1 research outputs found
Simultaneous Nanothermometry and DeepâTissue Imaging
Bright, stable, and biocompatible fluorescent contrast agents operating in the second biological window (1000â1350 nm) are attractive for imaging of deepâlying structures (e.g., tumors) within tissues. Ideally, these contrast agents also provide functional insights, such as information on local temperature. Here, waterâdispersible barium phosphate nanoparticles doped with Mn5+ are made by scalable, continuous, and sterile flame aerosol technology and explored as fluorescent contrast agents with temperatureâsensitive peak emission in the NIRâII (1190 nm). Detailed assessment of their stability, toxicity with three representative cell lines (HeLa, THPâ1, NHDF), and deepâtissue imaging down to about 3 cm are presented. In addition, their high quantum yield (up to 34%) combined with excellent temperature sensitivity paves the way for concurrent deepâtissue imaging and nanothermometry, with biologically wellâtolerated nanoparticles.ISSN:2198-384