Diorganotin(IV) complexes with 2-furancarboxylic acid hydrazone derivative of benzoylacetone : synthesis, X-ray structure, antibacterial activity, DNA cleavage and molecular docking

Two new diorganotin(IV) complexes, Me2SnL and Ph2SnL, have been synthesized from the reaction of Me2SnCl2 and Ph2SnCl2 with the hydrazone H2L [H2L \ubc (Furan-2-yl) (5-hydroxy-3-methyl-5-phenyl-4,5- dihydro-1H-pyrazol-1-yl)-methanone] derived from furan-2-carbohydrazide and benzoylacetone. The new compounds have been characterized by elemental and spectroscopic analyses. The crystal structures of the monohydrate form of the ligand and of the Me2SnL derivative have been also determined by X-ray crystallography. Experimental evidences confirm the existence of the hydrazone ligand exclusively in cyclic form in both solution and solid state. On coordination to tin the hydrazone undergoes a ring opening reaction and a doubly deprotonation to act as a tridentate ligand via imine nitrogen and enolic oxygens. The tin atom in the complexes is five coordinate with geometry between square-pyramidal and trigonal-bipyramidal. The in vitro antibacterial activity of ligand and its complexes has been evaluated against Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The interaction between compounds with bacterial DNA was also studied by molecular docking. Our findings indicate that diphenyltin(IV) complex, by binding to DNA via minor groove to TATA sequence in genes upstream, has good activities along with the standard antibacterial drugs. Our agarose-gel electrophoresis experiments show that the ligand exert DNA cleavage, while Me2SnL and Ph2SnL did not

The beneficial effect of (-)-epigallocatechin-3-gallate in an experimental model of Alzheimer's disease in rat: A behavioral analysis

Background: Progressive cognitive decline is one of the hallmark symptoms of Alzheimer's disease (AD) which can be modeled by β-amyloid injection into specific regions of brain. Since epigallocatechin-3-gallate (EGCG) is a potent antioxidant agent which its role against oxidative stress and inflammation has been shown in prior studies, we tried to determine whether EGCG administration protects against β-amyloid-induced memory and coordination impairment in rats. Methods: Animals (male Wistar rats) were divided into four groups: sham operated, EGCG-pretreated sham operated (sham + EGCG), untreated lesion (lesion), and EGCG-pretreated lesion (lesion + EGCG). Animals in lesion, lesion + EGCG, and sham + EGCG groups received sterile saline or saline plus EGCG (10 mg/kg) intraperitoneally one day pre-surgery and every other day for three weeks. The lesion was induced one day after EGCG pretreatment by injection of 4 μl of sterile saline or water containing 2 nmol/μl β-amyloid (1-40) into the hippocampal fissure. For behavioral analysis, psychomotor coordination (PMC) index and spontaneous alternation behavior were assessed using Rota-rod Treadmill and Y-maze, respectively at the third week post-lesion. Results: We found that β-amyloid (1-40) injection into hippocampus can decrease these behavioral indexes in lesion group in comparison with sham group which is similar to behavioral changes in AD. On the other hand, pretreatment with EGCG can improve the PMC index and spatial Y-maze alternation in the lesion + EGCG group in comparison with lesion group. Conclusion: We concluded that EGCG can be effective in restoring β-amyloid-induced behavioral derangements in rats regarding coordination and memory abilities

Effect of chronic lithium administration on endothelium-dependent relaxation of rat corpus cavernosum: The role of nitric oxide and cyclooxygenase pathways

OBJECTIVE: To verify the effect of chronic lithium administration on the endothelium-dependent relaxation of rat corpus cavernosum, as lithium is a major drug for treating bipolar disorder and some studies showed that lithium might cause erectile dysfunction in such patients, by a mechanism as yet unknown. MATERIALS AND METHODS: LiCl (600 mg/L) was dissolved in drinking water and Sprague-Dawley rats received the solution for 30 days; control rats received tap water. After 30 days corporeal strips were prepared from both groups, mounted under tension in oxygenated organ baths, and pre-contracted with phenylephrine (7.5 μm). After equilibration, the strips were relaxed by acetylcholine (10 nm to 1 mm) in the presence or absence of indomethacin (a cyclooxygenase inhibitor; 20 μm). Furthermore, the relaxant responses to sodium nitroprusside (1 nm to 1 mm), a nitric oxide (NO) donor, were investigated in both groups. NADPH-diaphorase histochemistry was used to identify NO synthase within cavernosal tissue strips of both groups. RESULTS: The acetylcholine-dependent relaxation was significantly lower in lithium-treated rats than in controls. Although indomethacin decreased significantly the relaxant responses to acetylcholine in controls, it increased the relaxant responses in lithium-treated rats. NADPH-diaphorase staining was greater in the chronic lithium-treated than in control preparations. Sodium nitroprusside produced similar relaxation in both groups. CONCLUSION: Chronic lithium administration can impair the endothelium-dependent relaxation of rat corpus cavernosum; NO availability might decrease after lithium administration and the cyclooxygenase pathways might have a role in this effect. © 2006 The Authors

