Fetal Triploidy Syndrome: a case report from Global Reference Laboratory, Mumbai, India

Triploidy is a complete extra set of chromosomes. In the current case report, we present the case of a 29-year-old pregnant female who was referred to Department of Genetics, Metropolis Healthcare Ltd, Mumbai. Real-time sonography of the gravid uterus was done using a 3C RS multi-frequency probe. Placenta was observed to be posterior. The findings were suggestive of a single viable foetus with an average gestational age of 14.6 weeks at 14-15 weeks of pregnancy and only mild placental thickening at 17-18 weeks of pregnancy was detected on Ultrasound at the time of Amniocentesis. The fetal chromosomal study on amniotic fluid by Fluoroscence insitu hybridization (FISH) revealed trisomy status for chromosome 13, 18, 21, X and Y in 100% of the cells analysed and the fetal karyotype revealed a presence of extra set of chromosomes (69) in all the analysed cells. Parental blood karyotype was done for checking cytogenetic abnormality or variations. FISH studies with POC specimen revealed Trisomy status for chromosomes 13, 18, 21 and presence of extra Sex chromosome (XXY) in all cells analysed

Parametric optimization of wear parameters of hybrid composites (LM6/B4C/fly ash) using Taguchi technique

Wear is prominent in sliding components, so tribology property plays a major role in automotive as well as in the aerospace industries. In this work, Aluminium alloy LM6/B4C/Fly Ash hybrid composites with three different weight percentages of reinforcement were fabricated using the low-cost stir casting technique, and the experiments were conducted based on the Design of Experiments (DoE) approach and optimized using Taguchi’s Signal to noise ratio (S/N) analysis. The analysis was conducted with process parameters like Sliding Speed (S), Sliding distance (D), load (L) and reinforcement percentage (R %), the responses are Coefficient of Friction (COF) and Specific wear rate (SWR). Aluminum alloy reinforced with 9 wt% hybrid (LM6 + 4.5% B4C + 4.5% Fly Ash) has a low density and high hardness compared with other composites and base alloys. The optimum parameters for obtaining minimum SWR are S - 1 m/s, D - 500 m, L - 45 N, and R% - 6 wt% Hybrid (3% Fly ash and 3% boron carbide). The optimum parameters for obtaining minimum COF are S - 1.5 m/s, D - 500 m, L - 30 N, and R% −9 wt% Hybrid (4.5% Fly ash and 4.5% boron carbide). Load (28.34%) is the most significant parameter for obtaining minimum SWR, and DL (31.62%) for obtaining minimum COF. SEM images of the worn pins show the various wear mechanisms of the AMCs. The hybrid composite produced is new and these may be used for piston liner and brake pad applications

Nicotinamide-based agglomerates of ibuprofen: formulation, solid state characterization and evaluation of tableting performance with in-silico investigation

Abstract Background The objective of the present investigation was to obtain directly compressible agglomerates of ibuprofen with nicotinamide by a quasi-emulsification solvent diffusion technique. Ibuprofen-nicotinamide agglomerates were prepared by quasi-emulsification solvent diffusion technique using ethanol (good solvent), water (poor solvent), and chloroform (bridging liquid). The prepared agglomerates were characterized by ATR-FTIR, powder X-ray diffraction, differential scanning calorimetry, and scanning electron microscopy and were evaluated for tableting performance and in vitro drug release. To appropriately identify the hydrogen bonding sites, a thorough understanding of the structures of API and coformer is necessary, hence molecular docking approach was implemented to depict the interaction between the proposed coformer and COX-2 protein (PDB Id:4PH9). Results The percent yield of agglomerates was in the range of 85–98 w/w%, and drug content for all batches was in the range of 96–99%. The microphotographs showed irregular circularly shaped agglomerates. ATR-FTIR study showed a strong possibility of hydrogen bonding between ibuprofen and nicotinamide. The crystallinity of ibuprofen was slightly reduced and confirmed by P-XRD and DSC. Crushing strength and friability studies showed good handling qualities of ibuprofen agglomerates. Heckel plot studies showed low mean yield pressure and high tensile strength, indicating excellent compressibility and compactibility of ibuprofen agglomerates. More than 90% drug release was obtained within 60 min in PBS (pH 7.4). The docking studies revealed that nicotinamide individually has –CDOCKER energy 16.8109 where coformer showed 29.0584, which indicates coformer has a better binding affinity to target as compared to nicotinamide individual. Conclusions It can be concluded that the agglomerates improved the dissolution, tableting performance, and solid-state properties of ibuprofen and hence can be useful to improve the therapeutic performance of ibuprofen. Graphic Abstrac

