80 research outputs found

    Isolation and selection of a strain of bacillus subtilis group for high antagonistic activity against Colletotrichum scovilleicausing chilli anthracnose disease in Ho Chi Minh City

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    Chilli anthracnose disease caused by Colletotrichum spp. has heavily damaged the quality and yield production of chili around the world. In Viet Nam, many chili growing regions namely Ho Chi Minh City have been enormously affected by the disease for many years. Nowadays, a biological control using antagonistic microorganisms to prevent plant pathogens is becoming increasingly popular due to its safety and effectiveness. In particular, bacteria belonging to Bacillus subtilis group has been proven to have antagonistic ability against pathogenic fungi. Therefore, this study was conducted to isolate and select bacteria of Bacillus subtilis group which show high antagonistic activity against the fungus Colletotrichum scovillei causing Chilli anthracnose disease in Ho Chi Minh City. From five soil samples, the study isolated 22 candidate strains that initially categorized as Bacillus subtilis group. Out of 22 isolates, the BHCM8.3 strain showed the best inhibitory effect on the growth of Colletotrichum scovillei in the dual-culture agar overlay method (antagonistic effectiveness is 81.58% after 15 days). 16S ribosomal DNA (rDNA)-based molecular identification reveals that the BHCM8.3 strain is completely identical to the bacterium Bacillus subtilis (100%)

    Evaluation of Trichoderma spp. and Bacillus subtilis against Pythium vexans causes root rot on black pepper

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    Pythium spp. are well known as one of the main pathogens causing quick wilt disease of black pepper and severely reducing pepper yield (Shashidhara, 2007). Many treatment methods have been used to prevent root rot of black pepper such as chemical and biological methods. However, chemical treatment often has low effectiveness, harmfulness and environmental-unfriendliness. Meanwhile thanks to the long efficiency and eco-friendliness, biological agents have been increasingly using. In fact, Trichoderma spp. and Bacillus spp. were demonstrated that they possibly had the good antagonistic property against Pythium spp. because of their extracellular enzymes including glucanase, chitinase, cellulose… (Amrita et al., 2016; Anita et al., 2012; Najwa et al., 2016). The results of this study showed that in vitro the suspension containing 106 zoospores/ml of strain Pythium vexans P6 caused root rot of black pepper with the highest disease index and disease rate among 11 strains Pythium vexans. In addition, through this research antagonist effects of twelve Trichoderma strains and five Bacillus subtilis strains against Pythium vexans in vitro were also revealed. After 6 days of dual culture of Trichoderma spp. and Pythium vexans P6, the percentage of inhibition was from 40% to 90%. Almost all strains of Trichoderma spp. could completely inhibit the growth of the pathogen after 8 days of dual culture. Besides, all five strains Bacillus subtilis revealed the growth inhibition against Pythium vexans P6 on agar dish; however, the proportion of inhibition on the development of pathogen was only 22,69% till 27,67% after 6 days of dual culture, lower than that of Trichoderma spp.

    PHÂN LOẠI CHỦNG VI KHUẨN BTLP1 CÓ KHẢ NĂNG PHÂN HỦY PHENOL BẰNG PHƯƠNG PHÁP PHÂN TÍCH TRÌNH TỰ NUCLEOTIT CỦA ĐOẠN GEN 16S rARN

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    Chủng BTLP1 được chúng  tôi phân  lập  từ nguồn nước  thải có chứa phenol của khu công nghiệp vừa và nhỏ Từ Liêm Hà Nội có màu hồng, tròn, đường kính từ 2 - 3 mm. Dưới kính hiển vi điện  tử quét,  tế bào có dạng hình que, kích  thước từ 0,6 – 0,8 µm × 3,6 – 4,4 µm. Dựa vào việc so sánh trình  tự đoạn gen 16S rRNA, chủng BTLP1 có độ  tương đồng cao (97%) với các chủng  thuộc  chi  Rhodococcus,  đặc  biệt  chúng  có  độ  tương  đồng  cao  với  loài  Rhodococcus pyridinovorans mã số AF173005. Chủng vi khuẩn này được đặt tên là Rhodococcus sp. BTLP1. và đã được đăng ký  trên ngân hàng Genbank  (NCBI) với mã số  là JF750921. Chủng vi khuẩn Rhodococcus sp. BTLP1 có khả năng phân hủy 92,5 % phenol với nồng độ ban đầu là 150 ppm phenol tại 30 oC sau 7 ngày nuôi cấ

    ẢNH HƯỞNG CỦA HEMA ĐẾN QUÁ TRÌNH TỔNG HỢP VÀ TÍNH NHẠY NHIỆT CỦA POLY(NIPAM-CO-HEMA)

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    Copolyme của poly(N-isopropylacrylamit-co-hydroxyethyl methacrylat) [P(NIPAM-co-HEMA)] được tổng hợp từ quá trình đồng trùng hợp các monome N-isopropylacrylamit (NIPAM) và 2-hydroxyethyl methacrylat (HEMA) với tỷ lệ mol xác định. Hằng số đồng trùng hợp của HEMA cao hơn so với NIPAM khi xác định theo phương pháp Kelen-Tudos (rNIPAM= 0.05; rHEMA=0.5). Cấu trúc đặc trưng của copolyme được xác định bằng phổ hồng ngoại FTIR, nhiệt độ dung dịch tới hạn dưới (LCST) của copolyme tăng khi tăng hàm lượng HEMA

    BƯỚC ĐẦU XÁC ĐỊNH HÀM LƯỢNG MỘT SỐ KIM LOẠI NẶNG TRONG MÔI TRƯỜNG NƯỚC SÔNG HỒNG

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    Environment quality, especially aquatic environment is increasingly being interested. TThis paper presents the initial results of the monthly observations for the period from January 2012 to December 2012 on some heavy metal contents in the Red River system at the four hydrological stations in Ha Noi, Hoa Binh, Vu Quang and Yen Bai. The monitoring results showed that heavy metal concentrations in the Red River water varied in a high range: Cu: 10 – 80 mg/l; Zn: 2 – 88 mg/l; Cr: 0,2 – 5,1 mg/l; Pb: 2  - 107 mg/l; Cd: : 2 – 12 mg/l; Mn: 2 - 35 mg/l; Fe: 160 – 950 mg/l. Most of the mean values of heavy metal contents at the four monitoring sites were lower than the ones of  the Vietnamese standard limits for surface water quality, QCVN 08:2008/BTNMT. However, at several time during the observation period, the contents of some heavy metals such as Fe, Cd and Pb exceeded the Vietnamese standard limits. The results shows that the water quality of the Red River needs more be frequently and systhematically observe
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