Peroksidacija lipida i antioksidativni status u psa mješovite pasmine s kožnim metastazama prenosivoga veneričnoga tumora - prikaz slučaja.

A metastatic form of cutaneous transmissible venereal tumor was diagnosed in a mongrel dog. The dog had non-fluctuating and ulcerating neoplastic nodules throughout the subcutis with a history of spontaneous regression and recurrence of new growths at different sites. A presumptive diagnosis was made based on fine needle aspiration cytology of neoplastic nodules and an impression smear of ulcerated lesions, which was confirmed by histopathology. Erythrocyte oxidant-antioxidant status revealed increased lipid peroxidation and decreased antioxidant enzymes, which might have been due to the direct influence of the tumor or by associated factors such as tumor necrosis and/or the inflammatory changes. Increased early apoptosis was observed in peripheral blood leukocytes. Treatment with Vincristin along with other supportive therapy yielded an unfavorable outcome due to the advanced stage of malignancy.Metastaze kože prenosivoga veneričnoga tumora dijagnosticirane su u psa mješovite pasmine. Ustanovljeni su nefluktuirajući i ulcerativni neoplastični čvorovi u potkožju, koji su spontano nestajali, ali uz pojavu novih na različitim mjestima. Sumnja na pojavu tumora bila je postavljena na osnovi citološkoga nalaza aspiriranoga tkiva novotvorina, a potvrđena histopatološkom pretragom materijala uzetog iz ulceracijskih promjena. Antioksidacijski status eritrocita pokazao je povećanu peroksidaciju lipida i smanjenu aktivnost antioksidacijskih enzima što može biti zbog izravnoga utjecaja tumora ili zbog drugih čimbenika kao što je tumorska nekroza i/ili upalne promjene. Pojačana rana apoptoza zabilježena je u leukocitima periferne krvi. Primjena vinkristina uz potporno liječenje drugim lijekovima nije dala zadovoljavajuće rezultate zbog uznapredovaloga stupnja zloćudnosti

Uklanjanje obostranog dermoida na bulbarnoj spojnici šteneta pasmine labrador retriver elektrokauterom - prikaz slučaja.

A Labrador retriever pup with a bilateral dermoid in the eyes was treated successfully by the use of a unipolar hand held electrocautery unit.Štene pasmine labrador retriver s obostranim dermoidom u očima bilo je uspješno izliječeno upotrebom jednostranog ručnog elektrokautera

Not Available

Not AvailableEssential oils have applications in folk medicine, food preservation, and as feed additives. The essential oils of Lantana camara Linn. (Verbenaceae), Ageratum houstonianum Mill. (Asteraceae) and Eupatorium adenophorum Spreng. (Asteraceae) were analyzed by Gas chromatography - mass spectrometry (GCMS). In L. camara oil, of the total identified (83.91%) volatile constituents, five constituents [3,7,11 - trimethyl - 1,6,10 - dodecatriene (28.86%), β - caryophyllene (12.28%), zingiberene (7.63%), γ - curcumene (7.50%) and α - humulene (3.99%)] represented the major ones. In A. houstonianum oil, among the total identified volatile constituents (94.51%), three [precocene - II (52.64%), precocene - I (22.45%) and β - caryophyllene (9.66%)] represented the major ones. In E. adenophorum oil, of the total identified volatile constituents (84.95%), six [1 - napthalenol (17.50%), α - bisabolol (9.53%), bornyl acetate (8.98%), β - bisabolene (6.16%), germacrene - D (5.74%) and α - phellandrene (3.85%)] represented the major ones. The antibacterial activity expressed as Minimum Bactericidal Concentration (MBC) (μg/mL) was determined by the broth dilution method. The essential oil of E. adenophorum had antibacterial activity against Arthrobacter protophormiae, Escherichia coli, Micrococcus luteus, Rhodococcus rhodochrous, and Staphylococcus aureus with MBC values of 200, 100, 100, 12.5, and 200, respectively. The essential oil of A. houstonianum showed antibacterial activity against M. luteus and R. rhodochrous with MBC of 100 and 12.5, but not against A. protophormiae, E. coli, and S. aureus. The essential oil of L. camara showed antibacterial activity against A. protophormiae, M. luteus, R. rhodochrous and S. aureus with MBC of 50, 25, 12.5, and 200, respectively, but not against E. coli. MBC was lowest for R. rhodochrous for all the three essential oils.Not Availabl

Hepatotoxicity of Eupatorium adenophorum to rats

Freeze dried Eupatorium adenophorum leaf powder mixed in rat feed at a level of 25% elicited hepatotoxicity. The affected animals were jaundiced and had marked increase in plasma bilirubin levels and activities of alkaline phosphatase, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase. The liver of intoxicated animals had focal areas of necrosis and bile duct proliferation. Elevation in plasma bilirubin concomitant with alterations in enzyme profile and histopathological lesions are consistent with liver injury and cholestasis. This is the first report of the toxicity of E. adenophorum to rats

10 years journey of ICAR-NIASM

Not Available10 years journey of ICAR NIASM (2009-2019) in pictorial form is presented in this bulletin.Not Availabl

Not Available

Not AvailableEupatorium genus grows wild in many parts of the world. A number of species of Eupatorium are toxic to grazing animals. Milk sickness in humans is caused by ingestion of milk of the animals reared on the pastures infested with Eupatorium rugosum (white snakeroot). While some information is available on the toxins in various species of Eupatorium, ambiguities still persist in extrapolation of the data to field incidence of toxicosis. Eupatorium genus has been used for its medicinal properties for many decades. A number of bioactive natural products have been reported in the extracts of Eupatorium spp. and the genus is a promising bioresource for preparation of drugs and value‐added products.Not Availabl

Hepatotoxicity and Cholestasis in Rats Induced by the Sesquiterpene, 9-oxo-10,11-Dehydroageraphorone, Isolated from Eupatorium adenophorum

: Eupatorium adenophorum leaves cause hepatotoxicity and cholestasis in rats. The hepatotoxicant has been characterized as 9-oxo-10,11- dehydroageraphorone (ODA), a cadinene sesquiterpene. Oral administration of ODA, mixed in feed to rats, caused jaundice in 24 h. The liver of the intoxicated animals had focal areas of hepatocellular necrosis, proliferation, and dilation of bile ducts with degenerative changes in the lining epithelium. There was marked increase in the conjugated form of plasma bilirubin and in the activities of the enzymes glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, alkaline phosphatase, lactate dehydrogenase, g-glutamyltranspeptidase, glutamate dehydrogenase, and 50-nucleotidase. The histopathological lesions in liver and biochemical profile of marker enzymes show that ODA induced hepatotoxicity and cholestasis in rats. This is the first report on the toxicity of a cadinene sesquiterpene in rats.