Assessment of How Natural Stand Structure for Narrow Endemic Cedrus brevifolia Henry Supports Silvicultural Treatments for Its Sustainable Management

Cedrus brevifolia Henry is a narrow endemic tree species of Cyprus flora. The objectives of this study are to develop silvicultural treatments for the conservation of the species formations based on the stand structure analysis of C. brevifolia natural forest and to present the characteristics of the first application of the treatments through silvicultural interventions. Six structural types were distinguished in C. brevifolia formations in the study area located in the state forest of Paphos. For each structural type, six circular plots of approximately 500 m2 were established. In each plot, various measurements and estimations were recorded. Then, silvicultural interventions were applied in the plots of the mixed C. brevifolia formations. In the formations of C. brevifolia a great number of trees grow in the understory. In the very productive and in the poorly productive sites C. brevifolia occurs only in pure formations. The basal area of C. brevifolia in pure formations ranges from 19.04 m2·ha-1 in poorly productive sites to 38.49 m2·ha-1 in fairly productive sites. Cedrus brevifolia is the most competitive species of the study area as a result of both shade tolerance and the wide range of its site sensitivity behavior. The climax of the study area are the pure stands of C. brevifolia having an understory of Quercus alnifolia Poech and a sparse occurrence of Pinus brutia Ten., mainly in moderately productive sites. Forest practice has to, as much as possible, unite species formations in order to create extensive areas of C. brevifolia formations

Mediterranean Islands Hosting Marginal and Peripheral Forest Tree Populations: The Case of Pinus brutia Ten. in Cyprus

Mediterranean islands have served as important Tertiary and glacial refuges, hosting important peripheral and ecologically marginal forest tree populations. These populations, presumably harboring unique gene complexes, are particularly interesting in the context of climate change. Pinus brutia Ten. is widespread in the eastern Mediterranean Basin and in Cyprus in particular it is the most common tree species. This study evaluated genetic patterns and morphoanatomical local adaptation along the species geographical distribution and altitudinal range in Cyprus. Analysis showed that the Cyprus population of P. brutia is a peripheral population with high genetic diversity, comprised of different subpopulations. Evidence suggests the presence of ongoing dynamic evolutionary processes among the different subpopulations, while the most relic and isolated subpopulations exhibited a decreased genetic diversity compared to the most compact subpopulations in the central area of the island. These results could be the consequence of the small size and prolonged isolation of the former. Comparing populations along an altitude gradient, higher genetic diversity was detected at the middle level. The phenotypic plasticity observed is particularly important for the adaptive potential of P. brutia in an island environment, since it allows rapid change in local environmental conditions

Complex fine-scale phylogeographical patterns in a putative refugial region for Fagus sylvatica (Fagaceae)

Broad-scale plastid (chloroplast) DNA studies of beech (Fagus sylvatica) populations suggest the existence of glacial refugia and introgression zones in south-eastern Europe. We choose a possible refugium of beech in northern Greece, Mt. Paggeo, which hosts a private plastid haplotype for beech, to conduct a fine-scale genetic study. We attempt to confirm or reject the hypothesis of the existence of a small-scale refugium and to gain an understanding of the ecological and topographical factors affecting the spatial distribution of plastid haplotypes in the area. Our results reveal a high haplotype diversity on Mt. Paggeo, but the overall distribution of haplotypes shows no significant correlation with the ecological characteristics of the beech forests. However, the private haplotype is found at high frequencies in beech forests located in or near ravines, having a high spatial overlap with a relict vegetation type occurring in ecological conditions found mainly in ravines. This result emphasizes the importance of topography in the existence of glacial refugia in the wider area. Furthermore, haplotypes originating from two more widespread beech lineages in Greece are found on Mt. Paggeo, indicating a possible mixing of populations originating from a local refugium with populations from remote refugia that possibly migrated into the area after the last glaciation. © 2014 The Linnean Society of London

Significant patterns of fine-scale spatial genetic structure in a narrow endemic wind-dispersed tree species, Cedrus brevifolia Henry

Cedrus brevifolia is a narrowly distributed conifer species, currently limited to a single mountain in Cyprus, growing in restricted habitats on sites of different densities and sizes. This study assessed the influence of seed and pollen dispersal, as well as the effect of demographic and genetic features on the magnitude of fine-scale spatial genetic structure (SGS). Sampling was performed in 11 plots where 50 neighboring adult trees were sampled from each plot, while biparentally and paternally inherited genomes were used for analysis with microsatellites. Fine-scale SGS was significant but showed contrasting patterns among plots. Although the magnitude of SGS in C. brevifolia mainly results from restricted seed dispersal, short-distance pollen dispersal could also explain fine-scale SGS in some plots, which is rather uncommon in wind-pollinated conifer species. The lack of a general and consistent trend of SGS among plots and between genomes indicates that pollen and seed dispersal varies at plot level. The complex SGS patterns in C. brevifolia may result from the unequal ratio of male and female strobilies of trees within the same plots, at different reproductive periods. Demographic features such as habitat fragmentation did not influence the magnitude of SGS in C. brevifolia, whereas low tree aggregation reduced it. Further, the significant correlation observed between linkage disequilibrium (LD) and plots with significant SGS supports the assumption that under specific conditions, LD is likely to be caused by the magnitude of SGS