Lithiase urinaire (analyse des causes et orientation thérapeutique avec conseils aux patients à l'officine)

Les lithiases sont de plus en plus fréquentes. Leurs compositions retranscrivent l'évolution des populations, le développement des pays et l'environnement des individus. l'alimentation peut permettre de prévenir les récidives. Le pharmacien doit participer à l'éducation thérapeutique du patient en prodiguant des conseils hygiéno-diététiques. Cela fait partie de son rôle d'acteur de santé publique. Plusieurs médicaments constituent un facteur de risque car ils peuvent induire la formation de calculs et/ou se retrouver dans leurs compositions. Les acteurs médicaux doivent travailler ensemble afin d'évaluer ce risque et mettre si besoin en place une stratégie pour prévenir l'appartion de lithiases chez les patients concernés.CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocSudocFranceF

Projet pédagogique d'illustration d'un cours de physiologie-pharmacologie appliqué à l'électrophysiologie cardiaque

Cette thèse se présente sous la fomle atypique d'un CD-ROM. Il s'agit d'un travail expérimental d'illustration d'un cours de physiologie-pharmacologie appliqué à l'électrophysiologie cardiaque. Ce document destiné aux étudiants est illustré et se veut interactif, d'où son support numérique. Le texte ci-après en retrace l'origine, en indique les objectifs et en précise le mode d'utilisation.CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocSudocFranceF

Influence of endothelial cells on structure, biochemistry and functionality of epidermis reconstructed on synthetic porous membrane

The model of keratinocytes cultured on a synthetic porous membrane at the air-liquid interface leads to the formation of a pluristratified and cornified epidermis with histological and biochemical characteristics near those observed in vivo. In the present study, we evaluated the effect of proliferative endothelial cells on epidermalization. Keratinocytes were grown in three culture conditions: in defined medium (DM; control), in medium previously conditioned by proliferative endothelial cells (CM) and in medium with proliferative endothelial cells (pEC). The structures of reconstructed epidermis were analyzed by electron microscopy, their biochemistry by DNA, protein and cytokine analyses and finally their functionality was evaluated by estradiol and water absorption testing. Ultrastructural analysis showed a well-developed and cornified epidermis for each culture condition. In addition, living epidermis was thinner in the presence of endothelial cells, revealing faster epidermal differentiation. DNA and protein analyses were in accordance with these results. Secreted soluble factors varied according to culture conditions. At 37°C, the permeability of reconstructed epidermis in DM, in CM or with pEC was 5- to 10-fold higher than that of native human epidermis with both tracers. Laminin coating of the inserts led to similar absorption results except for the DM where the barrier function to estradiol was decreased 2-fold. At 32°C, reconstructed and native epidermis were, respectively, 1.5- and 2-fold less permeable to estradiol compared to 37°C. In conclusion, this model is adequate for fundamental and pharmacological studies since it allows the study of interactions between two cell types without their direct contact as well as percutaneous absorption tests directly performed in the modified culture chamber