quantification of pathogenic viruses, pathogenic bacteria, and source-specific markers of fecal contamination in the stormwater discharges. We applied these assays across multiple storm events from two different watersheds that discharge to popular surfing beaches in San Diego, CA. Stormwater discharges had higher FIB concentrations as compared to proximal beaches, often by ten-fold or more during wet weather. Multiple lines of evidence indicated that the stormwater discharges contained human fecal contamination, despite the presence of separate storm sewer and sanitary sewer systems in both watersheds. Human fecal source markers (up to 100% of samples, 20-12440 HF183 copies per 100 ml) and human norovirus (up to 96% of samples, 25-495 NoV copies per 100 ml) were routinely detected in stormwater discharge samples. Potential bacterial pathogens were also detected and quantified: Campylobacter spp. (up to 100% of samples, 16-504 gene copies per 100 ml) and Salmonella (up to 25% of samples, 6-86 gene copies per 100 ml). Other viral human pathogens were also measured, but occurred at generally lower concentrations: adenovirus (detected in up to 22% of samples, 14-41 AdV copies per 100 ml); no enterovirus was detected in any stormwater discharge sample. Higher concentrations of avian source markers were noted in the stormwater discharge located immediately downstream of a large bird sanctuary along with increased Campylobacter concentrations and notably different Campylobacter species composition than the watershed that had no bird sanctuary. This study is one of the few to directly measure an array of important bacterial and viral pathogens in stormwater discharges to recreational beaches, and provides context for stormwater-based management of beaches during high risk wet-weather periods. Furthermore, the combination of culture-based and digital PCR-derived data is demonstrated to be valuable for assessing hydrographic relationships, considering delivery mechanisms, and providing foundational exposure information for risk assessment

On the complexity of Generalized Chromatic Polynomials

J. Makowsky and B. Zilber (2004) showed that many variations of graph colorings, called CP-colorings in the sequel, give rise to graph polynomials. This is true in particular for harmonious colorings, convex colorings, mcct-colorings, and rainbow colorings, and many more. N. Linial (1986) showed that the chromatic polynomial �(G;X) is #P-hard to evaluate for all but three values X = 0, 1, 2, where evaluation is in P. This dichotomy includes evaluation at real or complex values, and has the further property that the set of points for which evaluation is in P is finite. We investigate how the complexity of evaluating univariate graph polynomials that arise from CPcolorings varies for different evaluation points. We show that for some CP-colorings (harmonious, convex) the complexity of evaluation follows a similar pattern to the chromatic polynomial. However, in other cases (proper edge colorings, mcct-colorings, H-free colorings) we could only obtain a dichotomy for evaluations at non-negative integer points. We also discuss some CP-colorings where we only have very partial results