Determining drug resistance patterns of Clostridium perfringens isolates from acute necrotic enteritis (NE) outbreaks

In this study, after separating 40 Clostridium perfringens isolates from affected broiler flocks, the antibacterial susceptibility test revealed 39 drug resistance patterns in which 95% of the isolates were distributed into 38 different patterns (one isolate in each pattern) and only 5% of the isolates were placed in a single pattern (two isolates in a pattern). All 40 Clostridium perfringens isolates demonstrated resistance to chloramphenicol, vancomycin and sulfamethoxazole+trimethoprime, ranging from 0 to 17.5% while tetracycline, lincomycin and neomycin sulfate had a high level of resistance from 80 to 87.5%. Also an isolate demonstrated multiple resistance to more than 14 antibacterial compounds

Evaluation of serumic Uric acid,Total Protein, Albumin, Calcium and Phosphorus levels in laying hens afflicted by the gout syndrome

Gout syndrome is accounted for one of the prevalent causes of mortality in poultry farms. Gout is recognized by deposition of urates in kidneys, on serous surfaces of the heart, liver, mesenteries, air sacs and peritoneum. In some cases, these deposits may be observed on surfaces of muscles, synovial sheaths of tendons and joints, too. These deposits are like chalky coating and appear grossly. Gout could be seen in two forms, articular gout and visceral gout, and it is a disease with numerous causes.  The causing factors of the gout are classified in three groups which consist of nutrient and management factors, infectious factors, and toxic factors. For evaluation of biochemical parameters in gout disease, we surveyed and diagnosed in three farms of egg laying poultry breeding (HY-Line) affected to gout syndrome during spring and summer of 1386. In this study, from the above- mentioned poultry farms a number of 20 affected, and 20 healthy chickens (totally 120) were chosen and Blood samples of all animals were collected from wing veins and their sera were harvested by centrifuging and the levels of Uric acid, Total Protein, Albumin, Calcium and Phosphors parameters were measured by diagnostic laboratory kits. After taking blood, they were autopsied, and according to autopsy signs and syndromes, the diagnosis of gout was confirmed. Results of this study showed  significant increase on mean serumic Uric acid, Total Protein, Albumin, Calcium and Phosphorus in chickens affected by gout in comparison with the  healthy groups(

Serological survey of Ornithobacterium rhinotracheale infectious in broiler flocks of Ardabil province

Ornithobacterium rhinotracheale (ORT) is a gram negative, non-motile, and rod shaped bacterial pathogen causing respiratory tract disease in poultry and cause economic losses in poultry industry. The Objective of this study, was investigation of serological status of Ardabil province broiler flocks in view of ORT infection. For this purpose from 25 broiler flocks, 450 serum samples were taken in last week of age, and then investigated with IDEXX ELISA kit. Results showed that 72.2 percent (325 samples) of samples were positive to antibodies against ORT and the remaining samples 27.8 percent (125 samples) were suspicious and non of samples were negative.In conclusion, the results of this study indicated that the prevalence of ORT antibodies is high in the broiler flocks of Ardabil province. Due to the economical importance of ORT infection it might be necessary to prepare a general prevention and control plan to tackle this emerging disease