At the origins of Pompeii: the plant landscape of the Sarno River floodplain from the first millennium bc to the ad 79 eruption

The ad 79 eruption of the Vesuvius severely affected the floodplain surrounding the ancient city of Pompeii, i.e. the Sarno River floodplain. The landscape was covered with volcaniclastic materials that destroyed the ecosystem but, at the same time, preserved the traces of former environmental conditions. This study provides—for the first time—a pollen sequence reconstructing the environmental evolution and the plant landscape of the Sarno floodplain between 900 and 750 cal bc and ad 79, i.e. before and during the foundation of the city, and during its life phases. Previous geomorphological studies revealed that the portion of the Sarno floodplain under the “Pompeii hill” was a freshwater backswamp with patchy inundated and dry areas. Palynology depicts a thin forest cover since the Early Iron Age, suggesting an open environment with a mosaic of vegetation types. The local presence of Mediterranean coastal shrubland, hygrophilous riverine forest and mesophilous plain forest is combined with the regional contribution of mountain vegetation through the sequence. Oscillations between inundated and wet ground characterized the studied area until the ad 79 eruption. Such a natural environment shows anthropogenic traits since pre-Roman times: pasturelands, cultivated fields and olive groves, which probably occupied drier soils. The most important change in the land use system was the introduction of cabbage cultivation in the fourth century bc and its intensification from the second century bc, when Roman influence grew. The presence of tree crops and of ornamental trees reveals the opulence of the Imperial age until the catastrophic eruption

3d collagen hydrogel promotes in vitro langerhans islets vascularization through ad-mvfs angiogenic activity

Adipose derived microvascular fragments (ad-MVFs) consist of effective vascularization units able to reassemble into efficient microvascular networks. Because of their content in stem cells and related angiogenic activity, ad-MVFs represent an interesting tool for applications in regenerative medicine. Here we show that gentle dissociation of rat adipose tissue provides a mixture of ad-MVFs with a length distribution ranging from 33–955 µm that are able to maintain their original morphology. The isolated units of ad-MVFs that resulted were able to activate transcriptional switching toward angiogenesis, forming tubes, branches, and entire capillary networks when cultured in 3D collagen type-I hydrogel. The proper involvement of metalloproteases (MMP2/MMP9) and serine proteases in basal lamina and extracellular matrix ECM degradation during the angiogenesis were concurrently assessed by the evaluation of alpha-smooth muscle actin (αSMA) expression. These results suggest that collagen type-I hydrogel provides an adequate 3D environment supporting the activation of the vascularization process. As a proof of concept, we exploited 3D collagen hydrogel for the setting of ad-MVF–islet of Langerhans coculture to improve the islets vascularization. Our results suggest potential employment of the proposed in vitro system for regenerative medicine applications, such as the improving of the islet of Langerhans engraftment before transplantation

Flow discharge measurement by a linear width contraction device

In this paper, the outflow process of a linear width contraction device for a free-flow condition is modeled using the dimensional analysis and the incomplete self-similarity condition. The proposed theoretical stage-discharge relationship is tested using measurements available in the literature. The proposed power stage-discharge equation is characterized by a value of the exponent close to 2 and a coefficient depending on the angle of the device sides with the channel bank. The proposed flume is characterized by simple construction, easy installation, low cost, and a good accuracy of the measured discharge (errors in the estimate ranging from - 3.84 to 1.9%). The deduced stage-discharge equation is characterized by errors in the estimate of discharge lower than or equal to & PLUSMN; 3% for 93.7% of the investigated cases. The main result of this paper is a stage-discharge relationship giving an accurate estimate of discharge but, at the same time, having the advantage of working regardless of the discharge coefficient estimate

Assessing a transitional and turbulent overland flow resistance law for surfaces with different roughness

The control and management of soil erosion phenomena caused by rainfall and runoff is a significant issue in sloping landscapes, especially if they are scarcely or not vegetated. The study of the relationship between soil roughness and flow resistance is a fundamental step in improving the knowledge of erosion processes. In this study, the suitability of a theoretically deduced flow resistance law, based on a power-velocity profile, was assessed by transitional and turbulent overland flow data obtained in laboratory and available in the literature. These measurements were obtained in a sloping (slope in the range 1-40 %) rectangular flume, testing the effects of six different roughness conditions. At first, for each investigated roughness condition, the available measurements were used to calibrate and test the equation relating the Gamma function of the velocity distribution, the flow Froude number, and the channel slope. For all the investigated conditions, this analysis allowed for demonstrating that the flow resistance law gives a reliable estimate of the Darcy-Weisbach friction factor, with errors <=+/- 5 % for 89.8-100 % of the examined cases with reference to the considered roughness condition. Then, using coefficients b (1.05) and c (0.562) of the Gamma function available in the literature, the roughness effect was exclusively attributed to the a coefficient. In this case, the friction factor values calculated by the flow resistance law, with b = 1.05 and c = 0.562 and the a values corresponding to the different roughness conditions, are characterized by errors <=+/- 5% for 70.4-100 % of the cases. Finally, the power relationships between the calibrated a, b, and c coefficients of the Gamma function, and Manning's n values, corresponding to the roughness of the investigated surfaces, pointed out that the a coefficient is the most affected by the roughness conditions, as the exponent of Manning's assumes the highest value. The significance of this research is due to the fact that a relevant issue in modeling overland flow is to define the resistance coefficient for variable roughness, especially the vegetated ones

