Effects of yeast extract and methyl jasmonate on the enhancement of solasodine biosynthesis in cell cultures of Solanum hainanense Hance

In this work, the effects of the elicitors methyl jasmonate (MeJA) and yeast extract (YE) on the growth and solasodine production of Solanum hainanense cells were investigated. The results showed that various concentrations of MeJA (50-250 µM) and YE (1-4 g/L) have different eliciting influences. The increase of solasodine content induced by the elicitation of 3 g/L of YE and 50 µM of MeJA at the beginning of cell culture was about 1.9- and 1.3-fold, respectively, as compared with that of the non-elicitated cells. In general, YE (biotic elicitor) was more effective in enhancing solasodine production than MeJA (abiotic elicitor)

Functional Complementation Studies and Analysis of Downstream Regulatory Gene Expression Networks of Trichome Trimeric Complex

In Arabidopsis, previous genetic analysis has revealed that trichome initiation is positively mediated by a trimeric activation complex comprised of an R2R3-MYB protein GLABRA1 (GL1), a basic helix-loop-helix (bHLH) GLABRA3 (GL3) which acts redundantly with its close homolog ENHANCER OF GLABRA3 (EGL3), and a WD40 protein TRANSPARENT TESTA GLABRA1 (TTG1). We studied the functionality of four individual cotton genes MYB2, TTG3, DEL65 and DEL61 from diploid cotton A and D genomes, which show high similarity in sequence with GL1, TTG1, GL3 and EGL3, respectively, in their respective Arabidopsis glabrous mutants. Our complementation assays proved that transgenic lines with MYB2, TTG3 from diploid genomes A and D could rescue the trichomeless phenotype of gl1-1 and ttg1-1, respectively. However, DEL61 from both the species could not rescue this phenotype of gl3-1 egl3-77439 double mutant. Interestingly, the DEL65 from A species rescued gl3-1 egl3-77439 double mutant but not from D diploid species. Comparative quantitative PCR analysis of the downstream regulatory network genes showed a similar pattern for MYB, TTG3 complemented lines from A- and D- diploid species. Comparative analysis of the DEL65 from A- (rescued the trichomeless phenotype) and D- (did not rescued the trichomeless phenotype) showed differential expression of regulatory network genes between these two lines. These results suggested that MYB2, TTG3 and DEL65, when expressed in Arabidopsis, regulated the regulatory network genes during the trichome initiation process

On Phase Transitions of Nuclear Matter in the Nambu-Jona-Lasinio Model

Within the Cornwall - Jackiw - Tomboulis (CJT) approach a general formalism is established for the study of asymmetric nuclear matter (ANM) described by the Nambu-Jona-Lasinio (NJL) model. Restricting to the double-bubble approximation (DBA)we determine the bulk properties of ANM, in particular, the density dependence of the nuclear symmetry energy, which is in good agreement with data of recent analyses

Mini review: Micro RNA in nasopharyngeal carcinoma

Nasopharyngeal carcinoma (NPC) is a highly invasive and malignant tumor of the nasopharynx, the uppermost region of the pharynx, gravitating toward Southeast Asia, especially Vietnam. The etiology of NPC is suggested to be involved to several genetic susceptibility and environmental factors. miRNAs (microRNA), the short non-coding RNA molecular, have been reported that played important roles, as oncogene and/or tumor suppressor gene, in progression and development of NPC. In this review, we summarized the role of miRNAs in NPC and their potential use as biomarkers for early prognosis and diagnosis of NPC as well as therapeutic agent of NPC in future

In vitro bioactivities of Codonopsis javanica root extract from Kon Tum province, Vietnam

Dangshen Codonopsis javanica exhibits invaluable medicinal properties in herbal remedies; however, there has currently not been much specific analysis of the phytochemicals and bioactivities of this plant. The root ethanol extract of C. javanica contains substances such as saponins, phenolic acids, terpenoids, and alkaloids. It displays an antibacterial effect against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus with the IC50 values of 150, 100, 150, and 90 μg/mL, respectively. The antioxidant capacity of the root extract was also observed with an IC50 value of 46.8 ± 6.8 μg/mL. Furthermore, the extract exhibits activity on human cancer cell lines HepG2 (IC50 = 83.6 ± 2.7 μg/mL) and MCF-7 (IC50 = 95.3 ± 2.3 μg/mL). Hence, this study provides the basic data for further research on the bioactivities of natural compounds of Dangshen C. javanica for the first time

Evaluation of EBNA-1 (epstein-barr virus nuclear antigen-1) gene prevalence in nasopharyngeal carcinoma in Vietnamese patients

This study examined the presence of Epstein-Barr virus (EBV) in nasopharyngeal carcinoma (NPC) based on the detection of EBNA-1 (Epstein-Barr virus nuclear antigen-1) by Polymerase Chain Reaction (PCR), in Vietnamese population. Firstly, we systematically analyzed the mean of percentage weighted of the presence of EBNA-1 in previous relevant studies. Experimentally, 31 nasopharyngeal cancer biopsies and 20 healthy samples were enrolled in current to evaluate the frequency of candidate genes. As the results, the frequency of EBNA-1 was 77.42%, whereas, none of any cases of healthy samples were found to positive to target gene. The p value < 0.05 (p = 0.0001) showed that it was significant correlation between the presence of this candidate gene and nasopharyngeal cancer. Moreover, a high odds ratio (OR) and relative risk (RR) of candidate gene, (OR = 68.16, RR = 2.41) were calculated. Therefore, the detection of EBNA-1, which performed by PCR, could serve as a good supplement to early diagnosis and prognosis of NPC in Vietnamese population

GLUCOSIDES AND UREA DERIVATIVES FROM THE SEEDS OF SCAPHIUM MACROPODUM (MIQ.) BEUMÉE

Five known compounds {carbonylbis[imino(6-methyl-3,1-phenylenel)]}bis[carbamic acid] dimethyl ester (1), (1'R,3'S,5'R,8'S,2E,4E-dihydrophaseic acid) 3'-O-beta-D-glucopyranoside (2), 3-methylbutan-1-ol beta-D-glucopyranoside (3), astragalin (4) and daucosterol (5) were isolated from the methanol extract of the seeds of Scaphium macropodum (Miq.) Beumée. The structures of the isolated compounds were elucidated by the spectroscopic methods including NMR and MS, and also by comparison with the literature data. Compounds 1-3 were isolated from this plant for the first time

TextANIMAR: Text-based 3D Animal Fine-Grained Retrieval

3D object retrieval is an important yet challenging task, which has drawn more and more attention in recent years. While existing approaches have made strides in addressing this issue, they are often limited to restricted settings such as image and sketch queries, which are often unfriendly interactions for common users. In order to overcome these limitations, this paper presents a novel SHREC challenge track focusing on text-based fine-grained retrieval of 3D animal models. Unlike previous SHREC challenge tracks, the proposed task is considerably more challenging, requiring participants to develop innovative approaches to tackle the problem of text-based retrieval. Despite the increased difficulty, we believe that this task has the potential to drive useful applications in practice and facilitate more intuitive interactions with 3D objects. Five groups participated in our competition, submitting a total of 114 runs. While the results obtained in our competition are satisfactory, we note that the challenges presented by this task are far from being fully solved. As such, we provide insights into potential areas for future research and improvements. We believe that we can help push the boundaries of 3D object retrieval and facilitate more user-friendly interactions via vision-language technologies.Comment: arXiv admin note: text overlap with arXiv:2304.0573