Design and Structural Simulations of a Custom Li-Po Accumulator for Low Range, Lightweight, Single-Seater, Open Cockpit, and Open-Wheeled Racecar

Electric, hybrid, and fuel cell vehicles are the future of the automobile industry, and power source design is one of the most crucial steps in designing these vehicles. This paper aims to design and structurally simulate a custom accumulator—which powers an electric vehicle, for a lightweight, single-seater formula-style racecar. The work is dependent on the model-based design and CAD model approach. Mathematical modeling on SCILAB is used to model equations to get the characteristics of the accumulator, such as the energy, capacity, current, voltage, state of charge, and discharge rates. The output of this model gives the configuration of the battery pack as several cells in series and parallel to adequately power the tractive system. An accumulator container is designed to safeguard the cells from external impacts and vibrational loads, which otherwise can lead to safety hazards. Following this, the Finite Element Analysis (FEA) performed on the accumulator resulted in maximum peak deformation of 0.56 mm, ensuring the safety check against various external loads. Further, the finer stability of the battery pack was virtually validated after performing the vibrational analysis, resulting in a deformation of 3.5493 mm at a 1760.8 Hz frequency

Design and Structural Simulations of a Custom Li-Po Accumulator for Low Range, Lightweight, Single-Seater, Open Cockpit, and Open-Wheeled Racecar

Electric, hybrid, and fuel cell vehicles are the future of the automobile industry, and power source design is one of the most crucial steps in designing these vehicles. This paper aims to design and structurally simulate a custom accumulator—which powers an electric vehicle, for a lightweight, single-seater formula-style racecar. The work is dependent on the model-based design and CAD model approach. Mathematical modeling on SCILAB is used to model equations to get the characteristics of the accumulator, such as the energy, capacity, current, voltage, state of charge, and discharge rates. The output of this model gives the configuration of the battery pack as several cells in series and parallel to adequately power the tractive system. An accumulator container is designed to safeguard the cells from external impacts and vibrational loads, which otherwise can lead to safety hazards. Following this, the Finite Element Analysis (FEA) performed on the accumulator resulted in maximum peak deformation of 0.56 mm, ensuring the safety check against various external loads. Further, the finer stability of the battery pack was virtually validated after performing the vibrational analysis, resulting in a deformation of 3.5493 mm at a 1760.8 Hz frequency

Analysis of the aerodynamic characteristics of an ejection seat system using computational fluid dynamics

In the present work, an investigation of the aerodynamic characteristics of an ejection seat occupant is carried out using the commercially available computational fluid dynamics software ANSYS Fluent. 3D Reynolds-averaged Navier–Stokes equations are solved to obtain the aerodynamic coefficients of the ejection seat system. For this analysis, an unstructured grid is generated for the ejection seat occupant using ANSYS meshing. Validation is carried out and the performance of three different turbulence models is analyzed at Mach 0.6. Based on the most suitable turbulence model, further analysis of the aerodynamic coefficients of the ejection seat occupant is calculated at Mach numbers of 0.35, 0.45, 0.55, 0.65, and 0.75. For all values of Mach, the angle of attack is varied from −15° to 15° in 5° increments and the yaw angle is varied from 0° to 60° in 10° increments. Based on the results, it is observed that the magnitude of the axial force decreases with increasing angle of attack and yaw angle. Similarly, the normal force coefficient and pitching moment coefficient decrease with increasing angle of attack. Finally, the side force coefficient, yawing moment, and rolling moment coefficients increase with increasing yaw angle.Web of Science9art. no. 125505