Probiotic Supplement Enhances the Hematological Parameters and Immune Response of Common Carp against Fish Pathogen Aeromonas hydrophila

Probiotic bacteria play an important role in the growth and health of aquatic animals by improving their immune system.  A 60-day experiment study was carried out to evaluate the effect of probiotic bacteria Bacillus subtilis dietary supplement enhances the hematological parameters and Immune response of fish Common carp against the pathogenic Aeromonas hydrophila.  The experimental fish with an average mean weight of 15.15 ± 1.05 g were distributed randomly into 3 groups (Control (CTRL (Basal feed and without A. hydrophila), Exp1 (Basal feed and with A. hydrophila, and Exp2 (108 CFU/ml with probiotic supplement with A. hydrophila). On the 56th day, experimental groups were then challenged with A. hydrophila (1.0 × 107 CFU/mL), after 72 hrs of infection, blood samples were collected randomly from five fish from each group.  Results showed that probiotic supplementation improved Hematological parameters such as RBCs, WBCs, Hb, PCV, and Immune response parameters (Nitroblue Tetrazolium Activity, aminotransferase, Serum lysozyme activity, total protein, and immunoglobulin). This present study suggests that the probiotic diet enhances the disease resistance against Aeromonas hydrophila in the culture of Common carp

Effect of Feeding Strategy of Probiotic Bacillus on Improving Water Quality and Growth Performance of Common carp (Cyprinus carpio) Fingerlings

Bacterial probiotics (Bacillus subtilis) and dietary supplements improve the growth and biochemical performance of Common carp (Cyprinus carpio) fingerlings. Fish were nourished for 60 days with a control diet and experimental diets containing B. subtilis 106 to 109 CFU/ ml-l respectively. Dietary supplementation of B.  subtilis feeding trial was conducted for 60 days to improve water quality and growth parameters were observed through weight gain, Specific growth rates, and Percent survival of C. carpio fingerlings all through the test period

Dietary Probiotic Supplementation on Hematological and Immunological Parameters of Indian Major Carp (Labeo rohita)

A 60-day feeding trial was conducted to evaluate the influence of dietary supplementation of probiotic bacteria (Lactobacillus acidophilus) to improve the hematological and immunological parameters of Indian Major carp Rohu (Labeo rohita).  The rohu Fingerlings (mean body weight 6.0±0.6g) were randomly distributed into Four treatment groups each having 10 fishes fed with basal diet and a probiotic supplement containing L. acidophilus (1.0×107 CFU/g), then challenged with A. hydrophila (0.1 × 107 CFU/mL) was injected intraperitoneally with 0.1ml concentration of 107 CFU/ml on 55th day. After 72 hours, the blood samples were collected from five fish from each group. The hematological parameters such as hemoglobin (Hb; g/dL), red blood cell (RBC; ×106/mm3), white blood cell (WBC; ×103/mm3), and Packed corpuscular volume (PCV) along with immunological parameters of total immunoglobulin, serum lysozyme activity, and Aminotransferases were examined and compared with the control group. The fish fed with probiotics showed significant improvement in hematological and Immunological Parameters (P≤0.05) as compared to control. This study proved that probiotic bacteria L. acidophilus are resistant to pathogenic A. hydrophila and can contribute to improving the immune system, survival rate, and growth of experimental fish against the fish pathogen A. hydrophila