NADPH diaphorase staining of the corpus cavernosum in cholestatic rats: Role of nitrergic and opioid systems

Background: Relaxation of the corpus cavernosum plays an important role in penile erection. Previous studies have suggested that nitric oxide (NO) appears to be the most important relaxant involved in the erection process. The aim of the present study was to evaluate the effect of cholestasis in nNOS and eNOS activity of corpus cavernosum. Methods: forty-two adult male Sprague-Dawley rats were divided equally into seven groups: control, sham operated, 2-, 7-, and 14-day bile duct-ligated animals, 7-day bile duct-ligated chronically treated with L-NAME (3mg/kg/day, i.p.) and 7-day bile duct-ligated animals chronically treated with Naltrexone (20 mg/kg/day, i.p.). The animals in each group were killed and the cavernosal tissues analyzed histologically by light and transmission electron microscopy, with NOS activity detected on NANC nerves and endothelium using an NADPH-diaphorase staining technique. Results: our results showed that NADPH diaphorase staining in corporal NANC nerves and endothelium of sham-operated and control group had equal intensity. The staining was more intense in 2-day cholestatic rats than in control group, the staining intensity increased in 7-, and 14-day groups too. There were no significant differences between control group and 7-day cholestatic rats that had been treated chronically with L-NAME or Naltrexone. Conclusions: These results state that in corpus cavernosum of cholestatic rats there is a time-dependent increase in NOS activity of the corporal NANC nerves and endothelium. inhibition of nitric oxide and endogenous opioids by L-NAME or Naltrexone during cholestasis may play a key role in preventing the adverse effects of cholestasis. © 2008, Tehran University of Medical Sciences. All rights reserved

Transplantation of olfactory mucosa improve functional recovery and axonal regeneration following sciatic nerve repair in rats

Background: Olfactory ensheathing glia (OEG) has been shown to have a neuroprotective effect after being transplanted in rats with spinal cord injury. This study was conducted to determine the possible beneficial results of olfactory mucosa transplantation (OMT) which is a source of OEG on functional recovery and axonal regeneration after transection of the sciatic nerve. Methods: In this study, 36 adult female Sprague-Dawley rats were used. The sciatic nerve was transected in 24 rats and immediately repaired by sciatic-sciatic anastomosis, and randomly divided equally into two groups. The experimental group received the OMT at the transected site and the control group received the respiratory mucosa transplant. In another twelve rats as sham-operated animals, the sciatic nerve was exposed but no transection was made. DiI retrograde tracing was injected in the gastrocnemius muscle two months after surgery to allow visualization of the extent of axonal regeneration. Functional recovery was also assessed at 15, 30, 45 and 60 days after surgery using walking track analysis and sciatic function index (SFI) calculations. Results: The total number of DiI labeled motorneurones in the ventral horn (L4-L6) and the SFI scores were significantly higher in the group of rats that received olfactory mucosa rather than respiratory mucosa. Conclusions: The outcome indicates that olfactory mucosa is a useful treatment to improve nerve regeneration in mammals with peripheral nerve injury

Effect of chronic lithium administration on endothelium-dependent relaxation of rat mesenteric bed: Role of nitric oxide

