Flipgrid – ein Hausaufgabenprojekt. Neurodidaktische Erkenntnisse in praktischer Umsetzung durch Blended Learning

Im vorliegenden Forschungsprojekt wird ein Unterrichtsexperiment vorgestellt, in dem zwei aktuelle Forschungsbereiche in der Didaktik verknüpft werden: neurodidaktische Erkenntnisse und digitale Medien. Mithilfe der virtuellen und interaktiven Internet-Plattform "Flipgrid" sollten Studierendengruppen der Westböhmischen Universität in Pilsen Hausaufgaben in Form von verschiedenen Aufgabentypen bearbeiten und aufsprechen. Aufgrund einer Fragebogenauswertung wird dann festgestellt, ob das angewandte Blended Learning bei den Studierenden Anklang gefunden und die Motivation, Hausaufgaben zu erledigen, erhöht hat.This research project focuses on ateaching experiment in which two current research areas in didactics are linked: findings of neurodidactic and digital media. With the help of the virtual and interactive internet platform "Flipgrid", various student groups of the University of West Bohemia in Pilsen were supposed to work on homework assignments with different types of tasks which were then recorded by the students. Based on aquestionnaire evaluation, it is then determined whether the students found the applied blended learning beneficial and whether it increased their motivation to do their homework

Second generation silver(I)-mediated imidazole base pairs

The imidazole–Ag(I)–imidazole base pair is one of the best-investigated artificial metal-mediated base pairs. We show here that its stability can be further improved by formally replacing the imidazole moiety by a 2-methylimidazole or 4-methylimidazole moiety. A comparison of the thermal stability of several double helices shows that the addition of one equivalent of Ag(I) leads to a 50% larger increase in the melting temperature when a DNA duplex with methylated imidazole nucleosides is applied. This significant effect can likely be attributed to a better steric shielding of the metal ion within the metal-mediated base pair

Solution structure of a DNA double helix with consecutive metal-mediated base pairs

Metal-mediated base pairs represent a powerful tool for the site-specific functionlization of nucleic acids with metal ions. The development of applications of the metal-modified nucleic acids will depend on the availability of structural information on these double helices. We present here the NMR solution structure of a self-complementary DNA oligonucleotide with three consecutive imidazole nucleotides in its centre. In the absence of transition-metal ions, a hairpin structure is adopted with the artifical nucleotides forming the loop. In the presence of Ag(I) ions, a duplex comprising three imidazole-Ag+-imidazole base pairs is formed. Direct proof for the formation of metal-mediated base pairs was obtained from (1)J(N-15,Ag-107/109) couplings upon incorporation of N-15-labelled imidazole. The duplex adopts a B-type conformation with only minor deviations in the region of the artifical bases. This work represents the first structural characterization of a metal-modified nucleic acid with a continuous stretch of metal-mediated base pairs