Characterization of translationally controlled tumour protein from the sea anemone Anemonia viridis and transcriptome wide identification of cnidarian homologues

Gene family encoding translationally controlled tumour protein (TCTP) is defined as highly conserved among organisms; however, there is limited knowledge of non-bilateria. In this study, the first TCTP homologue from anthozoan was characterised in the Mediterranean Sea anemone, Anemonia viridis. The release of the genome sequence of Acropora digitifera, Exaiptasia pallida, Nematostella vectensis and Hydra vulgaris enabled a comprehensive study of the molecular evolution of TCTP family among cnidarians. A comparison among TCTP members from Cnidaria and Bilateria showed conserved intron exon organization, evolutionary conserved TCTP signatures and 3D protein structure. The pattern of mRNA expression profile was also defined in A. viridis. These analyses revealed a constitutive mRNA expression especially in tissues with active proliferation. Additionally, the transcriptional profile of A. viridis TCTP (AvTCTP) after challenges with different abiotic/biotic stresses showed induction by extreme temperatures, heavy metals exposure and immune stimulation. These results suggest the involvement of AvTCTP in the sea anemone defensome taking part in environmental stress and immune responses

Long term results of single high dose Stereotactic Body Radiotherapy in the treatment of primary lung tumors

Stereotactic body radiotherapy (SBRT) is a standard treatment for inoperable early-stage NSCLC, with local control rates comparable to surgical series. Promising results have been achieved utilizing a high single-dose schedule. The aim of our study was to evaluate long-term local control and toxicity in a series of patients treated with SBRT delivered in a single dose of 30 Gy. 44 patients affected by early stage NSCLC were treated with SBRT delivered in a single dose of 30 Gy. Survival and prognostic factors were retrospectively evaluated. Median follow-up was 34 months (range 3–81). Three- and 5-year local progression-free survival (LPFS) were 87.8% and 87.8% respectively (median 30 months; range 6–81 months), 3- and 5-year OS and CSS were 64.9% and 36.9%, 80.9% and 65.5%, respectively. Two (4.6%) cases of grade 3 pneumonitis occurred. At the univariate analysis lesion diameter ≤ 25 mm was predictive of better 5-year LPFS (95.8% versus 56.3%; p = 0.003) and 5-year PFS (69.8% versus 27.8%; p = 0.002). The results of our study indicated a high local control, survival and tolerability after a long-term follow-up with the use of SBRT 30 Gy single dose. Further prospective studies could better define the role of this regimen

Neural Crest-Derived Chondrocytes Isolation for Tissue Engineering in Regenerative Medicine

Chondrocyte transplantation has been successfully tested and proposed as a clinical procedure aiming to repair articular cartilage defects. However, the isolation of chondrocytes and the optimization of the enzymatic digestion process, as well as their successful in vitro expansion, remain the main challenges in cartilage tissue engineering. In order to address these issues, we investigated the performance of recombinant collagenases in tissue dissociation assays with the aim of isolating chondrocytes from bovine nasal cartilage in order to establish the optimal enzyme blend to ensure the best outcomes of the overall procedure. We show, for the first time, that collagenase H activity alone is required for effective cartilage digestion, resulting in an improvement in the yield of viable cells. The extracted chondrocytes proved able to grow and activate differentiation/dedifferentiation programs, as assessed by morphological and gene expression analyses

Hyperventilation and seizures in an adolescent female

A 16 year-old girl was admitted after suffering from recurrent episodes of dyspnea and stridor, cyanosis, loss of contact, stiffening of all four limbs, clenching of the jaw and eye retroversion that lasted for a few seconds to a minute, followed by slow recovery of consciousness without any loss of sphincter control. These symptoms began at the age of 11 and worsened over time. Prolonged rate corrected QT intervals was observed with an ECG. Two cardio-respiratory monitorings were performed (one during daytime hours while the patient was awake, and one at night time while the patient slept). Daytime recordings showed 17 central apnoeas and 97 central hypopneas, with an apnea-hypopnea index (AHI) of 13.2 events/hour, that were associated with severe oxyhemoglobin desaturation. In contrast, night time recordings were normal (AHI=1.1 events/hour). The patient underwent diurnal monitoring of transcutaneous pCO2(PtcCO2), transcutaneous O2(PtcO2), SpO2 and end tidal CO2 (PETCO2), with simultaneous monitoring of regional cerebral oxymetry (rSO2) which showed values of PtcCO2 between 8 and 15 mmHg, suggesting several episodes of marked hyperventilation. Twenty-nine episodes of severe arterial desaturation (SpO2<50%) were registered, all after the same number of apnea events, with ascent of PtcO2 up to 28 mmHg at the end of apnea. During the final phase of apnea, the patient showed cyanosis, contact disturbance, grimaces, oral movements of rhyme, and, on three occasions, partial seizures. A mask was packaged and applied daytime to the face of the patient during episodes of hyperventilation to prevent, together with a psychiatric follow up, rapid falls of PaCO2 levels determining central apnoeas