The mechanism of action of lithium, an effective treatment for bipolar disease, is still unknown. In this study, the mesenteric vascular beds of control rats and rats that were chronically treated with lithium were prepared by the McGregor method, and the mesenteric vascular bed vasorelaxation responses were examined. NADPH-diaphorase histochemistry was used to determine the activity of NOS (nitric oxide synthase) in mesenteric vascular beds. We demonstrated that ACh-induced vasorelaxation increased in the mesenteric vascular bed of rats treated with lithium. Acute No-nitro-L-arginine methyl ester (L-NAME) administration in the medium blocked ACh-induced vasorelaxation in the control group more effectively than in lithium-treated rats, while the vasorelaxant response to sodium nitroprusside, a NO donor, was not different between lithium-treated and control groups. Acute aminoguanidine administration blocked ACh-induced vasorelaxation of lithium-treated rats, but had no effect in the control rats. Furthermore, NOS activity, determined by NADPH-diaphorase staining, was significantly greater in the mesenteric vascular beds from chronic lithium-treated rats than in those from control rats. These data suggest that the enhanced ACh-induced endothelium-derived vasorelaxation in rat mesenteric bed from chronic lithium-treated rats might be associated with increased NOS activity, likely via iNOS. Simultaneous acute L-NAME and indomethacin administration suggests the possible upregulation of EDHF (endothelium-derived hyperpolarizing factor) in lithium-treated rats. © 2007 NRC

An evaluation model for the implementation of hospital information system in public hospitals using multi-criteria-decision-making (MCDM) approaches

Background: Hospital Information System (HIS) is implemented to provide high-quality patient care. The aim of this study is to identify significant dimensional factors that influence the hospital decision in adopting the HIS. Methods: This study designs the initial integrated model by taking the three main dimensions in adopting HIS technology. Accordingly, DEMATEL was utilized to test the strength of interdependencies among the dimensions and variables. Then ANP approach is adapted to determining how the factors are weighted and prioritized by professionals and main users working in the Iranian public hospitals, in-volved with the HIS system. Results: The results indicated that "Perceived Technical Competence" is a key factor in the Human dimension. The respondents also believed that "Relative Advantage," "Compatibility" and "Security Concern" of Technology dimension should be further assessed in relation to other factors. With respect to Organization dimension, "Top Management Support" and "Vendor Support" are considered more important than others. Conclusion: Applying the TOE and HOT-fit models as the pillar of our developed model with significant findings add to the growing literature on the factors associated with the adoption of HIS and also shed some light for managers of public hospitals in Iran to success-fully adopt the HIS

An evaluation model for the implementation of hospital information system in public hospitals using multi-criteria-decision-making (MCDM) approaches

Background: Hospital Information System (HIS) is implemented to provide high-quality patient care. The aim of this study is to identify significant dimensional factors that influence the hospital decision in adopting the HIS. Methods: This study designs the initial integrated model by taking the three main dimensions in adopting HIS technology. Accordingly, DEMATEL was utilized to test the strength of interdependencies among the dimensions and variables. Then ANP approach is adapted to determining how the factors are weighted and prioritized by professionals and main users working in the Iranian public hospitals, in-volved with the HIS system. Results: The results indicated that "Perceived Technical Competence" is a key factor in the Human dimension. The respondents also believed that "Relative Advantage," "Compatibility" and "Security Concern" of Technology dimension should be further assessed in relation to other factors. With respect to Organization dimension, "Top Management Support" and "Vendor Support" are considered more important than others. Conclusion: Applying the TOE and HOT-fit models as the pillar of our developed model with significant findings add to the growing literature on the factors associated with the adoption of HIS and also shed some light for managers of public hospitals in Iran to success-fully adopt the HIS. © 2018 Ali Aliakbar Esfahani et al

An evaluation model for the implementation of hospital information system in public hospitals using multi-criteria-decision-making (MCDM) approaches

Background: Hospital Information System (HIS) is implemented to provide high-quality patient care. The aim of this study is to identify significant dimensional factors that influence the hospital decision in adopting the HIS. Methods: This study designs the initial integrated model by taking the three main dimensions in adopting HIS technology. Accordingly, DEMATEL was utilized to test the strength of interdependencies among the dimensions and variables. Then ANP approach is adapted to determining how the factors are weighted and prioritized by professionals and main users working in the Iranian public hospitals, in-volved with the HIS system. Results: The results indicated that "Perceived Technical Competence" is a key factor in the Human dimension. The respondents also believed that "Relative Advantage," "Compatibility" and "Security Concern" of Technology dimension should be further assessed in relation to other factors. With respect to Organization dimension, "Top Management Support" and "Vendor Support" are considered more important than others. Conclusion: Applying the TOE and HOT-fit models as the pillar of our developed model with significant findings add to the growing literature on the factors associated with the adoption of HIS and also shed some light for managers of public hospitals in Iran to success-fully adopt the HIS. © 2018 Ali Aliakbar Esfahani et al