Synthesis and Characterization of Nanodiamond-Doxorubicin (Dox) Conjugate for Effective Delivery against MCF-7 Cell Lines

In this work, we have introduced a carbon nanomaterial (nanodiamond), to bind with anticancer drug doxorubicin (DOX) with via amide bond conjugation for cancer drug delivery and therapy. Nanodiamond (ND) was initially carboxylated by the surface modification along the treatment with strong alkaline solution (H2SO4:HNO3) and then activated the carboxyl moiety of ND with the addition of EDC. Anticancer drugs were bound to the ND through a succession of chemical modifications by adipic acid dihydrazide (ADH). The ND-Drug conjugate was analyzed by Nuclear Magnetic Resonance (1H-NMR) Spectroscopy, Fourier Transform Infrared (FTIR) Spectroscopy and Mass Spectroscopy (MS), Atomic Force Microscopy (AFM), Particle size, Zeta potential, Drug release, SRB assay against MCF-7 cells, and DNA fragmentation. Spectroscopic analysis confirms the conjugation of nanodiamond with different anticancer drug. AFM photomicrograph represents the surface morphological features of ND-DOX conjugates. In- vitro investigation showed that ND-DOX conjugates have slow and sustained drug release characteristics. In-vitro cytotoxicity studies, an enormous cytotoxic potential of ND-Drug conjugates were showed against cancer cell line. Above all findings were suggested that the ND-DOX conjugates may be a potential inhibitor of MCF-7 cancer cells to act as a drug candidate. According to all these data it can be confirm that the ND-DOX conjugates could be an effective agent for drug delivery and could be promising in future for tumor targeting strategy. Keywords: Nanodiamond, Sustained Release, Drug Delivery, Cytotoxicity, Conjugate

Evaluation of Anti-Hyperlipidemic Activity of Ethanolic Extract of Cassia Fistula Leaf

The present study was designed to perform evaluate antihyperglycemic activity of ethanolic extract of Cassia Fistula leaf. Leaf of cassia fistula was extracted using ethanol as solvent by soxhlet apparatus. The evaluation of antihyperlipidemic activity was done using High Fat Diet induced hyperlipidemia models in Wistar albino rats. The work entitled evaluation of anti-obesity activity of leaf of Cassia fistula was to determine the efficacy and safety in experimental animals. Both aqueous and alcoholic extract of leaf of Cassia Fistula (Linn.) have shown significant reduction in weight of heart and both the kidney as compared to cafeteria diet fed animals confirming their anti-obesity property. The aqueous and alcoholic extract of leaf of Cassia Fistula (Linn.) has shown significant decrease in serum levels of Total-Cholesterol, LDL-Cholesterol, VLDL-Cholesterol and Triglyceride. Keywords: Antihyperglycemic, cassia fistula, hyperlipidemia, High fat diet, alcoholic extrac

Simultaneous determination of Nitazoxanide and Ofloxacin in pharmaceutical preparations using UV-spectrophotometric and high performance thin layer chromatography methods

Simple, precise, and accurate UV-Spectrophotometric and high-performance thin-layer chromatography (HPTLC) methods for the simultaneous determination of Nitazoxanide and Ofloxacin in pharmaceutical preparations have been developed and validated. The method was developed using aluminum plates pre-coated with silica gel 60 F254 HPTLC plates as a stationary phase with toluene:chloroform:carbon tetra chloride:toluene:glacial acetic acid solutions in the proportion of (10:5:3:0.5 v/v/v/v) as mobile phase. Densitometric quantification was performed at 241 nm. Well-resolved bands were obtained with RF values 0.36, 0.57 and 0.63 for Rosiglitazone maleate, Nitazoxanide, and Ofloxacin, respectively. Rosiglitazone maleate was used as an internal standard. The calibration curves were linear within the concentration range of 5–25 μg/ml for each drug. Two simple spectrophotometric methods have been developed for simultaneous estimation of Nitazoxanide, and Ofloxacin from tablet dosage form. The first method, simultaneous equation method, involves the measurement of absorbances at two wavelengths 221.8 nm (λmax of Nitazoxanide) and 244.3 nm (λmax of Ofloxacin), and the second method is First order derivative spectroscopy, wavelengths selected for quantitation were 263.6 nm for Nitazoxanide and 269.2 nm for Ofloxacin. The proposed method gave good validation results and the statistical analysis performed proved that the method is precise, accurate and reproducible, and hence can be employed for routine analysis of Nitazoxanide and Ofloxacin in bulk and commercial formulations