ISOLATION AND CHARACTERIZATION OF SHATAVARIN IV FROM ROOT OF ASPARAGUS RACEMOSUS WILLD

Objective: To develop a method for the isolation of the steroidal saponin Shatavarin IV from the root of Asparagus racemosus Willd.Methods: Powder of Asparagus racemosus dried roots were defatted with hexane and extracted with methanol. The methanolic extract was used for isolation of pure compound by column chromatography. Isolated compound was identified by melting point analysis, IR and Mass spectroscopic analysis. Purity was checked by High-performance thin layer chromatography (HPTLC).Results: The highest yield of Shatavarin IV was obtained 401.1 mg from 250 g crude powder and highest purity was achieved 66% of lower atmospheric temperature (17-22 °C).Conclusion: Shatavarin IV isolation using column chromatography was affected with the change in atmospheric temperature. Maximum yield was obtained at the lower temperature i.e. 17-22 °C, whereas at higher temperature the Shatavarin IV showed degradation in purity and yield.Â